Morpho-Physiological Responses of Cucumis sativus L. Seedlings to Various Culture Media

Document Type : Original Research

Authors
1 Department of Agricultural Engineering, University of Hormozgan, Bandar Abbas, Islamic Republic of Iran.
2 Department of Horticultural Science, Faculty of Agriculture, University of Jiroft, Jiroft, Islamic Republic of Iran.
3 Department of Biology, Faculty of Science, University of Jiroft, Jiroft, Islamic Republic of Iran.
Abstract
Various culture media contain a variety of materials that affect plant growth and development. Finding the best media culture among the various materials is thus critical to plant productivity. This study was conducted based on a completely randomized design using eight treatments and three replications. The treatments included the ratios 30:10:60 of perlite-vermicompost-coco peat, peat moss-vermicompost-palm peat, coco peat-vermicompost-palm peat, perlite-vermicompost-palm peat, and the ratios 30:70 of peat moss-palm peat, coco peat-palm peat, perlite-palm peat, and vermicompost-palm peat. Cucumber seeds were planted in pots containing these culture media under greenhouse conditions. After the four-leaf stage, the morpho-physiological responses of the seedlings were evaluated. According to the findings, when compared to perlite-vermicompost-coco peat medium, peat moss-vermicompost-palm peat increased shoot fresh weight by 5.5-fold, root fresh weight by 4.5-fold, root dry weight by 5.8-fold, shoot dry weight by 7-fold, stem diameter by 1.7-fold, shoot length by 2.3-fold, root length by 1.3-fold, and leaf area by 3.8-fold. However, compared to seedlings grown in peat moss-vermicompost-palm peat medium, the amount of auxin and reactive oxygen species decreased while total soluble sugars and peroxidase increased in perlite-vermicompost-coco peat medium. Our findings indicate that the composition of peat moss-vermicompost-palm peat can be used as a beneficial medium to improve quality of cucumber seedling under greenhouse conditions.

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Ainiwaer, M., Ding, J., Kasim, N., Wang, J. Z. and Wang, J. J. 2020. Regional scale soil moisture content estimation based on multi-source remote sensing parameters. Int. J. Remote Sens. 41: 3346–3367. https://doi.org/10.1080/01431161.2019.1701723.
Albalasmeh, A. A., Berhe, A. A. and Ghezzehei, T. A. 2013. A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry. Carbohydr. Polym. 97(2): 253-261. https://doi.org/10.1016/j.carbpol.2013.04.072.
Ali, M., Griffiths, A. J., Williams, K. P. and Jones, D. L. 2007. Evaluating the growth characteristics of lettuce in vermicompost and green waste compost. Eur. J. Soil Biol. 43: 316-319.
Alikhani, H. and Mohammadi, L. 2008. A comparison of physical and chemical properties of vermicompost and cold-compost and the effect of their use on tomato growth indicators. JAST. 39(1): 201-207. https://doi.org/10.1016/j.ejsobi.2007.08.045.
Benabderrahim, M. A., Elfalleh, W., Belayadi, H. and Haddad, M. 2018. Effect of date palm waste compost on forage alfalfa growth, yield, seed yield and minerals uptake. Int. J. Recycl. Org. 7: 1–9. https://doi.org/10.1007/s40093-017-0182-6.
Członka, S., Kairytė, A., Miedzińska, K. and Strąkowska, A. 2021. Polyurethane composites reinforced with walnut shell filler treated with perlite, Montmorillonite and Halloysite. Int. J. Mol. Sci. 22: 7304. https://doi.org/10.3390/ijms22147304.
Dhen, N., Abed, A. B., Zouba, S., Haouala, F. and AlMohandes, D. 2018. The challenge of using date branch waste as a peat substitute in container nursery production of lettuce (Lactuca sativa L.). Int. J. Recycl. Org. 7:357–364. https://doi.org/10.1007/s40093-018-0221-y.
Ebrahimi, R., Souri, M. K., Ebrahimi, F. and Ahmadizadeh, M. 2012. Growth and yield of strawberries under different potassium concentrations of hydroponic system in three substrates. World Appl. Sci. J, 16(10), pp.13801386.
Faostat, Crops, F. http://www.fao.org/faostat/en/# data. 2020.
Faostat, Crops: Production/Yield Quantities of Cucumbers and Gherkins in World. 2021.
Farrokhi, E., Samadi, A. and Rahimi, A. 2021. Evaluation of antioxidant activity, total phenol and flavonoid content of lemon balm (Melissa officinalis L.) in different cultures under hydroponic conditions. EJMP. 8(4): 19-33. magiran.com/p2259178.
Hammond, J. P. and White, P.J. 2008. Sucrose transport in the phloem: integrating root responses to phosphorus starvation. JXB. 59 (1): 93–109. https://doi.org/10.1093/jxb/erm221.
Ilahi, W. F. F. and Ahmad, D. 2017. A study on the physical and hydraulic characteristics of cocopeat perlite mixture as a growing media in containerized plant production. Sains Malaysiana. 46 (6):975-980. https://doi.org/ 10.17576/jsm-2017-4606-17.
Kamrani, M. H., Hashemimajd, K., Najafi, N. and Hoseinnia, H. 2014. Effect of planting bed and soilless media on growth and yield of potato minitubers. Agroecology Journal. 10(3): 25-35.
Kasahara, H. 2016. Current aspects of auxin biosynthesis in plants. Bioscience, Biotechnology, and Biochemistry. 80 (1): 34-42. https://doi.org/10.1080/09168451.2015.1086259.
Katsuhara, M., Otsuka, T. and Ezaki, B. 2005. Salt stress induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxidase is not enough for a recovery of root growth in arabidobsis. Plant Science. 169(2): 369-373. https://doi.org/10.1016/j.plantsci.2005.03.030.
Lemoine, R., Camera, S. L., Atanassova, R., Dédaldéchamp, F., Allario, T., Pourtau, N., Bonnemain, J. L., Laloi, M., Coutos-Thévenot, P., Maurousset, L., Faucher, M., Girousse, C., Lemonnier, P., Parrilla, J. and Maurousset, L. 2013. Source-to-sink transport of sugar and regulation by environmental factors. Front. Plant Sci. 4: 272. https://doi.org/10.3389/fpls.2013.00272.
Lichtenthaler, H. K. and Buschmann, C. 2001. Chlorophylls and carotenoids: Measurement and characterization by UV‐VIS spectroscopy. Curr. protoc. (3):821-828. https://doi.org/10.1002/0471142913.faf0403s01.
Lu, B., Wang, X., Liu, N. and Hu, C. 2021. Prediction performance optimization of different resolution and spectral band ranges for characterizing coco-peat substrate available nitrogen. JSS. 21: 2672–2685. https://doi.org/10.1007/s11368-021-02949-4.
Marchive, C., Roudier, F., Castaings, L., Bréhaut, V., Blondet, E., Colot, V., Meyer, C. and Krapp, A. 2013. Nuclear retention of the transcription factor NLP7 orchestrates the early response 702 to nitrate in plants. Nat Commun. 4: 1713. https://doi.org/ 10.1038/ncomms 2650.
Mazaheri Tirani, M., Madadkar Haghjou, M., Sulieman, S. and Ismaili, A. 2018. Comparative evaluation of zinc oxide effects on tobacco (Nicotiana tabacum L.) grown in different media. JAST. 20: 787-802. DOR: 20.1001.1.16807073.2018.20.4.11.9.
Michael, P. I. and Krishnaswamy, M. 2014. Membrane damage and activity of antioxidant enzymes in response to zinc and high irradiance stress in cowpea plant. IJCRR. 2: 112-128.
Mohammadi Ghehsareh, A. 2013. Effect of date palm wastes and rice hull mixed with soil on growth and yield of cucumber in greenhouse culture. Int. J. Recycl. Org. 2(17): 2–5. https://doi.org/10.1186/2251-7715-2-17.
Mohammadi Ghehsareh, A., Hematian, M. and Kalbasi, M. 2013. Comparison of date-palm wastes and perlite as culture substrates on growing indices in greenhouse cucumber. Int. J. Recycl. Org. 2(17):1-5. https://doi.org/10.1186/2251-7715-1-5.
Mohammadi Ghehsareh, A., Kalbasi, M. 2012. Effect of addition of organic and inorganic combinations to soil on growing property of greenhouse cucumber. AJB. 11(37). https://doi.org/10.5897/AJB11.1750.
Mohammadi Ghehsareh, A., Samad, N. and Borji, H. 2011. Comparison of date-palm wastes and perlite as growth substrates on some tomato growing indexes. African Journal of Biotechnology. 10(24): 4871-4878.
Nadarajah, K. K. 2020. ROS homeostasis in abiotic stress tolerance in plants. Int. J. Mol. Sci. 21: 5208. DOI: 10.3390/ijms21155208.
Rahbarian, P., Sardoei, A. S., Gholamshahi, A. S., Khorshidi, S. and Jandi, J. 2014. Relative water content, cell membrane stability, essential oil and morphology 50 of Dracocphalum moldavica L. are influenced by drought stress and manure, Department of Horticultural Sciences, MSc thesis, Islamic Azad University, Jiroft Branch, Iran. (In perssian).
Resende, M. L. V., Nojosa, G. B. A., Cavalcanti, L. S., Aguilar, M. A. G., Silva, L. H. C. P., Perez, J. O., Andrade, G. C. G., Carvalho, G. A. and Castro, R. M. 2002. Induction of resistance in cocoa against Crinipellis perniciosa and Verticillium dahliae by acibenzolar-S-methyl (ASM). Plant Pathology. 51: 621–8. https://doi.org/10.1046/j.1365-3059.2002.00754.x.
Sachdev, S., Ansari, S. A., Ansari, M. I., Fujita, M. and Hasanuzzaman, M. 2021. Abiotic stress and reactive oxygen species: generation, signaling, and defense mechanisms. Antioxidants. 10: 277. https://doi.org/10.3390/antiox10020277.
Shirani, M. and Mohammadi-Ghehsareh, A. 2013. Effect of date-palm waste as culture media on the growth of tomato. AJBAS. 7(8): 29-32. ISSN 1991-8178.
Singh, A. and Singh, G.S. 2017. Vermicomposting: A sustainable tool for environmental equilibria. Environ. Qual. Manag. 27: 23–40. https://doi.org/10.1002/tqem.21509.
Tirani, M. and Haghjou, M. 2019. Reactive oxygen species (ROS), total antioxidant capacity (AOC) and malondialdehyde (MDA) make a triangle in evaluation of zinc stress extension. JAPS. 29:1100-1111.
Vogt, T. 2010. Phenylpropanoid biosyntesis. J. Plant Mol. 3: 2-20-29. https://doi.org/10.1093/mp/ssp106.