Methyl Jasmonate Mitigates Drought Stress Injuries and Affects Essential Oil of Summer Savory

Authors
1 Department of Horticultural Sciences, College of Agriculture, Ilam University, Ilam, Islamic Republic of Iran.
2 Department of Horticultural Sciences, College of Agriculture, Bu-Ali Sina University, Hamedan, Islamic Republic of Iran.
Abstract
Drought stress reduces growth and productivity of crops in arid and semiarid regions, such as Iran. Methyl Jasmonate (MeJA) is involved in regulating plants growth and their immune responses. The main purpose of this study was to evaluate the effects of MeJA on growth and some physiological responses of summer savory (Satureja hortensis) plant under drought stress condition. Treatments consisted of three levels of drought stress including stress free conditions, mild stress and severe stress, and four concentrations of MeJA (0, 75, 150, and 225 μM). The results showed that drought stress and MeJA application had a significant effect on morphological, physiological, and biochemical parameters. In drought conditions, some growth parameters, namely, relative water content and essential oil yield decreased and antioxidant activity, proline content, and essential oil percentage increased. The results suggest that MeJA application under drought stress can improve growth parameters as well as relative water content, proline content, antioxidant activity, and essential oil percentage and yield. The best protection appeared to be obtained from plants treated with MeJA at 75 μM.

Keywords


1. Agrawal, G. K., Tamogamib, S., Iwahashic, H., Agrawala, V. P. and Rakwal, R. 2003. Transient Regulation of Jasmonic Acid Inducible Rice MAPkinase Gene (OsBWMK1) by Diverse Biotic and Abiotic Stresses. Plant. Physiol. Bioch., 41: 355–361.
2. Aliabadi Farahani, H., Arbab, A. and Abbaszadeh, B. 2008. The Effects of Super Phosphate Triple, Water Deficit Stress and Glomus hoi Biological Fertilizer on Some Quantity and Quality Characteristics of Coriandrum sativum L. Iran. J. Med. Arom. Plant., 24: 18-30.
3. Alishah, O. and Ahmadikhah, A. 2009. The Effects of Drought Stress on Improved Cotton Varieties in Golesatn Province of Iran. Int. J. Plant. Prod., 3: 17-26.
4. Anjum, S. A., Wang, L., Farooq, M., Khan, I. and Xue, L. 2011. Methyl Jasmonate-induced Alteration in Lipid Peroxidation, Antipxidative Defense System and Yield in Soybeans under Drought. Afr. J. Biotechnol., 197: 296-301.
5. Bahreininejad, B., Razmjoo, J. and Mirza, M. 2013. Influence of Water Stress on Morpho-Physiological and Phytochemical Traits in Thymus daenensis. Int. J. Plant. Prod., 7: 151-166.
6. Battes, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid Determination of Free Proline for Water Stress Studies. Plant Soil, 29: 205-207.
7. Bor, M., Ozdemir, F. and Turkan, I. 2003. The Effect of Salt Stress on Lipid Peroxidation and Antioxidants in Leaves of Sugar Beet Beta vulgaris L. and Wild Beet Beta maritima L. Plant Sci., 164: 77–84.
8. Burbott, A. J. and Loomis, D. 1969. Evidence for Metabolic Turnover Monoterpenes in Pepper Mint. Plant Physiol., 44: 173–179.
9. Cheong, J. J. and Choi, Y. D. 2003. Methyl Jasmonate as a Vital Substance in Plants. Trends. Genet, 19: 409-413.
10. Creelman, R. and Mullet, J. E. 1995. Jasmonic Acid Distribution and Action in Plants: Regulation during Development and Response to Biotic and Abiotic Stress. Proc. Natl. Acad. Sci., 92: 4114-4119.
11. Ghasem, F., Poustini, K., Besharati, F., Mohammadi, H. V. A., Abooei Mehrizi, F. and Goettfert, M. 2012. Pre-incubation of Sinorhizobium meliloti with Luteolin, Methyl jasmonate and Genistein Affecting Alfalfa (Medicago sativa L.) Growth, Nodulation and Nitrogen Fixation under Salt Stress Conditions. J. Agr. Sci. Tech., 14: 1255-1264.
12. Gundlach, H., Muller, M. J., Kutchan, T. M. and Zenk, M. H. 1992. Jasmonic Acid is a Signal Transducer in Elicit or Induced Plant Cell Cultures. Proc. Natl. Acad. Sci., 89: 2389–2393.
13. Hassanein, R. A., Hassanein, A. A., El-Din, A. B., Salama, M. and Hashem, H. A. 2009. Role of Jasmonic Acid and Abscisic Acid Treatments in Alleviating the Adverse Effects of Drought Stress and Regulating Trypsin Inhibitor Production in Soybean Plant. Aust. J. Basic. Appl. Sci., 3: 904-919.
14. Huang, Y. C., Chang, Y. H. and Shao, Y. Y. 2006. Effects of Genotype and Treatment on the Antioxidant Activity of Sweet Potato in Taiwan. Food Chem., 98: 529–538.
15. Huiling, W. U., Xiaoli, W. U., Zhaohu, L., Liusheng, D. and Mingcai, Z. H. 2012. Physiological Evaluation of Drought Stress Tolerance and Recovery in Cauliflower (Brassica oleracea L.) Seedlings Treated with Methyl Jasmonate and Coronatine. J. Plant. Growth. Regul., 31: 113–123.
16. Inze, D. M. and Montage, V. 1995. Oxidative Stress in Plant. J. Biotechnol., 6: 153-159.
17. Khazaiea, H. R., Nadjafib, F. and Bannayan, M. 2008. Effect of Irrigation Frequency and Planting Density on Herbage Biomass and Oil Production of Thyme (Thymus vulgaris) and Hyssop (Hyssopus officinalis). Indus. Crop. Prod., 27: 315–321.
18. Korkmaz, A., Korkmaz, Y. and Demirkiran, A. R. 2010. Enhancing Chilling Stress Tolerance of Pepper Seedling by Exogenous Application of 5-Aminolevolinic Acid. J. Envi. Exp. Bot., 67: 495-501.
19. Larson, R. A. 1988. The Antioxidants of Higher Plants. Phytochem., 27: 969-78.
20. Mahmood, M., Shirani Bidabadi, S., Ghobadi, C. and Gray, D. 2012. Effect of Methyl Jasmonate Treatment on Alleviation of Polyethylene Glycolmediated Water Stress in Banana (Musa acuminate cv. Berangan) Shoot Tip Cultures. Plant Growth Regul., 68: 161-169.
21. Mohamet, M. and Abdu, M. 2004. Growth and Oil Production of Fennel (Foeniculum vulgar Mill): Effect of Irrigation and Organic Fertilization. Biol. Agri. Hort., 22: 31-39.
22. Moon, J. H. and J. Terao. 1998. Antioxidant Activity of Caffeic Acid and Dihydrocaffeic Acid in Lard and Low-density Lipoprotein. J. Agric. Food Chem., 46: 5062-5065.
23. Nautiyal, P. C., Rachaputi, N. R. and Joshi, Y. C. 2002. Moisture-Deficit-Induced Changes in Leaf Water Content, Leaf Carbon Exchange Rate and Biomass Production in Groundnut Cultivars Differing in Specific Leaf Area. Field Crop. Res., 74: 67-69.
24. Oh, M. M., Trick, H. N. and Rajashekar, C. B. 2009. Secondary Metabolism and Antioxidants Are Involved in Environmental Adaptation and Stress Tolerance in Lettuce. J. Plant. Physiol., 166: 180–191.
25. Price, A. H., Cairns, J. E., Horton, P., Jones, H. G. and Griffiths, H. 2002. Linking Drought-Resistance Mechanisms to Drought avoidance in Upland Rice Using a QTL Approach: Progress and New Opportunities to Integrate Stomatal and Mesophyll Responses. J. Envi. Exp. Bot., 53: 989–1004.
26. Redy, T. Y., Reddy, V. R. and Anbumozhi, V. 2003. Physiological Responses of Groundnut (Arachis hypogea L.) to Drought Stress and Its Amelioration a Critical Review. Plant Growth Regul., 41: 75-88.
27. Robertson, M. J. and Holland, J. F. 2004. Production Risk of Canola in the Semiarid Subtropics of Australia. Aust. J. Agri. Res., 55: 525– 538.
28. Rohwer, C. L. and Erwin, J. E. 2008. Horticultural Applications of Jasmonates: A Review. J. Hort. Sci. Bio. Technol., 83: 283-304.
29. Rosegrant, M. W. and Cline, S. A. 2003. Global Food Security: Challenges and Policies. Sci., 302: 1917–1919.
30. Shan, C. J. and Liang, Z. S. 2010. Jasmonic Acid Regulates Ascorbate and Glutathione Metabolism in Agropyron cristatum Leaves under Water Stress. Plant Sci., 178: 130–139.
31. Shao, H., Chu, L., Jaleel, C. A. and Zhao, C. 2008. Water-deficit Stress-induced Anatomical Changes in Higher Plants. C. R. Biologes, 331: 215-225.
32. Sharafzadeh, S. H. 2011. Influence of Growth Regulators on Growth and Secondary Metabolites of Some Medicinal Plants from Lamiaceae Family. Adv. Environ. Biol., 5: 2296-2302.
33. Simon, J. E., Bubenheim, R. D., Joly, R. J. and Charles, D. J. 1992. Water Stress Induced Alternations in Essential Oil Content and Composition of Sweet Basil. J. Essent. Oil Res., 4: 71–75.
34. Singh, M., Ganesha-Rao, R. S. and Ramesh, S. 1997. Irrigation and Nitrogen Requirement of Lemongrass (Cymbopogon flexuosus (Sleud) Wats) on a Red Sandy Loam Soil Under Semiarid Tropical Conditions. J. Essent. Oil Res., 9: 569-574.
35. Sylviane, M., Comparot, C., Graham, M. and Reid, M. D. 2002. Methyl Jasmonate Elicits a Differential Antioxidant Response in Light- and Dark-grown Canola (Brassica napus) Roots and Shoots. Plant. Growth Regul, 38: 21–30.
36. Tarumingkeng, R. C. and Coto, Z. 2003. Effects of Drought Stress on Growth and Yield of Soybean. Kisman. Sci. Philosopy, 702.
37. Van der Fits, L. and Memelink, J. 2000. ORCA3, a Jasmonate Responsive Transcriptional Regulator of Plant Primary and Secondary Metabolism. Sci., 289: 295-297.
38. Yazdanpanah, S., Baghizade, A. and Abbasi, S. 2011. The Interaction between Drought Stress and Salicylic Acid and Ascorbic Acid on Some Biological Characteristics of Satureja hortensis. Afr. J. Agri. Res., 6: 798-807.
39. Zalewski, K., Nitkiewicz, B., Lahuta, L. B., Głowacka, K., Socha, A. and Amarowicz, R. 2010. Effect of Jasmonic Acid Methyl Ester on the Composition of Carbohydrates and Germination of Yellow Lupine (Lupinus luteus L.) Seeds. J. Plant Physiol., 167: 967–973.