Improvement of Salt Tolerance and Growth in Common Bean (Phaseolus vulgaris L.) by Co-Inoculation with Native Rhizobial Strains

Authors
B. Khaitov 1
J. Vollmann 2
J. Yeong Pyon 3
K. W. Park 3
1 Department of Plant Science, Tashkent State Agrarian University, Uzbekistan.
2 Department of Crop Science, Vienna University of Natural Resources and Life Sciences, Austria.
3 Department of Crop Science, Chungnam National University, Korea.
Abstract
Beneficial association of rhizobial strains with leguminous plants may result in the enhancement of nodulation in the root and overall plant performance. In this study, the efficacy of inoculation was tested with pre-isolated Rhizobium phaseoli R9 and Mesorhizobium ciceri R6 as a single and in combined treatments on common bean (Phaseolus vulgaris L.) in a field experiment at soil salinity level of 5.6 dS m-1 over two growing seasons. The bacterial inoculations increased the plant height, root and shoot biomass, grain yield, number of nodules per plant, nodule dry weight, and root length of common bean, with a significant difference. Compared to single inoculation, co-inoculation of the rhizobial strains was more effective in all plant parameters and increased the seed yield by 35.1 and 37.9%, respectively, over the two consecutive seasons compared to the uninoculated control. Chemical analysis showed a significantly (P< 0.05) higher protein and oil content in the seeds of the co-inoculated plants than those of the control. The result of the experiment showed a strong correlation (r2= 0.87) between the increased nodule dry weight and seed yield of common bean. It might be concluded that co-inoculation with rhizobial strains could be the most effective biofertilization strategy for achieving greater nodulation and yield of common bean under saline conditions of Uzbekistan.

Keywords

Subjects

1. Araújo, S. S., Beebe, S., Crespi, M., Delbreil, B., Gonzalez, E. M., Gruber, V., Lejeune-Henaut, I., Link, W., Monteros, M. J., Prats, E., Rao, I., Vadez, V. and Patto, M. C. 2015. Abiotic Stress Responses in Legumes: Strategies Used to Cope with Environmental Challenges, Crit. Rev. Plant Sci., 34: 237-280.
2. Benidire, L., Lahrouni, M., El-Khalloufi, F., Göttfert, M. and Oufdou, K. 2017. Effects of Rhizobium leguminosarum Inoculation on Growth, Nitrogen Uptake and Mineral Assimilation in Vicia faba Plants under Salinity Stress. J. Agr. Sci. Tech. (JAST), 19(4): 889-901.
3. Beiranvand, J. P., Pourbabaee, A. A., Shirmardi, S. P., Alikhani, H. A., Abbasi, A. R. and Motesharezadeh, B. 2018. Symbiotic Nitrogen Fixation, Phosphorus and Potassium Uptake Capacity of a Number of Soybean Mutant Lines in a Calcareous Soil. J. Agr. Sci. Tech. (JAST), 20: 1555-1564.
4. Bertrand, A., Dhont, C., Bipfubusa, M., Chalifour, F. P., Drouin, P. and Beauchamp, C. J. 2015. Improving Salt-Stress Responses of the Symbiosis in Alfalfa Using Salt Tolerant Cultivar and Rhizobial Strain. Appl. Soil Ecol., 87: 108-117.
5. Bowen G. D. and Rovira, A. D. 1999. The Rhizosphere and Its Management to Improve Plant Growth. Adv. Agron., 1: 102.
6. Coyne, M. S., Mikkelsen, R. and Mineralization, S. 2015. Soil Microorganisms Contribute to Plant Nutrition and Root Health. Better Crops, 99: 18-20.
7. De Meyer, S. E., Van Hoorde, K., Vekemana, B., Braeckmana, T. and Willems, A. 2011. Genetic Diversity of Rhizobia Associated with Indigenous Legumes in Different Regions of Flanders (Belgium). Soil Biol. Biochem., 43: 2384-2396.
8. Figueiredo, M. V., Burity, H. A., Martínez, C. R. and Chanway, C. P. 2008. Alleviation of Drought Stress in the Common Bean (Phaseolus vulgaris L.) by Co-Inoculation with Paenibacillus polymyxa and Rhizobium tropici. Appl. Soil Ecol., 40(1): 182-188.
9. Goettsch, L., Lenssen, A., Yost, R., Luvaga, E., Semalulu, O., Tenywa, M. and Mazur, R. 2016. Improved Production Systems for Common Bean on Phaeozem Soil in South-Central Uganda. Afr. J. Agric. Res., 11(46): 4796-4809.
10. Goettsch, L. H., Lenssen, A.W., Yost, R. S., Luvaga, E. S., Semalulu, O., Tenywa, M., Miiro, R. and Mazur, R. E. 2017. Improved Production Systems for Common Bean on Ferralsol Soil in South-Central Uganda. Afr. J. Agric. Res., 12(23): 1959-1969.
11. Gopalakrishnan, S., Sathya, A., Vijayabharathi, R., Varshney, R. K., Gowda, C. L. and Krishnamurthy, L. 2015. Plant Growth Promoting Rhizobia: Challenges and Opportunities. 3 Biotech., 5(4): 355-377.
12. Hardarson, G., Bliss, F. A., Cigalesrivero, M. R., Henson, R. A., Kipenolt, J. A., Longeri, L., Manrique, A., Penacabriales, J. J., Pereira, P. A., Sanabria, C. A. and Tsai, S. M. 1993. Genotypic Variation in Biological Nitrogen-Fixation by Common Bean. Plant Soil, 152: 59-70.
13. Hungria, M., Campo, R. J. and Mendes, I. C. 2003. Benefits of Inoculation of the Common Bean (Phaseolus vulgaris) Crop with Efficient and Competitive Rhizobium tropici Strains. Biol. Fertil. Soil, 39(2): 88-93.
14. Hussain, M. B., Mehboob, I., Zahir, Z. A., Naveed, M. and Asghar, H. N. 2009. Potential of Rhizobium spp. for Improving Growth and Yield of Rice (Oryza sativa L.). Soil Environ., 28(1): 49-55.
15. Khaitov, B. 2016. Effect of Synergetic Interactions of Arbuscula micorrhiza Fungi and Rhizobia on Soybean Yield under Saline Condition. Genet. Plant Physiol., 6(3-4): 176-185.
16. Khaitov, B., Kurbonov, A., Abdiev, A. and Adilov, M. 2016. Effect of Chickpea in Association with Rhizobium to Crop Productivity and Soil Fertility. Eurasian J. Soil Sci., 5(2): 105-112.
17. Kienzler, K. M., Rudenko, I., Ruzimov, J., Ibragimov, N. and Lamers, J. P. A. 2011. Winter Wheat Quantity or Quality? Assessing Food Security in Uzbekistan. Food Secur., 3: 53–64.
18. Keneni, A., Prabu, P. C. and Assefa, F. 2010. Characterization of Acid and Salt Tolerant Rhizobial Strains Isolated from Faba Bean Fields of Wollo, Northern Ethiopia. J. Agr. Sci. Tech. (JAST), 12: 365-376.
19. Makoi, J. H., Bambara, S. and Ndakidemi, P. A. 2013. Rhizobium Inoculation and the Supply of Molybdenum and Lime Affect the Uptake of Macroelements in Common Bean (P. vulgaris L.) Plants. Aust. J. Crop Sci., 7: 784-794.
20. Massoud, O. N., Morsy, E. M. and El-Batanony, N. H. 2009. Field Response of Snap Bean (Phaseolus vulgaris L.) to N2-Fixers Bacillus circulans and Arbuscular Mycorrhizal Fungi Inoculation through Accelerating Rock Phosphate and Feldspar Weathering, Aust. J. Basic. Appl. Sci., 3: 844-852.
21. McConnell, M., Mamidi, S., Lee, R., Chikara, S., Rossi, M., Papa, R. and McClean, P. 2010. Syntenic Relationships among Legumes Revealed Using a Gene-Based Genetic Linkage Map of Common Bean (Phaseolus vulgaris L.). Theoretic. Appl. Genet., 121(6): 1103-1116.
22. Mehboob, I., Naveed, M., Zahir, Z.A. and Sessitch, A. 2013. Potential or rhizosphere bacteria for improving Rhizobium-Legume symbiosis. In: “Plant Microbe Symbiosis: Fundamentals and Advances”, (Ed.): Arora, N. K. Springer, India, PP. 305–349.
23. Mehboob, I., Zahir, Z. A., Arshad, M., Tanveer, A. and Azam, F. 2011. Growth Promoting Activities of Different Rhizobium spp. in Wheat. Pak. J. Bot., 43(3): 1643-1650.
24. Naseri, B., Fathipour, Y. and Talebi, A. A. 2009. Population Density and Spatial Distribution Pattern of Empoasca decipiens (Hemiptera: Cicadellidae) on Different Bean Species. J. Agr. Sci. Tech. (JAST), 11: 239-248.
25. Oliveira-Francesquini, J. P. D., Hungria, M., Savi, D. C., Glienke, C., Aluizio, R., Kava, V. and Galli-Terasawa, L. V. 2017. Differential Colonization by Bioprospected Rhizobial Bacteria Associated to Common Bean in Different Cropping Systems. Can. J. Microbiol., 63(8): 682-689.
26. Pineda, A., Zheng S. J., van Loon, J. J. A., Pieterse, C. M. J. and Dicke, M. 2010. Helping Plants to Deal with Insects: The Role of Beneficial Soilborne Microbes. Trends Plant Sci., 15: 507–514.
27. Pourbabaei, A. A., Bahmani, E., Alikhani, H. A. and Emami, S. 2016. Promotion of Wheat Growth under Salt Stress by Halotolerant Bacteria Containing ACC Deaminase. J. Agr. Sci. Tech. (JAST), 18(3): 855-864.
28. Qushimov, B., Ganiev, I. M., Rustamova, I., Haitov, B. and Islam, K.R. 2007. Land Degradation by Agricultural Activities in Central Asia. Climate Change and Terrestrial Carbon Sequestration in Central Asia, Taylor & Francis, London, PP. 137-147.
29. Rahmani, H. A., Räsänen, L. A., Afshari, M. and Lindström, K. 2011. Genetic Diversity and Symbiotic Effectiveness of Rhizobia Isolated from Root Nodules of Phaseolus vulgaris L. Grown in Soils of Iran. Appl. Soil Ecol., 48(3): 287-293.
30. Remans, R., Ramaekers, L., Schelkens, S., Hernandez, G., Garcia, A., Reyes, J. L., Mendez, N., Toscano, V., Mulling, M., Galvez, L. and Vanderleyden, J. 2008. Effect of Rhizobium-Azospirillum Coinoculation on Nitrogen Fixation and Yield of Two Contrasting Phaseolus vulgaris L. Cultivars Cultivated across Different Environments in Cuba. Plant Soil, 312: 25–37.
31. Saharan, B. and Nehra, V. 2011. Plant Growth Promoting Rhizobacteria: A Critical Review. Life Sci. Med. Res., 21: 1-30.
32. Riehm, H. 1985. Arbeitsvorschrift zur Bestimmung der Phosphorsäure und des Kaliums nach der Laktatmethode. Zeitschrift fur Pflanzen Düngung und Bodenkunde, 40: 152-156.
33. Stajković, O., Delić, D., Jošić, D., Kuzmanović, Đ., Rasulić, N. and Knežević-Vukčević, J. 2011. Improvement of Common Bean Growth by Co-Inoculation with Rhizobium and Plant Growth-Promoting Bacteria. Romanian Biotechnol. Lett., 16: 5919–5926.
34. Suárez, R., Wong, A., Ramírez, M., Barraza, A., Orozco, M. D. C., Cevallos, M. A., Lara, M., Hernández, G. and Iturriaga, G. 2008. Improvement of Drought Tolerance and Grain Yield in Common Bean by Overexpressing Trehalose-6-Phosphate Synthase in Rhizobia. Mol. Plant Microbe Interact., 21(7): 958-966.
35. Tejera, N.A., Soussi, M. and Lluch, C. 2006. Physiological and Nutritional Indicators of Tolerance to Salinity in Chickpea Plants Growing under Symbiotic Conditions. Environ. Exp. Bot., 58: 17-24.
36. Vakali, C., Baxevanos, D., Vlachostergios, D., Tamoutsidis, E., Papathanasiou, F. and Papadopoulos, I. 2017. Genetic Characterization of Agronomic, Physiochemical, and Quality Parameters of Dry Bean Landraces under Low-Input Farming. J. Agr. Sci. Tech. (JAST), 19(4): 757-767.
37. Vargas, M. A. T., Mendes, I. C. and Hungria, M. 2000. The Response of Field Grown Phaseolus vulgaris to Rhizobium Inoculation and Nitrogen Fertilization in Two Cerrado Soils. Biol. Fertil. Soil, 32: 228–233.
38. FAO 2016. www.fao.org/faostat/en/
39. Yadegari, M. and Asadi Rahmani, H. 2010. Evaluation of Bean (Phaseolus vulgaris) Seeds’ Inoculation with Rhizobium Phaseoli and Plant Growth Promoting Rhizobacteria (PGPR) on Yield and Yield Components. Afr. J. Agric. Res., 5: 792–799.
Journal of Agricultural Science and Technology
Volume 22, Issue 1
January and February 2020
Pages 209-220