Aquatic Extract of Camellia sinensis L. as the Inducer of Cucumber Systemic Resistance to Bemisia tabaci Gennadius (Hem.: Aleyrodidae)

Document Type : Original Research

Authors
A. Rajabpour 1
M. R. Zare Bavni 2
1 Department of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Islamic Republic of Iran.
2 Department of Horticultural Sciences, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Islamic Republic of Iran.
Abstract
Whitefly, Bemisia tabaci Gennadius (Hem.: Aleyrodidae), is a globally important pest of many vegetables including cucumber. In this study, for the first time, the effect of tealeaf extract, Camellia sinensis (L.) Kuntze (Theacea), on the induction of a plant (cucumber) resistance to a phloem feeder insect, namely, B. tabaci, was investigated under laboratory conditions. The cucumber plants were irrigated using different concentrations of C. sinensis leaf extracts (0, 0.001, 0.003, 0.006, and 0.009 g mL-1). Life table parameters of B. tabaci were determined on the treated and control plants using two-sex life table method. Our data indicated that the whitefly longevity at the concentrations 0.006 and 0.009 g mL-1 were significantly more than control. Moreover, net Reproductive rate (R0) and intrinsic rate of increase (r) were 68.8 and 47.7% or 82.6 and 79.1% lower than control at the concentrations 0.006 or 0.009 g mL-1, respectively. Therefore, these concentrations (0.006 and 0.009 g mL-1) caused significant adverse effects on the biological traits of the pest implying the induction of cucumber resistance to the whitefly. Chemical analyses of the treated and control plants indicated that the treatment with tea extract led to significant increase in total tannin, phenol and flavonoid contents of treated cucumber, while considerably reducing alkaloid and saponin contents. Totally, the concentrations 0.006 and 0.009 g mL-1 of aquatic extract of tea can be used as resistance inducer of cucumber to the whitefly.

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Abdolmaleki, F. 2016. Chemical Analysis and Characteristics of Black Tea Produced in North of Iran. J. Food Biosci. Technol., 6(1): 23-32.
Abe, M. and Matsuda, K. 2000. Feeding responses of four phytophagous lady beetle species (Coleoptera: Coccinellidae) to cucurbitacins and alkaloids. Appl. Entomol. Zool., 35(2): 257-264.
Azadi, F., Rajabpour, A., Lotfi Jalal Abadi, A. and Mahjoub, M. 2018. Resistance of tomato cultivars to Tuta absoluta (Lepidoptera: Gelechiidae) under field condition. J. Crop Protect., 7(1): 87-92.
Banihashemi, A.S., Seraj, A.A., Yarahmadi, F. and Rajabpour, A. 2017. Effect of host plants on predation, prey preference and switching behaviour of Orius albidipennis on Bemisia tabaci and Tetranychus turkestani. Inter. J. Trop Insect Sci., 37(3): 176-182.
Baysal, O., Laux, P. and Zeller, W. 2001. Further studies on the induced resistance (IR) effect of plant extract from Hedera helix against fire blight (Erwinia amylovora). IX International Workshop on Fire Blight, October, pp. 273-277.
Bennett, R.N. and Wallsgrove, R.M. 1994. Secondary metabolites in plant defense mechanisms. New Phytol. 127(4): 617-633.
Bernays, E.A. 1981. Plant tannins and insect herbivores: an appraisal. Ecol. Entomol., 6(4): 353-360.
Boor, J.Y., Chen, H.Y. and Yen, G.C. 2006. Evaluation of Antioxidant Activity and Inhibitory Effect on Nitric Oxide Production of Some Common Vegetables. J. Agri. Food Chem., 54: 1680-1686.
Burden, R.L. and Faires, J.D. 2005. Numerical analysis. 8th ed. Thomson, Belmont, Canada
Chi, H., and Liu, H. 1985. Two new methods for the study of insect population ecology. Bul. Instit. Zool. Acad. Sin., 24: 225–240.
Chi H. 1988. Life-table analysis incorporating both sexes and variable development rate among individuals. Environ. Entomol., 17: 26–34.
Choudhary, D.K., Prakash, A. and John, B.N. 2007. Induced systemic resistance (ISR) in plants: mechanism of action. Indian J. Microbiol., 47(4): 289-297.
Efron, B., Tibshirani, R.J. 1993. An introduction to the bootstrap. Chapman and Hall, New York, USA.
Epstein, E. 1972. Mineral Nutrition of Plants: Principles and Perspectives. John Wiley and Sons, New York, USA.
Ezeonu, C.M. and Ejikeme, C.M. 2016. Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods. New J. Sci.: 1-9.
Frederick, N. and Mani, J. 2016. Total Phenolic Content, Total Flavonoid Content, Total Tannin Content and Antioxidant Activity of Musa acuminate. J. Inter. Acad Res. Multidiscip., 4(1): 300-308.
Goławska, S. and Łukasik, I. 2009. Acceptance of low-saponin lines of alfalfa with varied phenolic concentrations by pea aphid (Homoptera: Aphididae). Biologia. 64(2): 377-382.
Golawska, S., Kapusta, I., Lukasik, I. and Wojcicka A. 2008a. Effect of phenolics on the pea aphid, Acyrthosiphon pisum (Harris) population on Pisum sativum L. (Fabaceae). Pesticides. 3-4: 71-77.
Goławska, S., Łukasik, I. and Leszczyński, B. 2008b. Effect of alfalfa saponins and flavonoids on pea aphid. Entomol. Exp. Appl., 128(1): 147-153.
Goodman, D. 1982. Optimal life histories, optimal notation, and the value of reproductive value. Am. Natural., 119: 803- 823.
Hedin, P.A., Maxwell, F.G. and Jenkins, J.N. 1974. Insect plant attractants, feeding stimulants, repellents, deterrents and other related factors affecting insect behavior. Proceedings, Summer Institute on Biological Control of Plants and Diseases (Ed. by FG Maxwell and FA Harris), pp. 494e527. Jackson, Mississippi: University Press.
Heldt, H.W. and Piechulla, B. 2010. Plant Biochemistry, 4th Edition. Elsevier Academic Press, London, UK.
Inagaki, H., Yamaguchi, A., Kato, K., Kageyama, C. and Iyozumi, H. 2011. Induction of systemic resistance to anthracnose in cucumber by natural components of Allium vegetables and shiitake mushrooms. Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, ed. A. Méndez-Vilas (Sain: Formatex Research Center), 728-735.
Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64: 555-559.
Kagale, S., Marimuthu, T., Kagale, J., Thayumanavan, B. and Samiyappan, R. 2011. Induction of systemic resistance in rice by leaf extracts of Zizyphus jujuba and Ipomoea carnea against Rhizoctonia solani. Plant Sig. Behav., 6(7): 919-923.
Kumar, A., Nair, A.G.C., Reddy, A.V.R. and Garg, A.N. 2005. Availability of essential elements in Indian and US tea brands. Food Chem., 89: 441–448.
Latha, P., Anand, T., Ragupathi, N., Prakasam, V. and Samiyappan, R. 2009. Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani. Biol. Contr., 50(2): 85-93.
Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X., Kong, M., Li, L., Zhang, Q., Liu, Y., Chen, H., Qin, W., Wu, H. and Chen, S. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules. 21(10): 1-19.
Mali, S. and Borges, R.M. 2003. Phenolics, fibre, alkaloids, saponins, and cyanogenic glycosides in a seasonal cloud forest in India. Biochem. Sys. Ecol., 31: 1221–1246.
Moctezuma, C., Hammerbacher, A., Heil, M., Gershenzon, J., Méndez-Alonzo, R. and Oyama, K. 2014. Specific polyphenols and tannins are associated with defense against insect herbivores in the tropical oak Quercus oleoides. J. Chem.l Ecol., 40(5): 458-467.
Mound, L.A. and Halsey, S.H. 1978. Whitefly of the World: a Systematic Catalogue of the Aleyrodidae (Homoptera) with Host Plant and Natural Enemy Data. Wiley, New York, USA.
Murray, K.D., Eleanor, G., Francis, A.D., Alford, A.R., Storch, R.H. and Bentley, M.D. 1996. Citrus limonoid effects on Colorado potato beetle larval survival and development. Entomol. Exp. Appl., 80: 503-510.
Oliveira, M.R.V., Henneberry, T.J. and Anderson, P. 2001. History, current status, and collaborative research projects for Bemisia tabaci. Crop Protect., 20(9): 709-723.
Pedigo, L.P. 2002. Entomology and Pest Management, 4thedn. Iowa University Press. USA.
Robertson, J.L., Stansly, P.A. and Naranjo, S.E. 2014. Bemisia: Bionomics and Management of a Global Pest. J. Econ. Entomol., 107(1), 482-482.
Salminen, J.P. and Karonen, M. 2011. Chemical ecology of tannins and other phenolics: we need a change in approach. Funct. Ecol., 25(2): 325-338.
Shamsa, F., Monsef, H.R., Ghamooshi, R. and Verdian Rizi, M.R. 2007. Spectrophotometric Determination of Total Alkaloids in Peganum harmala L. Using Bromocresol Green. Res. J. Phytochem., 1 (2): 79-82.
Simmonds, M.S. 2001. Importance of flavonoids in insect–plant interactions: feeding and oviposition. Phytochem., 56(3): 245-252.
Walker, G.P. 2010. Life history, fanctional anatomy, feeding and mating behavior. In: Stansly, P,A, and Naranjo, S.E. (Eds) Bemisia: Binomics and management of global pest. Springer, Netherland. Pp: 109-160.
War, A.R., Paulraj, M.G., Ahmad, T., Buhroo, A.A., Hussain, B., Ignacimuthu, S. and Sharma, H.C. 2012. Mechanisms of plant defense against insect herbivores. Plant Sig. Behav., 7(10): 1306–1320
Yarahmadi, F. and Rajabpour, A. 2015. Organic fertilizer composition from Camellia sinensis extract for pest control and a method of synthesizing the same. U.S. Patent Application 14/733,970.
Yarahmadi, F., Rajabpour, A., Sohani, N.Z. and Ramezani, L. 2013. Investigating contact toxicity of Geranium and Artemisia essential oils on Bemisia tabaci Gen. Avicen. J. Phytomed., 3(2): 1-6.
Journal of Agricultural Science and Technology
Volume 23, Issue 3
May and June 2021
Pages 559-574