Polymorphisms of the Self-Incompatibility Locus of Sweet Cherry (Prunus avium L.) Species from the VNIISPK Bio-Resource Collection

Document Type : Original Article

Authors
Elena Bezlepkina
Aleksandra Guliaeva
Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530 Orel, Russia.
10.48311/jast.2026.24109
Abstract
Self-incompatibility is a common evolutionary mechanism of flowering plants. Sweet cherry (Prunus avium L.) has a gametophytic type of self-incompatibility. Most of sweet cherry cultivars are self-incompatible, although self-compatible genotypes also occur. This study presents data of S genotype investigation of 39 sweet cherry cultivars from VNIISPK bio-resource collection. To identify S genotypes, PCR with consensus (PaConsI and PaConsII) and allele-specific (S1, S2, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S13, S14 and S16) primers was done. The S genotype was fully identified for 33 cultivars. Then, 7 S alleles (S1, S3, S4, S5, S6, S9, and S13) were detected in the genomes of the investigated sweet cherries. According to the results of allele-specific amplification, ‘Venera’ (S1x/S4), ‘Zolotuhinskaya’ (S4x/S6) and ‘Viskochka’ (S3/S4x) were suspected to have unique or mutant alleles of the gene. The cultivars with fully determined S-genotypes were distributed into nine incompatibility groups (III, VI, VII, IX, XV, XVII, XIX, XXI, and XLV).


Keywords
Consensus primers
Allele-specific primers
Subjects
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Journal of Agricultural Science and Technology
Volume 28, Issue 5
September and October 2026
Pages 1185-1194