Comparison of P. syringae pv. syringae from Different Hosts Based on Pathogenicity and BOX-PCR in Iran

Authors
G. Najafi Pour 1
S. M. Taghavi 2
1 Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Islamic Republic of Iran
2 Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Islamic Republic of Iran.
Abstract
During 2007-2008, 58 strains of P. syringae pv. syringae (Pss) were isolated from various
Prunus species and other hosts such as sugar beet, pear, quince, oat, millet, wheat, barley,
and rice in Fars, Isfahan, Kohgiloye and Boyer Ahmad, Chahar Mahal-o-Bakhtiari
provinces of Iran. The strains were tested for pathogenicity, the presence of the syrB gene
and BOX PCR (BOX A1R primer). All tested Pss strains were pathogenic on peach
seedlings regardless of their original hosts. A total of 58 isolates of the Pss and Pss IVIA
773-1 amplified a 752-bp fragment with the syrB primers. The results of analysis of the
BOX fingerprints from P. syringae pv. syringae strains showed that the strains isolated
from stone fruits, graminous hosts and pome fruits formed a relatively distinct cluster,
which were separable from the strains isolated from the other hosts. Results of this study
indicate the existence of a relative degree of host specialization within the heterogeneous
pathovar Pss.

Keywords

Journal of Agricultural Science and Technology
Volume 13, Issue 3
May and June 2011
Pages 431-442