Mapping QTLs associated with salt tolerance related traits in wheat (Triticum aestivum L.)

Authors
M. Ghaedrahmati 1
M. Mardi 2
M. R. Naghavi 3
E. Majidi Heravan 2
B. Nakhoda 4
A. Azadi 5
M. Kazemi 6
1 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2 Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
3 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran
4 Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
5 Islamic Azad University, Shahr-e-Rey Branch, Department of Plant Breeding, Shahre-Rey, Iran
6 Department of Plant Breeding, Yadegar-e- Imam Khomeini (RAH) Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
Abstract
Salinity stress is a major limitation in wheat production. The lack of economically viable methods for screening salinity tolerance in field is an obstacle to breeders. In this study a population of 254 recombinant inbred lines (RILs), derived from a cross between Roshan × Sabalan was assessed in glasshouse during the seedling phase in order to identify quantitative trait loci (QTLs) for salinity related traits. A genetic linkage map was constructed from 239 markers, namely, 225 Diversity Arrays Technology markers (DArTs) and 14 simple sequence repeats (SSRs) which spanned a total of 1,099.7cM. A total of 31 QTLs for salinity tolerance were identified on 13 chromosomes, contributing more than 50% of the total phenotypic variation. The frequency of Roshan and Sabalan alleles were high at loci on different homeologous groups. Most of the detected QTLs were located on chromosomes 3B, 5B among the 13 chromosomes. Two QTL related fresh weight and height of shoot were detected on 1A and 3A which explained 18% and 12.9% of the total phenotypic variation respectively. Roshan (salt tolerance) alleles were associated with an increase in all traits under both control and stress conditions. SSR markers gwm626 and gwm540 (on chromosomes 6B and 5B, respectively) were tightly linked with different QTLs under control and stress conditions, and explained 21.1% and 8.1% of the total phenotypic variance, respectively. Some of these QTL mapped to genomic regions previously associated with salt tolerance in wheat.

Keywords

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Journal of Agricultural Science and Technology
Volume 16, Issue 6
November and December 2014
Pages 1413-1428