Characterization and Identification of Markers Associated with Morphological and Biochemical Traits of Pomegranate Fruit Using SCoT and SSR Markers

Articles in Press

Document Type : Original Research

Authors
Elham Sadeghi Seresht 1
Hamid Reza Karimi 1
Khalil Malekzadeh 2
Seyed Hossein Mirdehghan 1
Ali Akbar Mohammadi Mirik 2
Mohammad Sadegh Jomeyazdiyan 1
1 Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Islamic Republic of Iran.
2 Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Islamic Republic of Iran.
Abstract
This research aimed to identify markers related to morphological and biochemical traits in pomegranate using 15 SCoT and 6 pairs of SSR markers. Based on the results obtained from association analysis using mixed linear modeling (MLM) method, a total of 169 markers related to the studied traits were identified among which 149 were based on SCoT markers and 20 relied on SSR markers. The obtained results showed that SCoT351200 loci associated with vitamin C, titratable acidity, peel soluble solid and aril, PGCT001100 associated to fruit juice pH, titratable acidity, aril soluble solid, peel soluble solid and total aril total phenolic content. In addition, SCoT11800, SCoT21100, SCoT5900, SCoT23900, PGCT001105 and PGCT0020200 loci were highly associated only with fruit diameter, fruit crown length, fruit crown diameter, titratable acidity, seed hardness and fruit juice pH, respectively, which could be considered as informative markers in breeding programs for pomegranate.
Keywords
Association analysis, Informative markers, Pomegranate, Population structure, SSR and SCoT
Subjects
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Available Online from 14 January 2026