Development of an SNP Marker for Sugar Beet Resistance/Susceptible Genotyping to Root-Knot Nematode

Authors
M. Bakooie 1
E. Pourjam 1
S. B. Mahmoudi 2
N. Safaie 1
M. Naderpour 3
1 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
2 Sugar Beet Seed Institute (SBSI), Karaj, Islamic Republic of Iran.
3 Seed and Plant Certification and Registration Institute (SPCRI), Karaj, Islamic Republic of Iran.
Abstract
Linked and/or gene-based molecular markers have been used widely in marker-assisted selection (MAS) to differentiate resistant and susceptible genotypes. Resistance to Meloidogyne spp. in Beta vulgaris L. is mediated by a single dominant gene (R6m-1). Using allele-specific primers (ASPs), an SNP marker harboring a single nucleotide polymorphism (A/G), linked to the resistance gene was developed to differentiate resistant genotypes. The differentiation among the resistant and susceptible genotypes was elucidated in the polymorphic bands of 555, 478 and 124 bp in size, using PCR amplification. The genotyping data using the SNP marker was firmly associated with the bioassay evaluation in the greenhouse for 100 sugar beet genotypes. This data indicated that the present robust marker allowed reliable, sensitive, faster, and cheaper large scale screening of B. vulgaris genotypes for nematode resistance breeding programs.

Keywords

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Journal of Agricultural Science and Technology
Volume 17, Issue 2
March and April 2015
Pages 443-454