Life-Stage-Dependent Secondary Responses of Some Biorational Insecticides on Trichogramma dendrolimi (Matsumura) (Hymenoptera: Trichogrammatidae)

Document Type : Original Research

Authors
Cansu Kandil
Hilal Tunca Cosic
Department of Plant Protection, Faculty of Agriculture, Ankara University, Ankara, Türkiye.
Abstract
In this study, lethal and sub-lethal doses of Neem Azal (azadirachtin 10 g L-1), Nimiks (azadirachtin 40 g L-1), Nimbecidine (azadirachtin 0.3 g L-1), Oread (spinosad 480 g L-1), and Nostalgist BL (1.5% Beauveria bassiana strain Bb-1-) were tested on different developmental stages of the egg parasitoid, Trichogramma dendrolimi, under laboratory conditions. The lowest melanized egg were found on the larval stage of parasitoid in the 200 mL dose of Nimiks (79.17%), on 5 mL dose of Oread (75.25%) and on 250 mL dose of Nimbecidine (79.37%). An approximately 10-fold decrease in emergence rates was determined in the larval, prepupal and pupal stages of the parasitoid at doses of 5 mL and 6.25 mL of Oread. The other doses of the same insecticides resulted in 100% mortality. The longest development time of T. dendrolimi was found on Oread with 6.25 mL (11.00 days), on Nimiks with 200 mL (11.04 days), and on Oread with 5 mL (10.90 days). No significant difference was observed in the sex ratio. The longevity of T. denrolimi was shorter than that of the control for all insecticides and doses applied to the larval, prepupal, and pupal stages of the parasitoid. Parasitism rates of F1 and F2 varied greatly depending on the insecticides, doses, and biological stage of the parasitoid. The new crop protection strategies aim to reduce the use of chemical insecticides while supporting the combined use of biorational insecticides and natural enemies. The study offers helpful data for IPM that is focused on the ecology.

Keywords

1.       Al-Deghairi, M. A. 2008. Bioassay Evaluation of the Entomopathogenic Fungi, Beauveria bassaina Vuellemin against Eggs and Nymphs of Bemisia tabaci Gennadius (Homoptera: Aleyrodidae). Pak. J. Biol. Sci., 11:1551-1560.
2.       Araujo, E. S., Poltronieri, A. S., Poitevin, C. G., Mirás-Avalos, J. M., Zawadneak, M. A. C. and Pimentel, I. C. 2020. Compatibility between Entomopathogenic Fungi and Egg Parasitoids (Trichogrammatidae): A Laboratory Study for Their Combined Use to Control Duponchelia fovealis. Insects, 11(9): 630.
3.       Aribi, N., Oulhaci, M. C., Kilani-Morakchi, S., Sandoz, J. C., Kaiser, L., Denis, B. and Joly, D. 2017. Azadirachtin Impact on Mate Choice, Female Sexual Receptivity and Male Activity in Drosophila melanogaster (Diptera: Drosophilidae). Pestic. Biochem. Physiol., 143: 95–101.
4.       Asma, C., Ons, I., Sabrine, B. A. and Kaouthar, L. G. 2018. Life-Stage-Dependent Side Effects of Selected Insecticides on Trichogramma cacoeciae (Marchal) (Hymenoptera: Trichogrammatidae) under Laboratory Conditions. Phytoparasitica, 46:105–113.
5.       Beloti, V. H., Alves, G. R., Araújo, D. F. D., Picoli, M. M., Moral, R. D. A., Demétrio, C. G. B. and Yamamoto, P. T. 2015. Lethal and Sublethal Effects of Insecticides Used on Citrus, on the Ectoparasitoid Tamarixia radiata. PLoS ONE, 10(7): e0132128.
6.       Consoli, F. L., Parra J. R. P. and Hassan, S. A. 1998. Side-Effects of Insecticides Used in Tomato Fields on the Egg Parasitoid Trichogramma pretiosum Riley (Hym., Trichogrammatidae), a Natural Enemy of Tuta absoluta (Meyrick) (Lep., Gelechiidae). J. Appl. Entomol., 122: 43-47.
7.       Guo, X., Di, N., Chen, X., Zhu, Z., Zhang, F. Tang, B., Dai, H., Li, J., Guo, R. and Wang, S. 2019. Performance of Trichogramma pintoi When Parasitizing Eggs of the Oriental Fruit Moth Grapholita molesta. Entomol. Gen., 3-4:239–249.
8.       Hamada, R. 1992. Egg Parasitoids of Common Cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Jpn. J. Appl. Entomol. Z, 36(4): 258-259.
9.       Kandil C., 2022. Researches on the Effect of Some Biorational Preparations on the Biological Characteristics of Trichogramma dendrolimi (Matsumura) (Hymenoptera: Trichogrammatidae). Department of Plant Protection, Graduate School of Natural and Applied Sciences, Ankara University.
10.    Kapoor, B. and K. Sharma. 2020. Biorational Pesticides: An Envirosafe Alternative to Pest Control. Indian Farmer, 7(08): 722-731.
11.    Li, L. Y. 1994. Worldwide Use of Trichogramma for Biological Control on Different Crops: A Survey. In: “Biological Control with Egg Parasitoids”, (Eds.): Wajnberg, E. and Hassan, S. A. CAB International, Wallingford, PP. 37–53.
12.    Lu, X., Han, S., Li, J., Liu, J. and Lia, Z. 2019. Effects of Cold Storage on the Quality of Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) Reared on Artificial Medium. Pest Manag. Sci., 75: 1328–1338.
13.    Lyons, D. B., Helson, B. V., Bourchier, R. S., Jones, G. C. and McFarlane, J. W. 2003. Effects of Azadirachtin-Based Insecticides on the Egg Parasitoid Trichogramma minutum (Hymenoptera: Trichogrammatidae). Can. Entomol., 135(5): 685-695. 10.4039/N02-113
14.    Mansour, R., Brévault, T., Chailleux, A., Cherif, A., Grissa-Lebdi, K. Haddi, Kh., Mohamed, S. A., Nofemela, R. S., Oke, A., Sylla, S., Tonnang, H. E. Z., Zappalà, L., Kenis, M., Desneux, N. and Biondi, A. 2018. Occurrence, Biology, Natural Enemies and Management of Tuta absoluta in Africa. Entomol. Gen., 38:83–11.
15.    Martins, I. C. F., Silva, R. J., Alencar, J. R. D. C. C., Silva, K. P., Cividanes, F. J., Duarte, R. T., Agostini, L. T. and Polanczyk, R. A. 2014. Interactions between the Entomopathogenic Fungi Beauveria bassiana (Ascomycota: Hypocreales) and the Aphid Parasitoid Diaeretiella rapae (Hymenoptera: Braconidae) on Myzus persicae (Hemiptera: Aphididae). J. Econ. Entomol., 107(3): 933-938.
16.    Matter, M. M., Gesraha, M. A., Ahmed, A. A. I. and Farag, N. A. 2002. Impact of Neem and Chinaberry Fruits Extract on the Pest/Parasitoid (Pieris rapae / Hyposoter ebeninus) Interactions. J. Pest Sci., 75(1): 13-18.
17.    Mitchell, P. L., Gupta, R., Singh, A. and Kumar, P. 2004. Behavioral and Developmental Effects of Neem Extracts on Clavigralla scutellaris (Hemiptera: Coreidae) and Its Eggs parasitoid, Gryon fulviventre (Hymenoptera: Scelionidae). J. Econ. Entomol,, 97(3): 916-923.
18.    Orr, D. B., Boethel, D. J. and Layton, M. B. 1989. Effect of Insecticide Applications in Soybeans on Trissolcus basalis (Hymenoptera: Scelionidae). J. Econ. Entomol., 82 (4): 1078-1084.
19.    Potrich, M., Alves, L. F., Haas, J., Da Silva, E. R., Daros, A., Pietrowski, V. and Neves, P. M. 2009. Seletividade de Beauveria bassiana e Metarhizium anisopliae a Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae). Neotrop. Entomol., 38(6): 822-826.
20.    Ramos Aguila, L. C., Akutse, K. S., Ashraf, H. J., Bamisile, B. S., Lin, J., Dai, J., Wang, H. and Wang, L. 2021. The Survival and Parasitism Rate of Tamarixia radiata (Hymenoptera: Eulophidae) on Its Host Exposed to Beauveria bassiana (Ascomycota: Hypocreales). Agronomy, 11: 1496.
21.    Saber, M., Hejazi, M. J. and Hassan, S. A. 2004. Effects of Azadirachtin/Neemazal on Different Stages and Adult Life Table Parameters of Trichogramma cacoeciae (Hymenoptera: Trichogrammatidae). J. Econ. Entomol., 97(3): 905-910.
22.    Saljoqi, Ahmad-Ur-Rahman U., Nawaz, M., Farid, A. and Khan, A. 2012. Compatibility of Spinosad with Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae) in Integrated Pest Management of Sitotroga cereallela. Pak. J. Zool., 44(1): 133-139.
23.    Shoeb, M. A. 2010. Effect of some Insecticides on the Immature Stages of the Egg Parasitoid Trichogramma evanescens West. (Hym., Trichogrammatidae). Egypt. Acad. J. Biol. Sci., A Entomol. 3(1): 31-38.
24.    Silva, D. M. D. and Bueno, A. D. F. 2015. Organic Products Selectivity for Trichogramma pretiosum (Hymenoptera: Trichogrammatidae). Arq. Inst. Biol., 82:01-08.
25.    Takada, Y., Kawamura, S. and Takana, T. 2001. Effects of Various Insecticides on the Development of the Egg Parasitoid Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae). J Econ. Entomol. 94(6):1341-1343.
26.    Vinson, S. B., Greenberg, S. M., Liu, T. X., Rao, A. and Volosciuk, L. F. 2015. Biological Control of Pests Using Trichogramma: Current Status and Perspectives. ISBN: 9787568300582, Northwest A&F Univ. Press, Yangling, 496 PP.
27.    Volkmar, C., Schumacher, K. and Müller J. 2008. Impact of Low-input Pesticides Usage on Spider Communities with Special Regards to Accumulated Effects. Pesticides Beneficial Organisms IOBC/Wprs Bull, 35: 18-25.
28.   Williams, T., Valle, J. and Vinuela, E. 2003. Is the Naturally Derived Insecticide Spinosad® Compatible with Insect Natural Enemies? Biocontrol Sci. Techn., 13(5): 459-475.
29.   Zar, J. H.  1999.  Biostatistical Analysis, 4th Edn. Upper Saddle River, NJ: Prentice-Hall. 
Journal of Agricultural Science and Technology
Volume 28, Issue 1
January and February 2026
Pages 179-190