Controlling Secondary Somatic Embryogenesis in Persian Oak (Quercus brantii L.) Using Hormonal Compounds and Media

Authors
E. Faizy
A. Moradi
A. Masoumi Asl
Department of Agronomy and Plant Breeding, College of Agriculture, Yasouj University, Yasouj, Islamic Republic of Iran.
Abstract
Using immature embryos that undergo somatic embryogenesis, we studied the effects of different hormonal compounds and media on controlling secondary somatic embryogenesis (SSE) in Persian oak (Quercus brantii L.). To this end, we focused on the immature embryos that were subjected to several treatments including chilling (at 4 ℃) period and SH, MS, 2,4-D, IBA, BAP, and glutamine concentrations in 5 separate sequential experiments. The results showed that, by extending chilling period to 8-weeks, SSE induction was reduced (68.75%). In different MS concentrations or MS containing PGRs, the lowest globular embryo (66%) and the secondary embryo induction (87.5%) were observed in embryos treated with MS+IBA+BA. Adding 0.75 mg L-1 glutamine to MS resulted in a decrease in the secondary somatic embryogenesis (56.25%). Among MS and SH media, 1/2 SH almost entirely controlled this phenomenon (6.25%). The highest maturation progression was obtained in the SH+glutamine treatment, which had the highest conversion to plantlet percentage (100%) and vigor index of plantlets (51.93) compared to the use of SH alone. We found that nutrient and PGR concentration were critical in embryo maturation and conversion percentage and stop the embryo induction cycle that plays a major role in secondary embryogenesis.

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1. Abdul-baki, A. A. and Anderson, J. D. 1970. Viability and Leaching of Sugars from Germinating Barely. Crop Sci., 10: 31-34.
2. Agarwal, S., Kanwar, K. and Sharma, D. R. 2004. Factors Affecting Secondary Somatic Embryogenesis and Embryo Maturation in Morus alba L. Sci. Hort., 102: 359–368.
3. Aldrich, P. R. and Cavender-Bares, J. 2011. Quercus. In: Kole, C. (Ed.) Wild Crop Relatives: Genomic and Breeding Resources, Forest Trees. Springer-Verlag, Berlin, pp. 89-129.
4. Barra-Jimenez, A., Blasco, M. and Ruiz-Galea, M. 2014. Cloning Mature Holm Oak Trees by Somatic Embryogenesis. Trees., 28:657-667.
5. Bewley, J., Bradford, K., Hilhorst, H. and Nonogaki, H. 2013. Physiology of Development, Germination, and Dormancy. 3rd Edition, 394 pp.
6. Bueno M. A., Astorga R. and Manzanera, J.A. 1992. Plant Regeneration through Somatic Embryogenesis in Quercus suber. J. Phy. Pl., 85:30-34.
7. Bueno, M. A., Gómez, A. and Manzanera, J.A. 2000. Somatic and Gametic Embryogenesis in Quercus suber L. In: Jain SM, Gupta PK, Newton RJ (Eds.) Somatic Embryogenesis in Woody Plants. Kluwer Academic Publishers. 6: 479-508.
8. Burbulis, N., Kupriene, R. and Liakas, V. 2007. Somatic Embryogenesis and Plant Regeneration in Immature Zygotic Embryos of Brassica napus. Acta Universitais Latviensis. 723: 27-35.
9. Calic, D., Zdravkovic-korac, S. and Radojevic, L. 2005. Secondary Embryogenesis in Androgenic Embryo Cultures of Aesculus hippocastanum L. Biol. Plant., 49 (3): 435-438.
10. Das, P., Rout, G. R. and Das, A. B. 1993. Somatic Embryogenesis in Callus Culture of Mussaenda erythrophylla cvs, Queen sirikit and Rosa. Pl. Cell Tiss. Org. Cult., 35: 199-201.
11. Deng, M. D. and Comu, D. 1992. Maturation and Germination of Walnut Somatic Embryos. Pl. Cell Tiss. Org. Cult., 28:195-202.
12. El Maataoui, M., Espagnac, H. and Michaux-Ferritre, N. 1990. Histology of Callogenesis and Somatic Embryogenesis Induced in Stem Fragments of Cork Oak (Quercus suber) Cultured in Vitro. Ann. Bot., 66:183-190.
13. Fernandez-Guijarro, B., Celestino, C. and Toribio, M. 1994. Influence of External Factors on Secondary Embryogenesis and Germination in Somatic Embryos from Leaves of Quercus suber. Plant Cell Tiss. Org. Cult., 41: 99-106.
14. Finer, J. J., Kriebel, H. B. and Beewar, M. R. 1989. Initition of Embryogenic Callus and Suspension Cultures of Eastern White Pine (Pinus strobus L.). Plant Cell Rep., 8: 203-206.
15. Gingas, V. M. 1991. Asexual Embryogenesis and Plant Regeneration from Male Catkins of Quercus. J. Hort. Sci., 26:1217-1218.
16. Gingas, V. M. and Lineberger, R. D. 1989. Asexual Embryogenesis and Plant Regeneration in Quercus sp. Plant Cell, Tiss. Org. Cult., 17: 191-203.
17. Gupta, P. K. and Durzan, D. J. 1991. Loblolly Pine (Pinus taeda ( L.). In: “Biotechnology in Agriculture and Forestry, Trees III” (ed): Bajaj, Y. P. S., Springer-Verlag, Berlin., 16: 383-407.
18. Jefferson, L. V. and Penachchio, M. 2003. Allelopathic Effects of Foliage Extracts from Four Chenopodiaceae Species on Seed Germination, J. Arid Enviro., 55: 275-285.
19. Kim, Y. J., Lee, O. R., Kim, K. T. and Yang, D. C. 2012. High Frequency of Plant Regeneration through Cyclic Secondary Somatic Embryogenesis in Panax ginseng. J Ginseng Res. 36 (4): 442-448.
20. Mallon, R., Purificacion, C. and Vieitez, A. M. 2012. Improving Secondary Embryogenesis in Quercus robur: Application of Temporary Immersion for Mass Propagation. Trees., 26:731-741.
21. Martinez, M. T., Ballester, A., Vieitez, A. and Corredoira, E. 2014. Induction of Somatic Embryogenesis in Leaf and Shoot Apex Explants Derived from Red Oak Trees: Effects of Explant Type, Silver Thiosulphate and Activated Charcoal on the Embryogenic System. Proceedings of the 3rd International Conference of the IUFRO Unit 2.09.02 on "Woody Plant Production Integrating Genetic and Vegetative Propagation Technologies", Vitoria-Gasteiz, Spain, 8-12 September pp.58-65.
22. Mauri, P. V. and Manzanera, J. A. 2003. Induction, Maturation, and Germination of Holm Oak (Quercus ilex L.) Somatic Embryos. Plant Cell, Tiss. Org. Cult., 74: 229-235.
23. Mohan Jainne, S. and Haggman, H. 2007. Protocols for Micropropagation of Woody Trees and Fruits. Springer Verlag, Pp. 558.
24. Naouar, B. A. and Lamarti, A. 2014. Macronutrients Effect on Secondary Somatic Embryogenesis of Moroccan Cork Oak (Quercus suber L.). American J. Pl. Sci., 5: 1851-1861.
25. Naing, A. H., Kim, C. K., Yun, B. J. and Lim, K. B. 2013. Primary and Secondary Somatic Embryogenesis in Chrysanthemum cv. Euro. Plant Cell Tiss., Organ Cult. 112: 361-368.
26. Pintos, B., Bueno, M. A., Cuenca, B. and Manzanera, J. A. 2008. Synthetic Seed Production from Encapsulated Somatic Embryos of Cork Oak (Quercus suber L.) and Automated Growth Monitoring. Plant Cell Tiss. Org. Cult., 95(2): 217-225.
27. Pintos, B., Manzanera, J. A. and Angeles Bueno, M. 2009. Oak Somatic and Gametic Embryos Maturation Is Affected by Charcoal and Specific Amino Acids Mixture. INRA, EDP Sciences. Ann. For. Sci., 67 (2010) 205.
28. Pintos, B., Sánchez, N., Bueno, M. A., Navarro, R. M., Jorrín, J., Manzanera, J. A. and Gómez-Garay, A. 2013. Induction of Quercus ilex L. Haploid and Doubled-Haploid Embryos from Anther Cultures by Temperature-stress. Sil. Gen., 62: 210-218.
29. Raemakers, C. J. J. M., Jacobsen, E. and Visser, R. G. F. 1994. Secondary Somatic Embryogenesis and Applications in Plant Breeding. Kluwer Academic Publishers., 81: 93-107.
30. Rai, M. K., Jaiswal V. S., and Jaiswal U., 2009. Effect of Selected Amino Acids and Polyethylene Glycol on Maturation and Germination of Somatic Embryos of Guava (Psidium guajava L.). Sci. Hort., 121: 233-236.
31. Robichaud, R. L., Lessard, V. C., Merkle, S. A., 2004. Treatments Affecting Maturation and Germination of American Chestnut Somatic Embryos. J. Pl. Phy. 161: 957-969.
32. Shi, X., D. Xigang, L. Guofeng, Zh. Junwei, N. Guogui, B. Manzhu, 2010. Cyclic Secondary Somatic Embryogenesis and Efficient Plant Regeneration in Camphor Tree (Cinnamomum camphora L.). In Vitro Cell. Dev. Biol. Plant. 46:117-125.
33. Solis Ramos, L. Y. 2012. Somatic Embryogenesis in Recalcitrant Plants. In: “Embryogenesis” (ed): Ken-Ichi S. In Tech, Rijeka., pp. 597–618.
34. Toribio, M. and Celestino, C. 1989. Cultivo in vitro Del alcornoque. Sci. Gerun., 15: 11-21.
35. Toribio, M., Fernandez, C., Celestino, C., Martınez, M. T., San-Jose M. C. and Vieitez, A. M. 2004. Somatic Embryogenesis in Mature Quercus robur Trees. Plant Cell Tiss. Org. Cult., 76: 283-287.
36. Valizadeh, M. and Kazemi Tabar, S. K. 2009. Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch. J. Agr. Sci. Tech., 11: 481-486
37. Vasic D., Alibert G., Skoric D. 2001. Protocols for Efficient Repetitive and Secondary Somatic Embryogenesis in Helianthus maximiliani (Schrader). Pl. Cell Rep. 20: 121-125.
38. Vengadesan, G. and Paula Pijut, M. 2009. Somatic Embryogenesis and Plant Regeneration on Northern Red Oak (Quercus rubra L.). Plant Cell Tiss. Org. Cult., 97:141-149
39. Vonarnold, S., Egertddostter, U. and Mo, L. H. 1994. Importance of Extracellular Proteins for Somatic Embryogenesis in Picea abies. Curr. Issu. Plant Mol. Cell. Biol., 22: 389-392.
40. Yang, J., S. Wu, and Ch. Li, 2013. High-Efficiency Secondary Somatic Embryogenesis in Hovenia dulcis Thunb. Through Solid and Liquid Cultures. Hindawi Publishing Corporation the ScientificWorld J., Article ID 718754, 6 pages. 1-6.
41. Zhou, S. and Brown, D. C. W. 2006 High Efficiency Plant Production of North American Ginseng Via Somatic Embryogenesis from Cotyledon Explants. Plant Cell Rep. 25: 166-173.
42. Zolfaghari R. 2009. Study of Drouth Resistant in Persian oak Plantlet (Quercus brantii Lindl.) Using Morphological, Physiological, Biochemical and Molecular Markers. Forestry Doctoral Thesis, Natural Resources College, and Marine Sciences, Tarbiat Modarres University, Tehran.
43. Zouine, J., Hadrami, I. E. I., 2007. Effect of 2,4-D, Glutamine, and BAP on Embryogenic Suspension Culture of Date Palm (Phoenix dactylifera L.). Sci. Hort. 112: 221-226.
Journal of Agricultural Science and Technology
Volume 21, Issue 3
May and June 2019
Pages 615-626