Antifungal potential of Lactiplantibacillus plantarum AM2 against the banana pathogen Fusarium oxysporum f. sp. cubense Tropical Race 4

Document Type : Original Research

Authors
W. A. N. Wan Tajudin Shah 1
N. B. Abd Murad 1
J. X. Ong 1
S. H. Ang 1
N. Laili 2
N. B. Saidi 3
1 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University of Putra Malaysia, Serdang, Selangor, Malaysia.
2 Research Center for Applied Microbiology, Research Organization for Life Sciences, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Bogor, West Java 16911, Indonesia.
3 Laboratory of Sustainable Agronomy and Crop Protection, Institute of Plantation Studies, University of Putra Malaysia, Serdang, Selangor, Malaysia.
10.22034/JAST.27.4.763
Abstract
Fusarium wilt of bananas is a serious disease affecting banana plantations worldwide. In an effort to sustainably manage the disease, biological control is considered a promising alternative to agrochemicals that can cause detrimental effects on humans and the ecosystem. In this study, we investigated the biological control potential of the present collection of beneficial bacteria, including Lactiplantibacillus plantarum AM2, Streptomyces morookaensis NRRL B-12429, Bacillus velezensis B4158, B. atrophaeus B363B, and B. amyloliquefaciens B942, through a dual culture assay and a greenhouse experiment. The studied causal agent of Fusarium wilt in banana was Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4). The inhibition range in vitro was between 31.0% to 42.1%, and the highest growth inhibition of Foc TR4 was observed for L. plantarum AM2. Infected banana plantlets that received the treatment with L. plantarum AM2 also showed a significant reduction in disease severity index, as low as 24% compared to the treatment with other beneficial bacteria. This study showed that L. plantarum AM2 has a good antagonistic effect on Foc TR4 mycelial growth and the most potential to control Fusarium wilt disease in bananas.

Keywords

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Journal of Agricultural Science and Technology
Volume 27, Issue 4
July and August 2025
Pages 763-773