Development and Characterization of Fermented and Unfermented Whey Beverages Fortified with Vitamin E

Authors
Z. Sohrabi 1
M. H. Eftekhari 2
M. H. Eskandari 3
A. Rezaeianzadeh 4
M. M. Sagheb 5
1 Department of Community Nutrition, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
2 Department of Clinical Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
3 Department of Food Science and Technology Epidemiology, School of Agriculture, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
4 Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
5 Department of Nephrology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Islamic Republic of Iran.
Abstract
Regarding hemodialysis patients’ limitations in consuming different foods, providing them with a product with health-promoting effects is important. The objective of this study was to develop a functional food from whey protein for this disease condition and assess its sensory, chemical, and nutritional characteristics. Fermented and unfermented beverages were prepared as a mixture of whey protein concentrate (8.5%), permeate (1.4 % for fermented), mint flavor (0.01%), vitamin E (0.18%), and water. Ty17A starter was used for the fermented blend. After pasteurization, and homogenization, the blends were stored until the sensory, chemical, and nutritional evaluations were done. Data were analyzed by SPSS Software (version 16). The pH values of the fermented and unfermented beverages were 3.8 and 6.02, respectively. The medians of all sensory attributes were greater than 4 for the fermented and less than 3 for the unfermented beverages (except color). All sensory attributes other than the color were significantly different between beverages (P< 0.05). The overall acceptability of the unfermented beverage was less. No significant nutritional differences were seen between beverages. Some of the nutrients values in the beverages are desirable for hemodialysis patients (Protein: 7.9-8 g 100 g-1, Fat: 0.4%, Trans fatty acids: 2%, Saturated fatty acids: 56.15% Phosphorus: 9.25-9.35 mg 100 g-1, Potassium: 0.0295 %, Sodium: 62.5 mg 100 g-1, and vitamin E: 400 mg 220 mL-1). The microbial counts of both were safe. Vitamin E fortified fermented whey beverage might be a good recommendation for hemodialysis patients because of its protein quality, low fat, phosphorus, sodium, and potassium and high vitamin E contents.

Keywords

1. Aguilera, A., Bajo, M. A., Del Peso, G., Diez, J. J., Codoceo, R., Rebollo, F. Mariano, M. and Selgas, R. 2002. True Deficiency of Antioxidant Vitamins E and A in Dialysis Patients. Relationship with Clinical Patterns of Atherosclerosis. Adv. Perit. Dial.,18: 206-11.
2. Ahmadi, A., Mazooji, N., Roozbeh, J., Mazloom, Z. and Hasanzade, J. 2013. Effect of Alpha-lipoic Acid and Vitamin E Supplementation on Oxidative Stress, Inflammation, and Malnutrition in Hemodialysis Patients. Iran. J. Kidney Dis., 7(6): 461-7.
3. Association of Official Analytical Chemists (AOAC). 2002. Official Methods of Analysis. Milk and Milk Products (Yoghurt): Specifications and Test Methods. Number 695 2002, AOAC Gaithersburg, USA.
4. Association of Official Analytical Chemists (AOAC). 1999. Official Methods of Analysis. Nitrogen (Total) in Milk: Kjldahl Method. 5th Revision, 16th Edition, Number 695 2002, Gaithersburg, USA.
5. Association of Official Analytical Chemists (AOAC). 1999. Official Methods of Analysis. Sodium and Potassium in Dried Milk: Flame Emission Spectrometric Method. 5th Revision, 16th Edition, Number 695 2002, AOAC, Gaithersburg, USA.
6. Association of Official Analytical Chemists (AOAC). 2002. Official Methods of Analysis. Phosphorus (Total) in Foods: Calorimetric Methods. 5th Revision, 16th Edition, Number 695 1999, AOAC, Gaithersburg, USA.
7. Ballard, K. D., Kupchak, B. R., Volk, B. M., Mah, E., Shkreta, A., Liptak, C., Ptolemy, A. S., Kellogg, M. S., Bruno, R. S., Seip, R.L., Maresh, C. M., Kraemer, W. J. and Volek, J. S. 2013. Acute Effects of Ingestion of a Novel Whey-derived Extract on Vascular Endothelial Function in Overweight, Middle-Aged Men and Women. Br. J. Nutr., 109(5): 882-93.
8. Butteiger, D. N., Cope. M., Liu, P., Mukherjea, R., Volpi, E., Rasmussen, B. B. and Krul, E. S. 2013. A Soy, Whey and Caseinate Blend Extends Postprandial Skeletal Muscle Protein Synthesis in Rats. J. Clin. Nutr., 32(4): 585-91.
9. Cote, A., Brown, W. A., Cameron, D. and Van Walsum, G. P. 2004. Hydrolysis of Lactose in Whey Permeate for Subsequent Fermentation to Ethanol. J. Dairy Sci., 87(6).
10. Daud, Z. A., Tubie, B., Adams, J., Quainton, T., Osia, R., Tubie, S. Kaur, D., Khosla, P. and Sheyman, M. 2012. Effects of Protein and Omega-3 Supplementation, Provided during Regular Dialysis Sessions, on Nutritional and Inflammatory Indices in Hemodialysis Patients. Vasc. Health Risk Manag., 8: 187-95.
11. Daud, Z. A., Tubie, B., Sheyman, M., Osia, R., Adams, J., Tubie, S. and Khosla, P. 2013. Vitamin E Tocotrienol Supplementation Improves Lipid Profiles in Chronic Hemodialysis Patients. Vasc. Health Risk Manag., 9: 747-61.
12. Evans, J., Zulewska, J., Newbold, M., Drake, M. A. and Barbano, D. M. 2010. Comparison of Composition and Sensory Properties of 80% Whey Protein and Milk Serum Protein Concentrates. J. Dairy Sci., 93(5): 1824-43.
13. Forsum, E. and Hambraeus, L. 1977. Nutritional and Biochemical Studies of Whey Products. J. Dairy Sci., 60(3): 370-7.
14. Gallagher, M. 2008. The Nutrients and Their Metabolism. In: "Krause's Food and Nutrition Therapy", (Eds.): Mahan, L. K. and Escott-Stump, S.. International Edition, Canada, PP. 51-109.
15. Gallardo-Escamilla, F. J., Kelly, A. L. and Delahunty, C. M. 2007. Mouthfeel and Flavour of Fermented Whey with Added Hydrocolloids. Int. Dairy J., 17(4): 308-15.
16. Jha, V., Garcia, G., Iseki, K., Li, Z., Naicker, S., Plattner, B., Saran, R., Wang, A. Y. and Yang, C. W. 2013. Chronic Kidney Disease: Global Dimension and Perspectives. Lancet., 382(9888): 260-72.
17. Gürgen, S., Yücel, A., Karakuş, A., Ceçen, D., Ozen, G. and Koçtürk, S. 2014. Usage of Whey Protein May Cause Liver Damage via Inflammatory and Apoptotic Responses. Hum. Exp. Toxicol., Epub ahead of print.
18. Hering, N., Andres, S., Fromm, A., Tol, E., Amashesh, M., Mankertz, J., Fromm, M. and Schulzke, J D. 2011. Transforming Growth Factor-β, a Whey Protein Component, Strenghtens the Intestinal Barrier by Upregulating Claudin-4 in HT-29/B6 Cells. J. Nutr., 141(5): 783-8.
19. House, S. D. 1997. Determination of Total, Saturated, and Monounsaturated Fats in Foodstuffs by Hydrolytic Extraction and Gas Chromatographic Quantitation: Collaborative Study. J. AOAC Int., 80(3): 555-63.
20. Jinjarak, S., Olabi, A., Jimenez-Flores, R., Sodini, I. and Walker, J. H. 2006. Sensory Evaluation of Whey and Sweet Cream Buttermilk. J. Dairy Sci., 89(7): 2441-50.
21. Kalantar-Zadeh, K., Ikizler, T. A., Block, G., Avram, M. M. and Kopple, J. D. 2003. Malnutrition-inflammation Complex Syndrome in Dialysis Patients: Causes and Consequences. Am. J. Kidney Dis., 42(5): 864-81.
22. Kunz, C. and Lonnerdal, B. 1990. Human-milk Proteins: Analysis of Casein and Casein Subunits by Anion-exchange Chromatography, Gel Electrophoresis, and Specific Staining Methods. Am. J. Clin. Nutr., 51(1): 37-46.
23. Mahan, L. K. and Escott-Stump, S. 2008. Krause's Food and Nutrition Therapy (Appendix 34). 12th Edition, International Edition, What kind of publication.
24. Pakpour, A. H., Nourozi, S., Molsted, S., Harrison, A. P., Nourozi, K. and Fridlund, B. 2011. Validity and Reliability of Short Form-12 Questionnaire in Iranian Hemodialysis Patients. Iran. J. Kiney Dis., 5(3): 175-81.
25. Pescuma, M., Hébert , E. M., Mozzi, F. and De Valdez, G. F. 2010. Functional Fermented Whey-Based Beverage Using Lactic Acid Bacteria. Int. J. Food Microbiol., 146(1-2): 73-81.
26. Pollock, J. B. and Jaffery, J. B. 2007. Knowledge of Phosphorus Compared with Other Nutrients in Maintenance Dialysis Patients. J. Ren. Nutr., 17(5): 323-8.
27. Putri, A. Y. and Thaha, M. 2014. Role of Oxidative Stress on Chronic Kidney Disease Progression. Acta Med. Indones., 46(3): 244-52.
28. Rusu, A., Rusu, F., Zalutchi, D., Muresan, A., Gherman Caprioara, M. and Kacso, I. 2013. The Influence of Vitamin E Supplementation on Erythropoietin Responsiveness in Chronic Hemodialysis Patients with Low Levels of Erythrocyte Superoxide Sismutase. Int. Urol. Nephrol., 45(2): 495-501.
29. Salehi, M., Sohrabi, Z., Ekramzadeh, M., Fallahzadeh, M. K., Ayatollahi, M., Geramizadeh, B., Hassanzadeh, J. and Sagheb, M. M. 2013. Selenium Supplementation Improves the Nutritional Status of Hemodialysis Patients: A Randomized, Double-blind, Placebo-controlled Trial. Nephrol. Dial. Transplant., 28(3): 716-23.
30. Savica, V., Calo, L. A., Monardo, P., Caldarera, R., Cavaleri, A., Santoro, D., Muraca, U., Mallamace, A. and Bellinghieri, G. 2008. High Phosphate Content Beverages in Dialysis Patients: Relevance for Hyperphosphatemia and Cardiovascular Risk. Nutr. Metab. Cardiovasc. Dis., 18(8): e39-40.
31. Siefker, K. and Disilvestro, R. A. 2006. Safety and Antioxidant Effects of a Modest Soy Protein Intervention in Hemodialysis Patients. J. Med. Food, 9(3): 368-72.
32. Sugawara, K., Takahashi, H., Kashiwagura, T., Yamada, K., Yanagida, S., Homma, M., et al. Please add all of the authors names!!! 2012. Effect of Anti-inflammatory Supplementation with Whey Peptide and Exercise Therapy in Patients with COPD. Respir. Med., 106(11): 1526-34.
33. Sukar, S. G., Gallo, F., Borrini, C., Vaccaro, A., Marchello, C., Boicelli, R., Borgarelli, C., Solari, P., Ratto, C. E. and Ravera, G. 2012. Effects of a New Mixture of Essential Amino Acids (Aminotrofic(R)) in Malnourished Haemodialysis Patients. Med. J. Nutrition Metab., 5(3): 259-66.
34. Sundell, M. B., Cavanaugh, K. L., Wu, P., Shintani, A., Hakim, R. M. and Ikizler, T. A. 2009. Oral Protein Supplementation Alone Improves Anabolism in a Dose-dependent Manner in Chronic Hemodialysis Patients. J. Ren. Nutr., 19(5): 412-21.
35. Toedebusch, R. G., Childs, T. E., Hamilton, S. R., Crowley, J.R., Booth, F. W. and Roberts, M. D. 2012. Postprandial Leucin and Insulin Responses and Toxicological Effects of a Novel Whey Protein Hydrolysate-based Supplements in Rats. J. Int. Soc. Sport. Nutr., 9(24): 2-10.
36. Vidal-Valverde, C., Martin-Villa, C. and Herranz, J. 1984. Determination of Soluble Carbohydrates in Yogurts by High Performance Liquid Chromatography. J. Dairy Sci., 67(4): 759-63.
37. Willkens, K. and Juneja, V. 2008. Medical Nutrition Therapy for Renal Disorders. In: "Krause's Food and Nutrition Therapy", (Eds.): Mahan, L. K. and Escott-Stump, S.. International Edition, Canada, PP. 934-935.
38. Xu, R., Liu, N., Xu, X. and Kong, B. 2011. Antioxidative Effects of Whey Protein on Peroxide-induced Cytotoxicity. J. Dairy Sci., 94(8): 3739-46.
 
Journal of Agricultural Science and Technology
Volume 18, Issue 6
November and December 2016
Pages 1511-1512