Reaction of Iranian Cereal Genotypes to Multiple Strains of Xanthomonas translucens pv. cerealis

Authors
N. Falahi Charkhabi 1
M. Shams-bakhsh 1
H. Rahimian 2
1 Plant Pathology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
2 Department of Plant Protection, Sari University of Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran.
Abstract
Bacterial leaf streak (BLS) caused by Xanthomonas translucens pv. cerealis (Xtc) is an important disease of wheat (Triticum aestivum L.) worldwide. The management methods presently in practice are insufficient to meet current safety and/or efficacy standards. Therefore, use of resistant genotypes is the best approach to manage BLS. The present study was undertaken to identify possible sources of resistance to Xtc in cereal cultivars and germplasm. Twelve strains of Xtc were isolated from symptomatic leaves in several regions in Kerman province. Out of twelve, nine strains produced the expected Xtc-specific 120 bp fragment using PCR and the primer pairs PABr/PBf. Six strains produced water-soaked streaks covered with exudates on wheat cultivars, whereas the three remaining strains incited only chlorotic streaks with no water-soaking on leaves. A highly virulent strain that caused conspicuous water-soaking and necrosis was used for inoculation of 645 winter and spring wheat, barley, and rye accessions to identify possible sources of resistance to BLS. The fourth leaves of test plants were infiltrated with bacterial suspension and scored after seven to ten days. Among all the accessions evaluated, only two rye accessions, namely, 4538 and 4794, were resistant to BLS. These two rye accessions can potentially be used in breeding rye and triticale cultivars for resistance to BLS.

Keywords

1. Adhikari, T. B., Gurung, S., Hansen, J. M. and Bonman, J. M. 2012. Pathogenic and Genetic Diversity of Xanthomonas translucens pv. undulosa in North Dakota. Phytopathol., 102: 390-402.
2. Akhtar, M. A. and Aslam, M. 1985. Bacterial Stripe of Wheat in Pakistan. Rachis, 4: 49.
3. Akhtar, M. and Aslam, M.1986. Xanthomonas campestris pv. undulosa on Wheat. Rachis, 5: 34-37.
4. Alizadeh, A. and Rahimian, H. 1989. Bacterial Leaf Streak of Gramineae in Iran. EPPO Bull., 19:113-117.
5. Alizadeh, A., Barrault, G. and Sarrafi, A. 1995. Identification of Bacterial Leaf Streak of Cereals by Their Phenotypic Characteristics and Host Range in Iran. Eur. J. Plant Pathol., 101: 225-229.
6. Arabi, F. Nickravesh, Z. Babazad, V. Rezaeian, V. and Rahimian, H. 2006. The Occurrence of Bacterial Blight of Beet Caused by Pseudomonas syringae pv. aptata in Iran. Iran. J. Plant Pathol., 42: 471-655.
7. Beiki, F., Alizadeh, A. and Khodakaramian, G. 2004. Genetic Variability for Reaction to Xanthomonas translucens pvs. cerealis and translucens in Wheat and Barley Cultivars. Iran. J. Agric. Sci., 6: 1-6.
8. Boosalis, M. G. 1952. The Epidemiology of Xanthomonas translucens (J. J. and R.) Dowson on Cereals and Grasses. Phytopathol., 42: 387-395.
9. Bragard, C., Singer E., Alizadeh A., Vauterin L., Maraite H. and Swings J. 1997. Xanthomonas translucens from Small Grains: Diversity and Phytopathological Relevance. Bacteriol., 87: 1111-1117.
10. Cunfer, B. M. 1987. Bacterial and Fungal Blights of Foliage and Heads of Wheat. In: “Wheat and Wheat Improvement”, (Ed): Heynes, E. G.. 2nd Edition, ASA, Madison, WI, PP. 528-541.
11. Duveiller, E. 1992. Toward management of bacterial leaf streak of wheat and triticale. PhD. Dissertation, Faculté des Sciences Agronomiques, Université Catholique de Louvain, Louvain-la-Neuve, Belgium, 224 PP.
12. El Attari, H., Sarrafi, A., Garrigues, S., Dechamp-Guillaume, G. and Barrault, G. 1996. Diallel Analysis of Partial Resistance to an Iranian Strain of Bacterial Leaf Streak (Xanthomonas campestris pv. cerealis) in Wheat. Plant Pathol., 45: 1134-1138.
13. Forster, R. L. and Schaad, N. W. 1985. Evaluation of Seed Treatments for Eradication of Xanthomonas campestris pv. translucens from Wheat Seed. Phytopathol., 75: 1385. (Abstract)
14. Forster, R. L. and Schaad, N. W. 1988. Control of Black Chaff of Wheat with Seed Treatment and a Foundation Seed Health Program. Plant Dis., 72: 935-938.
15. Fourest, E., Rehms, L. D., Sands, D. C., Bjarko, M. and Lund, R. E. 1990. Eradication of Xanthomonas translucens from Barley Seed with Dry Heat Treatments. Plant Dis., 74: 816-818.
16. Leben, C. 1981. How Plant-pathogenic Bacteria Survive. Plant Dis., 65: 633-6637.
17. Levy, S. B. 1998. The Challenge of Antibiotic Resistance. Scientific Amer., 278: 46 53.
18. Maraite, H., Bragard, C. and Duveiller, E. 2007. The Status of Resistance to Bacterial Diseases of Wheat. In: Wheat Production in Stressed Environments”, (Eds.): Buck, H. T., Nisi, J. E. and Salomón, N.. Springer, New York, PP. 37-49.
19. Marefat, A., Ophel-Keller, K., Scott, E. S. and Sedgley, M. 2006. The Use of ARMS PCR in Detection and Identification of Xanthomonads Associated with Pistachio Dieback in Australia. Eur. J. Plant Pathol., 116: 57-68.
20. Mehta, Y. R. 1990. Management of Xanthomonas campestris pv. undulosa and hordei through Cereal Seed Testing. Seed Sci. Tech., 18: 467-476.
21. Mehta, Y. R. 1993. Manejo integrado de enfermedades del trigo. Santa Cruz de la Sierra, Bolívia, CIAT/IAPAR, 314 PP.
22. Milus, E. A. and Chalkley, D. B. 1994. Virulence of Xanthomonas campestris pv. translucens on Selected Wheat Genotypes. Plant Dis., 78: 612-615.
23. Milus, E. A. and Mirlohi, A. F. 1994. Use of Disease Reactions to Identify Resistance in Wheat to Bacterial Streak. Plant Dis., 78: 157-161.
24. Milus, E. A., Duveiller, E., Kirkpatrick, T. L. and Chalkey, D. B. 1996. Relationships between Disease Reactions under Controlled Conditions and Severity of Wheat Bacterial Streak in the Field. Plant Dis., 80: 726-730.
25. Mohan, S. K. and Bijman, V. P. 2001. Bacterial Leaf Stripe Caused by Xanthomonas translucens pv. cerealis on Intermediate Wheatgrass in Idaho. Plant Dis., 85: 921-922.
26. Razi Nataj, M. Agha Jani, M. A. and Zand, S. 2010. Bacterila Leaf Blight and Black Chaff in Golestan. Proc. 19th Plant Protection Cong., Tehran, Iran, 423 PP.
27. Sands, D. C., Mizrak, G., Hall, V. N., Kim, H. K., Bockelman, H. E. and Golden, M. J. 1986. Seed Transmitted Bacterial Diseases of Cereals: Epidemiology and Control. Arab. J. Plant Prot., 4: 127-125.
28. Schaad, N. W. and Forster, R. L. 1985. A Semiselective Agar Medium for Isolating Xanthomonas campestris pv. translucens from Wheat Seeds. Phytopathol., 75: 260-263.
29. Smith, E. F., Jones, L. R. and Reddy, C. S. 1919. The Black Chaff of Wheat. Sci. 50: 48.
30. Tillman, B. L., Harrison, S. A., Clark, C. A., Milus, E. A., and Russin, J. S. 1996. Evaluation of Bread Wheat Germplasm for Resistance to Bacterial Streak. Crop Sci. 36:1063–1068.
31. Tubajika, K. M., Tillman, B. L., Russin, J. S., Clark, C. A. and Harrison, S. A. 1998. Relationship between Flag Leaf Symptoms Caused by Xanthomonas translucens pv. translucens and Subsequent Seed Transmission in Wheat. Plant Dis., 82: 1341-1344.
32. Wallin, J. R. 1946. Parasitism of Xanthomonas translucens (J. J. and R.) Dowson on Grasses and Cereals. Iowa State College J. Sci., 20: 171-193.
33. Wiese, M. V. 1987. Compendium of Wheat Diseases. 2nd Edition, APS Press, APS Press, St. Paul, MN. 112pp.
34. Zakeri, Z. and Al-e-Agha, N. 1986. Isolation of Xanthomonas translucens from Barley Native Cultivars in Sistan. Proc. 8th Plant Protection Cong., Isfahan, Iran, 108 PP.
Journal of Agricultural Science and Technology
Volume 17, Issue 1
January and February 2015
Pages 241-248