Flow Cytometric Analysis of Programmed Cell Death in Rose (Rosa hybrida cv. Dolce vita+) as Influenced by Physicochemical Treatments

Document Type : Original Research

Authors
G. Karimzadeh 1
S. Farhadi 1
A. Baghizadeh 2
V. Sayadi 1
1 Department of Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, P. O. Box: 14115-336, Islamic Republic of Iran.
2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Islamic Republic of Iran.
10.22034/JAST.27.4.861
Abstract
This study aimed to increase the vase life of cut rose flowers by improving the regulation of Programmed Cell Death (PCD). Experiments were carried out on cut rose (Rosa hybrida cv. Dolce vita+) flowers under either physical treatment of Static Magnetic Field (SMF: 15 and 25 mT) for 3 hours, or chemical treatments of silver nano particle (Nano-Ag: 5 and 10 ppm), 6-Benzyladenine (BA: 25 and 50 mg L-1), 1% sucrose, and combinations of 5 and 10 ppm nano-Ag with 3 and 6% sucrose. Results showed that a 15 mT-SMF significantly increased vase life up to 25 days, compared to the controls and to all chemical treatments. Among the chemicals, 5 ppm Nano-Ag and 1% (w/v) sucrose increased vase life to 23 and 18 days, respectively. The smallest decline in fresh weight was observed in the 15 mT-SMF physical treatment. Markedly, the 15 mT-SMF treatment led to the least reduction in Chlorophyll (Chl) content. On the 17th day of the applied different treatments, both Water Uptake (WU) and Relative Fresh Weight (RFW) showed an inverse significant relationship with PCD in cut rose flowers, verifying the delayed PCD, which is favored in the market. As a whole, the most effective induced treatments (15 mT-SMF, 5 ppm nano-Ag, and 1% sucrose) are suggested to be promising for enhancing postharvest quality and prolonged vase life of cut rose flowers.

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Journal of Agricultural Science and Technology
Volume 27, Issue 4
July and August 2025
Pages 861-877