Biological Activity of Some Plant Extracts on Varroa Mite (Varroa destructor) and Iranian Honeybee (Apis mellifera meda)

Document Type : Original Research

Authors
Ataollah Rahimi 1
Rukhosh Rashed 2
Banaz Abdulla 3
1 Animal Science Research Department, Kurdistan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Sanandaj, Iran.
2 Department of Plant Protection, College of Agricultural Engineering Science, University of Sulaimani, Sulaimaniyah, Iraq.
3 Department of Biology, College of Education, University of Salahaddin, Erbil, Iraq.
Abstract
Acaricidal activity, Insecticidal activity, Mortality percentage of Varroa mite.



Keywords
Acaricidal activity
Honeybee
Insecticidal activity
Plant extract
Varroa mite
     
1.       Abbott, W. S. 1925. A Method of Computing the Effectiveness of an Insecticide. J. Econ. Entomol., 18(2): 265-267.
2.       Adams, R. P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. 4th Edition, Allured Publishing Corporation, Carol Stream, USA.
3.       Adamczyk, S., Lázaro, R., Pérez-Arquillué, C., Conchello, P. and Herrera, A. 2005. Evaluation of Residues of Essential Oil Components in Honey after Different Anti-Varroa Treatments. J. Agric. Food Chem., 53(26): 10085–10090.
4.       Bahreini, R., González-Cabrera, J., Hernández-Rodríguez, C. S., Moreno-Martí, S., Muirhead, S., Labuschagne, R. B. and Rueppe, L. R. 2025. Arising Amitraz and Pyrethroids Resistance Mutations in the Ectoparasitic Varroa destructor Mite in Canada. Sci. Rep., 15: 1587.
5.       Calderone, W., William, T. and Spivak, S. 1997. Plant Extracts Used for Control of the Parasitic Mites Varroa jacobsoni (Acari: Varroidae) and Acarapis woodi (Acari: Tarsonemidae) in Colonies of Apis mellifera (Hymenoptera: Apidae). J. Econ. Entomol., 90(5): 10801086.
6.       Damiani, N., Liesel, B., Matías, D., Marcangeli, A. and Eguaras, J. 2022. Repellent and Acaricidal Effects of Botanical Extracts on Varroa destructor. Parasitol. Res., 108(1): 79–86.
7.       Delaplane, K.S., Van der Steen, J. and Guzmán-Novoa, E. 2013. Standard Methods for Estimating Strength Parameters of Apis mellifera colonies. J. Apic. Sci., 52(1): 1-12.
8.       Dietemann, V., Nazz, F., Martin, S. J., Anderson, D. L., Locke, B., Delaplane, K. S., Wauquiez, Q., Tannahill, C., Frey, E., Ziegelmann, B., Rosenkranz, P. and Ellis, J. D. 2013. Standard Methods for Varroa Research. J. Apic. Res., 52(1): 1-54.
9.       Farhadi, F., Iranshahi, M., Taghizadeh, S. F. and Asilia, J. 2020. Volatile Sulfur Compounds: The Possible Metabolite Pattern to Identify the Sources and Types of Asafoetida by Headspace GC/MS Analysis. Ind. Crops Prod., 155(3): 112827.
10.    Farid, P., Ghasemi, Y., Gholami, A., Mehregan, I. and Mohagheghzadeh, A.
2008. Antimicrobial Essential Oil from Smyrniopsis aucheri. Chem. Nat. Compd., 44(1): 116-118.
11.    Giusti, M., Sabelli, C., Di, Donato, A., Lamberti, D., Paturzo, C.E., Polignano, V., Lazzari, R. and Felicioli, A. 2017. Efficacy and Safety of Var Terminator, a New Formic Acid Medicine against the Varroa Mite. J. Apic. Res., 56(13): 162-167.
12.    Islam, N., Amjad, M., Haq, E. and Naz, F. 2020. Comparative Field Efficiency of the Extracts of Plant Materials for Controlling Varroa destructor about Brood Development in Honey Bee (Apis mellifera) Colonies. Int. J. Biosci., 16(1): 126-138.
13.    Islam, N., Amjad, M., Ehsan-ul-Haq, M., Stephen, E. and Naz, N. 2016. Management of Varroa destructor by Essential Oils and Formic Acid in Apis mellifera Linn. Colonies. J. Entomol. Zool. Stud., 4(8): 97-104.
14.    Radakovic, M., Stevanovic, J., Djelic, N., Lakic, N., Knezevic-Vukcevic, J., Vukovic-Gacic, B. and Stanimirovic, Z. 2013. Evaluation of the DNA Damaging Effects of Amitraz on Human Lymphocytes in the Comet Assay. J. Biosci., 38(1): 53-62.
15.    Rahimi, A. and Parichehreh, S. 2024. Evaluation and Introduction of a New Plant-Based Formulation to Control Varroa Mite (Varroa destructor) in Honey Bee Colonies (Apis mellifera). J. Entomol. Soc. Iran, 44(4): 417-428.
16.    Rahimi, A., Kahrizi, D., Mirmoayedi, A., Zarei, L. and Jamali, S. 2023. Genetic Characterizations of the Iranian Honey Bee (Apis mellifera meda Skorikov 1929) Populations Using the Microsatellite DNA Markers. Biochem. Genet., 61(6): 2293-2317.
17.    Rahimi, A., Mirmoayedi, A., Kahriz, D., Zarei, L. and Jamali, J. 2022. Molecular Genetic Diversity and Population Structure of Iranian Honey Bee (Apis mellifera meda) Populations: Implications for Breeding and Conservation. J. Plant Dis. Prot., 129(2): 1331–1342.
18.    Ramsey, S. D., Ochoa, R., Bauchan, G., Gulbronson, C., Mowery, J. D., Mowery, A., Lim, D., Joklik, J., Cicero, J. M., Ellis, J. D., Hawthorne, D. and van Engelsdorp, D. 2019. Varroa destructor Feeds Primarily on Honey Bee Fat Body Tissue and Not Hemolymph. Proc. Natl. Acad. Sci. U. S. A., 16(5): 1792-1801.
19.    Ramzanpour, A. and Yakhchali, M. 2020. Study on the Effect of Ferula pseudalliacea (Family: Apiacea) Extract on Varroa destructor (Acari: Varroidae) Infestation in Honeybee (Hymenoptera: Apidae, Apis melifera) by Injection Method. Iran. J. Vet. Res., 15(4): 85-92.
20.    Ramzia, H., Ismailib, M. R., Aberchaneb, M. and Zaanouna, S. 2017. Chemical Characterization and Acaricidal Activity of Thymus satureioides C. & B. and Origanum elongatum E. & M. (Lamiaceae) Essential Oils against Varroa destructor Anderson & Trueman (Acari: Varroidae). Ind. Crops Prod., 108(3): 201-207.
21.    Sammataro, D., Degrandi-Hofman, G., Needham, G. and Wardell, G. 1998. Some Volatile Plant Oils as Potential Control Agents for Varroa Mites (Acari: Varroidae) in Honey Bee Colonies (Hymenoptera: Apidae). Am. Bee J., 138(9): 681–685.
22.    Sanda, K., Koba, K., Akpagana, K. and Tchepan, T. 2001. Teneur et Compostion Chimique de L´huile Essentielle de Ocimum basilicum L. et Ocimum gratissicum L. a Diferentes Periodes de Recolte Apres Semis. Rivista Italiana EPPOS, 31(2): 3–7.
23.    Shaddel Tilly, A., Mahri Sis, N., Aghajanzadeh Golshani, A., Asadi Disney, A. and Ahmadzadeh, A. 2008. Investigating the Effect of Using Tobacco, Pecan and Mountain Thyme Plants in Controlling Honey Bee Varroa Mite. J. Agric. Sci., 4(2): 67-72.
24.    Tavassoli, M., Ghanbarpoor, L. and Shamsi, L. 2021. Study of Pesticide Effects of Matricaria chamomilla Extract on Argas persicus Ticks. J. Vet. Res., 73(2): 135-139.
25.    Zemene, M., Bogale, B., Derso, S., Belete, S., Melaku, S. and Hailu, H. 2015. A Review on Varroa Mites of Honey Bees. Acad. J. Entomol., 8(3): 150-159.
 
Journal of Agricultural Science and Technology
Volume 28, Issue 4
July and August 2026
Pages 907-918