Enhancing the Shelf Life and Sensory Properties of Rainbow Trout Fillets through Sodium Alginate Coating Containing Eryngium campestre Extract at 4°C

Document Type : Original Research

Authors
Mohammad Abedi
Hossein Tajik
Tooraj Mehdizadeh
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Islamic Republic of Iran.
Abstract
Fresh fish is a highly perishable food item and spoils easily. In this research, after investigating the antioxidant properties of the Eryngium Campestre extract (Ece), its effect along with the sodium alginate coating was evaluated on the shelf-life of rainbow trout under refrigerated conditions (4°C) for 12 days. To assess the antioxidant properties of the extract, tests such as DPPH, total phenolics, reducing power, and ABTS were performed. Subsequently, samples treated with an Ece containing alginate coating applied via spray method were analyzed for chemical parameters (TBA, TVN, and pH), microbiological parameters (total psychrotrophic and mesophilic bacteria), and sensory evaluations at four day intervals up to 12 days. The results indicated that Ece possessed significant antioxidant properties. Furthermore, treatments that included the extract combined with the sodium alginate coating significantly reduced pH, TVN, and TBA levels compared to the control sample (P< 0.05). Microbial tests indicated that all treated samples inhibited bacterial growth, with a reduction of approximately 3 log CFU g-1 compared to the control group. In the sensory evaluation, treatments containing Ece and sodium alginate yielded more favorable results than those of the control group. Accordingly, coating the samples with sodium alginate and Ece improved the microbial, chemical, and sensory properties and shelf life of rainbow trout in refrigerator conditions by about four days.
 

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1.       Al-Askar, A. A., Bashir, S., Mohamed, A. E., Sharaf, O. A., Nabil, R., Su, Y., Abdelkhalek, A. and Behiry, S. I. 2023. Antimicrobial Efficacy and HPLC Analysis of Polyphenolic Compounds in a Whole-Plant Extract of Eryngium campestre. Separations, 10(6): 362 .
2.       Alizadeh, A., Mehdizadeh, T. and Tajik, H. 2021. Comparative Study of Antioxidant and Antimicrobial Properties of Mentha Aquatica L. Ethanolic Extract and Essential Oil‌. Stud. Med. Sci., 31(11): 863-873. (in Persian with English Abstract)
3.       Alizadeh Amoli, Z., Mehdizadeh, T., Tajik, H. and Azizkhani, M. 2019. Shelf Life Extension of Refrigerated, Vacuum-Packed Rainbow Trout Dipped in an Alginate Coating Containing an Ethanolic Extract and/or the Essential Oil of Mentha Aquatica. Chem. Pap., 73(10): 2541–2550.
4.       Azizkhani, M. and Sodanlo, A. 2021. Antioxidant Activity of Eryngium campestre L., Froriepia subpinnata, and Mentha spicata L. Polyphenolic Extracts Nanocapsulated in Chitosan and Maltodextrin. J. Food Process. Preserv., 45(2): e15120.
5.        Bazargani-Gilani, B. 2018. Activating Sodium Alginate-Based Edible Coating Using a Dietary Supplement for Increasing the Shelf Life of Rainbow Trout Fillet during Refrigerated Storage (4±1°C). J. Food Saf., 38(1): e12395.
6.       Bazargani-Gilani, B. and Pajohi-Alamoti, M. 2020. The Effects of Incorporated Resveratrol in Edible Coating Based on Sodium Alginate on the Refrigerated Trout (Oncorhynchus mykiss) Fillets’ Sensorial and Physicochemical Features. Food Sci. Biotechnol., 29(2): 207–216.
7.       Charoenphun, N., Rajasekaran, B., Palanisamy, S. and Venkatachalam, K. 2023. Impact of Longkong Pericarp Extract on the Physicochemical Properties of Alginate-Based Edible Nanoparticle Coatings and Quality Maintenance of Shrimp (Penaeus monodon) during Refrigerated Storage. Foods, 12(5): 1103.
8.       Ebrahimi, A. H. and Larypoor, M. 2022. The Effect of Aqueous and Alcoholic Extracts of Menthe piperata Carrier of Nanocytosan on Physicochemical and Sensory Properties of Rainbow Trout Fillet. Aqua. Sci., 10(1): 154–164.
9.       Fadıloğlu, E. E. and Emir Çoban, Ö. 2018. Effects of Chitosan Edible Coatings Enriched with Sumac on the Quality and the Shelf Life of Rainbow Trout (Oncorhynchus mykiss), Walbaum, 1792) Fillets. J. Food Saf., 38(6): e12545.
10.     Fernandes, E. S. 2013. Identication of Active Compounds in Extracts of Eryngium Species from the Iberia. Master's Thesis, Universidade de Coimbra, Portugal.
11.    Foromandi, M. and Khani, M. 2023. The Effect of Chitosan Coatings Enriched with Garlic Extract and Coriander Essential Oil on the Shelf Life of Rainbow Trout Fillet Stored at 4 °C. Int. J. Food Sci. Technol., 58(7): 3618–3627.
12.     Gharedaghi, J., Aliakbarlu, J. and Tajik, H. 2020. Antioxidant Potential of Apple Pomace Extract and Its Efficacy in Alginate Coating on Chemical Stability of Rainbow Trout Fillet. J. Food Meas. Charact., 14(1): 135–141.
13.    Ghasemi, S., Jaldani, S., Sanaei, F., Ghiafehshirzadi, A., Alidoost, A., Hashemi, M., Hossaeini Marashi, S. M., Khodaiyan, F. and Noori, S. M. A. 2023. Application of Alginate Polymer Films and Coatings Incorporated with Essential Oils in Foods: A Review of Recent Literature with Emphasis on Nanotechnology. Int. J. Food Eng., 19(3–4): 73–78.
14.    Kartal, M., Mitaine-Offer, A. C., Paululat, T., Abu-Asaker, M., Wagner, H., Mirjolet, J. F., Guilbaud, N. and Lacaille-Dubois, M. A. 2006. Triterpene Saponins from Eryngium campestre. J. Nat. Prod., 69(7): 1105–1108.
15.    Kikowska, M. and Thiem, B. 2021. In Vitro Systems of Selected Eryngium Species (E. planum, E. campestre, E. maritimum, and E. alpinum) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil). Plant Cell Tissue Differ. Second. Metabolites, 869–901.
16.    Kremer, D., Končić, M. Z., Kosalec, I., Košir, I. J., Potočnik, T., Čerenak, A., Srečec, S., Dunkić, V. and Vuko, E. 2021. Phytochemical Traits and Biological Activity of Eryngium amethystinum and E. alpinum (Apiaceae). Horticulturae, 7(10): 364.
17.     Mazandrani, H. A., Javadian, S. R. and Bahram, S. 2016. The Effect of Encapsulated Fennel Extracts on the Quality of Silver Carp Fillets during Refrigerated Storage. Food Sci. Nutr., 4(2): 298–304.
18.    Merghache, D., Boucherit-Otmani, Z., Merghache, S., Chikhi, I., Selles, C. and Boucherit, K. 2014. Chemical Composition, Antibacterial, Antifungal and Antioxidant Activities of Algerian Eryngium tricuspidatum L. Essential Oil. Nat. Prod. Res., 28(11): 795–807.
19.    Mishra, D. and Patnaik, S. 2020. GC-MS Analysed Phyto-Chemicals and Antibacterial Activity of Withania somnifera (L.) Dunal Extract in the Context of Treatment to Liver Cirrhosis. Biomed. Pharmacol. J., 13(1): 71–78.
20.    Muñoz-Tebar, N., Pérez-Álvarez, J. A., Fernández-López, J. and Viuda-Martos, M. 2023. Chitosan Edible Films and Coatings with Added Bioactive Compounds: Antibacterial and Antioxidant Properties and Their Application to Food Products: A Review. Polymers, 15(2): 396.
21.    Ojagh, S. M., Rezaei, M., Razavi, S. H. and Hosseini, S. M. H. 2010. Effect of Chitosan Coatings Enriched with Cinnamon Oil on the Quality of Refrigerated Rainbow Trout. Food Chem., 120(1): 193–198.
22.     Öz, M. 2018. Effects of Garlic (Allium sativum) Supplemented Fish Diet on Sensory, Chemical and Microbiological Properties of Rainbow Trout during Storage at −18°C. LWT, 92: 155–160.
23.     Öz, M., Dikel, S., Durmuş, M. and Özoğul, Y. 2017. Effects of Black Cumin Oil (Nigella sativa) on Sensory, Chemical and Microbiological Properties of Rainbow Trout during 23 Days of Storage at 2±1°C. J. Aqua. Food Prod. Technol., 26(6): 665–674.
24.    Ozogul, Y., Yuvka, İ., Ucar, Y., Durmus, M., Kösker, A. R., Öz, M. and Ozogul, F. 2017. Evaluation of Effects of Nanoemulsion Based on Herb Essential Oils (Rosemary, Laurel, Thyme and Sage) on Sensory, Chemical and Microbiological Quality of Rainbow Trout Fillets during Ice Storage. LWT, 75: 677–684.
25.    Popelka, P., Marcinčák, S., Maskal’ová, I., Guothová, L. and Čertík, M. 2014. Comparison of the Chemical Composition and Nutritional Values of Fresh and Frozen Rainbow Trout. Slov. Vet. Res., 51(2): 73-80.
26.    Raeisi, M., Hashemi, M., Aminzare, M., Ghorbani Bidkorpeh, F., Ebrahimi, M., Jannat, B., Tepe, B. and Noori, S. M. A. 2020. Effects of Sodium Alginate and Chitosan Coating Combined with Three Different Essential Oils on Microbial and Chemical Attributes of Rainbow Trout Fillets. J. Aqua. Food Prod. Technol., 29(3): 253-263.
27.    Rathod, N. B., Ranveer, R. C., Benjakul, S., Kim, S. K., Pagarkar, A. U., Patange, S. and Ozogul, F. 2021. Recent Developments of Natural Antimicrobials and Antioxidants on Fish and Fishery Food Products. Compr. Rev. Food Sci. Food Saf., 20(4): 4182–4210.
28.     Sallam, K. I. 2007. Antimicrobial and Antioxidant Effects of Sodium Acetate, Sodium Lactate, and Sodium Citrate in Refrigerated Sliced Salmon. Food Control, 18(5): 566-575.‏
29.     Shakour, N., Khoshkhoo, Z., Akhondzadeh Basti, A., Khanjari, A. and Mahasti Shotorbani, P. 2021. Investigating the properties of PLA-nanochitosan composite films containing Ziziphora Clinopodioides essential oil and their impacts on oxidative spoilage of Oncorhynchus mykiss fillets. Food Science and Nutrition. 9(3): 1299–1311.
30.     Torabi Delshad, S., Sabetian, M., Moini, S., Rajabi Islami, H. and Motalebi, A. 2012. Identification of Fatty Acid Content, Amino Acid Profile and Proximate Composition in Rainbow Trout. J. Am. Sci., 8(4).
31.     Urbonavičiūtė, G., Dyglė, G., Černauskas, D., Šipailienė, A., Venskutonis, P. R. and Leskauskaitė, D. 2023. Alginate/Pectin Film Containing Extracts Isolated from Cranberry Pomace and Grape Seeds for the Preservation of Herring. Foods, 12(8):1678.
32.    Venkatachalam, K., Charoenphun, N., Lekjing, S. and Noonim, P. 2023. Investigation of Melatonin Incorporated CMC-Gelatin Based Edible Coating on the Alleviation of Chilling Injury Induced Pericarp Browning in Longkong. Foods, 13(1): 72.
33.    Zarandi, M., Hasani, M., Shotorbani, P. M., Basti, A. A. and Hamedi, H. 2022. Assessing Edible Composite Coating of Sodium Alginate–Galbanum Gum Impregnated with Nettle Extract on Improving the Shelf Life of Rainbow Trout Fillet. J. Food Meas. Charact., 16(4): 2556–2570.
Journal of Agricultural Science and Technology
Volume 28, Issue 2
March and April 2026
Pages 335-350