Modeling Honey Adulteration by Processing Microscopic Images Using Artificial Intelligence Methods

Document Type : Original Research

Authors
Department of Mechanics of Biosystems Engineering, Razi University, Kermanshah, Islamic Republic of Iran.
Abstract
The aim of this study was to determine the authenticity of honey by processing microscopic images and obtaining an algorithm for classifying various honey frauds. In this study, sucrose, fructose, and fructose-glucose solution at a ratio of 0.9 were used to make honey adulteration. The level of adulterated honey was based on the weight percentages of 2.5, 5, 7.5, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 by stirring. Different samples were imaged under a microscope. Each image was processed in 33 monochrome color spaces and 15 parameters were extracted from it. The three main and effective parameters of various color spaces were selected using sensitivity analysis for modeling honey fraud by adaptive Fuzzy Neural Inference System (ANFIS), Artificial Neural Network (ANN), and response surface methodology. Various criteria were used to evaluate the performance of the models such as coefficient of determination, mean square error, sum of squared estimate of errors, and mean absolute errors. The results showed that the determination coefficient and the mean square error of the artificial neural network model was 0.974 and 0.0024, respectively. Finally, using the desirability function, the artificial neural network model was selected as the best model due to less prediction error values and desirability of 0.948.

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1. Standard CA, Revised Codex Standard for Honey, Rev. 2. 24th Session of the Codex Alimentarius (2001).
2. Li S, Shan Y, Zhu X, Zhang X and Ling G, Detection of honey adulteration by high fructose corn syrup and maltose syrup using Raman spectroscopy. J Food Compost Anal 28:69-74 (2012).
3. Tosun M, Detection of adulteration in honey samples added various sugar syrups with 13 C/12 C isotope ratio analysis method. Food Chem 138:1629-1632 (2013).
4. Agila A and Barringer S, Effect of adulteration versus storage on volatiles in unifloral honeys from different floral sources and locations. J Food Sci 78:184-191 (2013).
5. Anthony C and Balasuriya D, Electronic Honey Quality Analyser. Engineer: Journal of the Institution of Engineers, Sri Lanka 49:41-47 (2016).
6. Cordella C, Faucon J-P, Cabrol-Bass D and Sbirrazzuoli N, Application of DSC as a tool for honey floral species characterization and adulteration detection. JTAC 71:279-290 (2003).
7. Du B, Wu L, Xue X, Chen L, Li Y, Zhao J and Cao W, Rapid screening of multiclass syrup adulterants in honey by ultrahigh-performance liquid chromatography/quadrupole time of flight mass spectrometry. J Agric Food Chem 63:6614-6623 (2015).
8. Zakaria A, Shakaff AYM, Masnan MJ, Ahmad MN, Adom AH, Jaafar MN, Ghani SA, Abdullah AH, Aziz AHA and Kamarudin LM, A biomimetic sensor for the classification of honeys of different floral origin and the detection of adulteration. Sensors 11:7799-7822 (2011).
9. Salvador L, Guijarro M, Rubio D, Aucatoma B, Guillén T, Vargas Jentzsch P, Ciobotă V, Stolker L, Ulic S and Vásquez L, Exploratory monitoring of the quality and authenticity of commercial honey in Ecuador. Foods 8:105 (2019).
10. Du C-J and Sun D-W, Recent developments in the applications of image processing techniques for food quality evaluation. Trends Food Sci Technol 15:230-249 (2004).
11. Esteki M, Farajmand B, Kolahderazi Y and Simal-Gandara J, Chromatographic fingerprinting with multivariate data analysis for detection and quantification of apricot kernel in almond powder. Food Analytical Methods 10:3312-3320 (2017).
12. Esteki M, Simal-Gandara J, Shahsavari Z, Zandbaaf S, Dashtaki E and Vander Heyden Y, A review on the application of chromatographic methods, coupled to chemometrics, for food authentication. Food Control 93:165-182 (2018).
13. Esteki M, Shahsavari Z and Simal-Gandara J, Use of spectroscopic methods in combination with linear discriminant analysis for authentication of food products. Food Control 91:100-112 (2018).
14. Yang M, Gao Y, Liu Y, Fan X, Zhao K and Zhao S, Broadband dielectric properties of honey: effect of water content. Journal of Agricultural Science and Technology 20:685-693 (2018).
15. Fahim Danesh M and Bahrami ME, Evaluation of adulteration in sesame oil using Differential Scanning Calorimetry. Food Science and Technology 13:81-89 (2015).
16. Khorsandmanesh S, Gharachorloo M, Bahmaie M, Moghaddam AZ and Azizinezhad R, Sterol and Squalene as Indicators of Adulteration of Milk Fat with Palm Oil and Its Fractions. Journal of Agricultural Science & Technology 22 (2020).
17. Shafiee S, Polder G, Minaei S, Moghadam-Charkari N, Van Ruth S and Kuś PM, Detection of Honey Adulteration using Hyperspectral Imaging. IFAC-PapersOnLine 49:311-314 (2016).
18. Cseke I, Fazekas Z and Holka T, Honey qualification—an application of the ARGUS image processing system. Microprocessors and Microsystems 17:219-222 (1993).
19. Kerkvliet J, Shrestha M, Tuladhar K and Manandhar H, Microscopic detection of adulteration of honey with cane sugar and cane sugar products. Apidologie 26:131-139 (1995).
20. Anjos O, Iglesias C, Peres F, Martínez J, García Á and Taboada J, Neural networks applied to discriminate botanical origin of honeys. Food Chem 175:128-136 (2015).
21. D’Ávila V, Aguiar-Menezes E, Gonçalves-Esteves V, Mendonça C, Pereira R and Santos T, Morphological characterization of pollens from three Apiaceae species and their ingestion by twelve-spotted lady beetle (Coleoptera: Coccinellidae). Braz J Biol 76:796-803 (2016).
22. Asadi M, Beet-sugar handbook. John Wiley & Sons (2006).
23. Bidin N, Zainuddin NH, Islam S, Abdullah M, Marsin FM and Yasin M, Sugar Detection in Adulterated Honey via Fiber Optic Displacement Sensor for Food Industrial Applications. IEEE Sens J 16:299-305 (2016).
24. Gonzalez RC and Woods RE, Digital image processing prentice hall. Upper Saddle River, NJ (2002).
25. Mostafaei M, ANFIS models for prediction of biodiesel fuels cetane number using desirability function. Fuel 216:665-672 (2018).
26. Myers RH, Montgomery DC and Anderson-Cook CM, Response surface methodology: process and product optimization using designed experiments. John Wiley & Sons (2016).
27. Ou W-J, Meng Y-Y, ZHANG X-Y and KONG M, Application of UV-visible absorption spectroscopy and principal components-back propagation artificial neural network to identification of authentic and adulterated honeys. Chinese Journal of Analytical Chemistry 39:1104-1108 (2011).
28. Irudayaraj J, Xu R and Tewari J, Rapid determination of invert cane sugar adulteration in honey using FTIR spectroscopy and multivariate analysis. J Food Sci 68:2040-2045 (2003).
29. Chen Q, Qi S, Li H, Han X, Ouyang Q and Zhao J, Determination of rice syrup adulterant concentration in honey using three-dimensional fluorescence spectra and multivariate calibrations. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 131:177-182 (2014).
30. Ouyang Q, Identification of Adulterated Honey Based on Three-Dimensional Fluorescence Spectra Technology. Spectroscopy and Spectral Analysis 33:1626-1630 (2013).