Identification of Tobacco Leaf Curl Virus Infecting Lonicera japonica, an Ornamental Plant Common in Japan

Authors
A. Ali 1
M. Ahmed 1
H. Nishigawa 2
T. Natsuaki 2
1 Department of Plant Pathology, Faculty of Crop Protection Sciences, The University of Agriculture, Peshawar, Pakistan.
2 Department of Plant Pathology, Laboratory of Plant Virology, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan.
Abstract
A begomovirus, tentatively named Tobacco leaf curl Japan virus-JpU (TbLCJV-JpU), was isolated from Lonicera japonica (Honeysuckle) Plants Grown in Utsunomiya showing veinal chlorosis symptoms. The TbLCJV-JpU genome with 2,761 nt showed a highest identity with TbLCJV-Jp3 and was also close to TbLCJV and as well to TbLCJV-Jp2. The overall nt identity with TbLCJV-Jp3 amounted to 92.94%, while the identities in encoded amino acid (aa) sequence of Coat Protein (CP) and putative products of AC1 and AV2 ORFs were as high as 98.05, 92.54 and 93.96%, respectively. Low sequence identities were observed in the Intergenic Region (IR) of TbLCJV-JpU as compared with TbLCJV, Ageratum yellow vein Taiwan virus-Kochi isolate and Honeysuckle yellow vein virus-Kagoshima isolates. Recombinations were detected in the 5´end (2650 to 2761) and extreme 3´ portion of the genome (220 to 350). Both regions demonstrated high identities with AYVTV-Kochi and HSYVV-Kagoshima. To the best of our knowledge this is the first report of isolation of TbLCJV from L. japonica.

Keywords

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Journal of Agricultural Science and Technology
Volume 16, Issue 3
May and June 2014
Pages 643-653