Polymorphisms of the self-incompatibility locus of sweet cherry (Prunus avium L.) species from the VNIISPK bioresource collection

Articles in Press
Authors
A. Guliaeva
E. Bezlepkina
Russian Research Institute of Fruit Crop Breeding (VNIISPK)
Abstract
Self-incompatibility is a common evolutionary mechanism of flowering plants. Sweet cherry (Prunus avium L.) has a gametophytic type of self-incompatibility. Most of sweet cherry cultivars are self-incompatible. Although self-compatible genotypes also occur. This study presents data of S genotype investigation of 39 sweet cherry cultivars from VNIISPK bioresource collection. To identify S genotypes, PCR with consensus (PaConsI and PaConsII) and allele-specific (S1, S2, S3, S4, S5, S6, S7, S9, S10, S12, S13, S14 and S16) primers was done. The S genotype was fully identified for 33 cultivars. 7 S alleles (S1, S3, S4, S5, S6, S9, and S13) were detected in the genomes of investigated sweet cherries. According to the results of allele-specific amplification, ‘Venera’ (S1x/S4), ‘Zolotuhinskaya’ (S4x/S6) and ‘Viskochka’ (S3/S4x) are suspected to have unique or mutant alleles of the gene. The cultivars with fully determined S-genotypes were distributed into nine incompatibility groups (III, VI, VII, IX, XV, XVII, XIX, XXI, and XLV).

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Journal of Agricultural Science and Technology

Articles in Press, Accepted Manuscript
Available Online from 16 September 2025