How Different Temperatures and Feeding Rates Impact Physiological and Histological Responses of Juvenile Asian Seabass (Lates calcarifer)

Document Type : Original Research

Authors
1 Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr, Islamic Republic of Iran.
2 Persian Gulf Research Institute, Persian Gulf University, Bushehr, Islamic Republic of Iran.
3 Jihoceská univerzita v Ceských Budejovicích, Fakulta rybárství a ochrany vod, Jihoceské výzkumné centrum akvakultury a biodiverzity hydrocenóz, Ústav akvakultury a ochrany vod, Na Sádkách 1780, 370 05 Ceské Budejovice.
Abstract
This study evaluated the interactive impacts of water temperature and feeding rate on digestive enzymes, intestine histology, growth and stress-related genes, and cultivable intestinal microbiota of Asian seabass (Lates calcarifer). For this purpose, 180 fish (85.0±3.0 g) were reared at three different temperatures (20, 27, and 33°C) and two feeding rates (apparent satiation and 2.5% of biomass) with three replications for 6 weeks. The results revealed no significant differences among different treatments regarding the activity of digestive enzymes (P˃ 0.05) of fish reared under different temperatures and feeding rates. The length, width, and thickness of intestinal villi were unaffected by different temperatures and feeding rates (P˃ 0.05). In addition, no variations were found in the total aerobic bacterial count of fish gut from different experimental groups (P˃ 0.05). At the molecular level, IGF-I and HSP70 coding genes were found to be highly expressed in experimental treatments (P< 0.05). To conclude, the present study showed that temperatures between 27 to 33°C were more optimal for Asian seabass, and the different temperatures and feeding rates did not affect the digestive enzymes, intestine histology, and gut microbiota of juvenile Asian seabass after 6 weeks.

Keywords


Ananthan, J., Goldberg, A. L. and Voellmy, R. (1986). Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes. Science, 232(4749): 522–524.
Anson, M. L. (1938). The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. The Journal of General Physiology, 22(1): 79-89.
Baloi, M. F., Sterzelecki, F. C., Sugai, J. K., Passini, G., Carvalho, C. V. A. and Cerqueira, V. R. (2017). Growth performance, body composition and metabolic response to feeding rates in juvenile Brazilian sardine Sardinella brasiliensis. Aquaculture Nutrition, 23(6): 1458–1466.
Beckman, B. R., Larsen, D. A., Moriyama, S., Lee-Pawlak, B. and Dickhoff, W. W. (1998). Insulin-like growth factor-I and environmental modulation of growth during smoltification of spring chinook salmon (Oncorhynchus tshawytscha). General and Comparative Endocrinology, 109(3): 325–335.
Bowyer, J. N., Qin, J. G., Adams, L. R., Thomson, M. J. S. and Stone, D. A. J. (2012). The response of digestive enzyme activities and gut histology in yellowtail kingfish (Seriola lalandi) to dietary fish oil substitution at different temperatures. Aquaculture, 368: 19–28.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-54.
Chauvigné, F., Gabillard, J.-C., Weil, C. and Rescan, P. Y. (2003). Effect of refeeding on IGFI, IGFII, IGF receptors, FGF2, FGF6, and myostatin mRNA expression in rainbow trout myotomal muscle. General and Comparative Endocrinology, 132(2): 209–215.
Deane, E. E. and Woo, N. Y. S. (2005). Cloning and characterization of the hsp70 multigene family from silver sea bream: modulated gene expression between warm and cold temperature acclimation. Biochemical and Biophysical Research Communications, 330(3): 776–783.
Fang, J., Tian, X. and Dong, S. (2010). The influence of water temperature and ration on the growth, body composition and energy budget of tongue sole (Cynoglossus semilaevis). Aquaculture, 299(1–4): 106–114.
Fauconneau, B. (1985). Protein synthesis and protein deposition in fish. Nutrition and Feeding in Fish, 17–45.
Fry, F. E. J. (1971). The effect of environmental factors on the physiology of fish. Fish Physiology, 1–98.
Furné, M., García-Gallego, M., Hidalgo, M. C., Morales, A. E., Domezain, A., Domezain, J. and Sanz, A. (2008). Effect of starvation and refeeding on digestive enzyme activities in sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 149(4): 420–425.
Groot, C., Margolis, L., Clarke, W. C. and Saunders, R. L. (1996). Physiological ecology of pacific salmon. Reviews in Fish Biology and Fisheries, 6(4): 463-464.
Hagi, T., Tanaka, D., Iwamura, Y. and Hoshino, T. (2004). Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish. Aquaculture, 234(1–4): 335–346.
Harpaz, S., Hakim, Y., Slosman, T. and Eroldogan, O. T. (2005). Effects of adding salt to the diet of Asian sea bass Lates calcarifer reared in fresh or salt water recirculating tanks, on growth and brush border enzyme activity. Aquaculture, 248(1–4): 315–324.
Horsley, R. W. (1977). A review of the bacterial flora of teleosts and elasmobranchs, including methods for its analysis. Journal of Fish Biology, 10(6): 529–553.
Huyben, D., Sun, L., Moccia, R., Kiessling, A., Dicksved, J. and Lundh, T. (2018). Dietary live yeast and increased water temperature influence the gut microbiota of rainbow trout. Journal of Applied Microbiology, 124(6): 1377–1392.
Jerry, D. R. (2013) Biology and culture of Asian seabass Lates calcarifer. CRC Press.
Jobling, M. (1981). The influences of feeding on the metabolic rate of fishes: a short review. Journal of Fish Biology, 18(4): 385–400.
Kestemont, P. and Baras, E. (2001). Environmental factors and feed intake: mechanisms and interactions. Food Intake in Fish, 131–156.
Kim, T. G., Yun, J., Hong, S.-H. and Cho, K.S. (2014). Effects of water temperature and backwashing on bacterial population and community in a biological activated carbon process at a water treatment plant. Applied Microbiology and Biotechnology, 98(3): 1417–1427.
Ley, R. E., Hamady, M., Lozupone, C., Turnbaugh, P. J., Ramey, R. R., Bircher, J. S., Schlegel, M. L., Tucker, T. A., Schrenzel, M. D. and Knight, R. (2008). Evolution of mammals and their gut microbes. Science, 320(5883):1647–1651.
Liston, J. (1957). The occurrence and distribution of bacterial types on flatfish. Microbiology, 16(1): 205–216.
Livak, K. J. and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25(4): 402-408.
Ming, C., Rui, W., Liping, L., Huang, T., Weiyi, H., Jian, L., Chao, L., Aiying, L., Honglin, L. and Wanwen, L. (2013). Sequence and evolution differences of Oreochromis niloticus CXC contribute to the diversification of cellular immune responses in tilapias with treatment of Streptococcus iniae. Journal of Animal and Veterinary Advances, 12(3): 303–311.
Pedersen, T. and Jobling, M. (1989). Growth rates of large, sexually mature cod Gadus morhua, in relation to condition and temperature during an annual cycle. Aquaculture, 81(2): 161–168.
Podrabsky, J. E. and Somero, G. N. (2004). Changes in gene expression associated with acclimation to constant temperatures and fluctuating daily temperatures in an annual killifish Austrofundulus limnaeus. Journal of Experimental Biology, 207(13): 2237–2254.
Rawling, M. D., Merrifield, D. L. and Davies, S. J. (2009). Preliminary assessment of dietary supplementation of Sangrovit®on red tilapia (Oreochromis niloticus) growth performance and health. Aquaculture, 294(1–2): 118–122.
Roberts, R. J. (2012). Fish pathology. John Wiley and Sons.
Soriano, E. L., Ramírez, D. T., Araujo, D. R., Gómez-Gil, B., Castro, L. I. and Sánchez, C. G. (2018). Effect of temperature and dietary lipid proportion on gut microbiota in yellowtail kingfish Seriola lalandi juveniles. Aquaculture, 497: 269–277.
Sugita, H., Iwata, J., Miyajima, C., Kubo, T., Noguchi, T., Hashimoto, K. and Deguchi, Y. (1989). Changes in microflora of a puffer fish Fugu niphobles, with different water temperatures. Marine Biology, 101(3): 299–304.
Temming, A. and Herrmann, J. P. (2001). Gastric evacuation of horse mackerel. II. The effects of different prey types on the evacuation model. Journal of Fish Biology, 58(5): 1246–1256.
Volkoff, H. and Rønnestad, I. (2020). Effects of temperature on feeding and digestive processes in fish. Temperature, 7(4): 307–320.
Wang, N., Xu, X. and Kestemont, P. (2009). Effect of temperature and feeding frequency on growth performances, feed efficiency and body composition of pikeperch juveniles (Sander lucioperca). Aquaculture, 289(1–2): 70–73.
Wong, S. and Rawls, J. F. (2012). Intestinal microbiota composition in fishes is influenced by host ecology and environment. Molecular Ecology, 21 (13): 3100-3102.