Variation of Yield, Morphological Traits, and Essential Oil in Populations of Five Species of Stachys L. in Iran

Document Type : Original Research

Authors
N. Razazi 1
A. A. Jafari 2
Z. Khodarahmpour 3
S. Sadat 1
1 Department of Genetics and Plant Breeding, Ahvaz Branch, Islamic Azad University, Ahvaz, Islamic Republic of Iran.
2 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Islamic Republic of Iran.
3 Department of Production and Plant Genetics, Shoushtar Branch, Islamic Azad University, Shoushtar, Islamic Republic of Iran.
10.22034/jast.25.5.1179
Abstract
The genus Stachys L., (Lamiaceae family) has several spicy and aromatic species. In order to investigate the diversity of aerial biomass, morphological traits, and essential oil yield, 47 populations out of five species including S. lavandulifolia, S. laxa, S. inflata, S. germanica and S. byzantina were evaluated during two years, at a research farm in Khorramabad, Iran. The traits such as plant height, leaf length, leaf width, stem number, stem diameter, vegetation cover, 1,000 seed weight, and aerial dry (DM) yield in all 47 populations were measured. The essential oil was extracted using the hydrodistillation method with a Clevenger-type apparatus. The essential oil compounds were detected using GC and GC/MS. Data were analyzed using nested ANOVA with Minitab16 software. The results showed that both S. germanica and S. byzantina had higher aerial biomass than the other species. The Saveh and Tehran populations of S. inflata, the populations of Qazvin and Qorveh in S. lavandulifolia, and the populations of Ardebil and Semirom in S. Byzantine were superior to the others for the measured traits. For essential oil yields (w/w), the highest and lowest values were 2.0 and 0.8%, obtained in S. byzantina and S. lavandulifolia, respectively. Phytochemical analyses of S. lavandulifolia identified 43 compounds. The main compounds were β-eudesmol (3.48%), germacrene-D (4.59%), δ-cadinol (4.69%), bicyclogermacrene (6.85%), δ-cadinene (9.69%), spathulenol (10.08%) and α-cadinol (12.86%). S. lavandulifolia, with early maturity and higher essential oil, was recommended for domestication and breeding of improved varieties.

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Aghaei Noroozloo, Y., Mirjalili, M. H., Nazeri, V. and Moshrefi Araghi, A. 2015. Evaluation of some ecological factors, morphological traits and essential oil productivity of Stachys lavandulifolia Vahl. in four provinces of Iran. Iran. J. Med. Arom. Plant, 30(6): 985-998.
Aharizad, S., Rahimi, MH., Toorchi, M., Mohebalipour, N. 2013. Assessment of the relationship between effective traits on yield and the astral part of lemon balm (Melissa officinalis L.) populations using path analysis. Indian J. Sci. Tech. 6(5): 4447-4452.
Andi, SA., Nazeri, V., Zamani, Z., Hadian, J. 2011. Morphological diversity of wild Origanum vulgare (Lamiaceae) in Iran. Iran. J. Bot. 17: 211-221.
Arabsalehi, F., Rahimmalek, M., Ehtemam; MH. and Salehi, A. 2016. Assessment of genetic variation in different Stachys lavandulifolia accessions using morphological and essential oil content. Taxon. Biosys. J. 8: 41-50.
Bhattacharjee, R. 1980. Taxonomic studies in Stachys II: A new infrageneric classification of Stachys L. Notes Royal Bot Garden Edinburgh, UK, 38: 65-96.
Chorli, S., Khorasaninejad, S., Hemmati, K. and Kashefi, B. 2017. The study of morphological characteristics, antioxidant and essential oil contents of the medicinal plant Stachys lavandulifolia Vahl. in habitats of Semnan, Razavi, and North Khorasan provinces. J. Plant Environ. Physiol. 41: 41-52.
Feizbaksh, A., Tehrani M.S and Rustaiyan, A. and Masoudi, S. 2003. Composition of the essential oil of Stachys lavandulifolia Vahl. from Iran. J. Essential Oil Res. 15(2): 72-73.
Hosseini Mazinani, M., Tajali, A., Gandomkar, A., & Roshandelpour, A. 2013. Variability in chemical constituent of the essential oil of two species of Stachys genus from Iran. Int. J. Agri. Crop Sci. 5: 2773-2776.
Hungarian pharmacopoeia. 1984. VII Kiadas, Kotet, Medicine Publication, Hungary. 918 p.
Karimi, E., Ghasemnejad, A., Hadian, J., Akhundi, R., Ghorbanpour, M. 2014. Evaluation of morphological diversity and essential oil yield of Satureja mutica Fisch. and CA Mey. populations growing wild in Iran. J. Horti. Forest. Biotech. 18(1): 7-16.
Keshavarzi, M., Rezaei, M., Miri, M. 2016. The comparison of morphological and phytochemical evaluation in some population of Stachys lavandulifolia Vahl. in different provinces under field conditions. Eco-phytochem. J. med. Plants, 4 (2): 78-87.
Kharazian, N. 2012. Morphometric study of some Salvia L. (Lamiaceae) species in Iran. Sci. J. Bio. Sci. 1(6): 126-137.
Kusano, T., Berberich, T., Tateda, C. and Takahashi, Y. (2008). Polyamines: essential factors for growth and survival. J. Planta, 228: 367-381.
Martin, E., Çetin Ö., Akçiçek, E. and Dirmenci, T. 2011. New chromosome counts of genus Stachys (Lamiaceae) from Turkey. Turkish J. Bot. 35: 671-680.
Mirzaee Nodoshan, H., Mehrpor, Sh., Sefidkan, F. 2006. Analysis of causality of effective traits in essential oil in 3 Thyme species. Res. Const. J. 70: 88-94.
Nejadhabibvash, F., Rezaee, M.B., Mahmudi, A. and Jaimand, K. 2018. Effect of Harvesting Time on Content and Chemical Composition of Essential Oil from Stachys lavandulifolia Vahl (Lamiaceae). J. Med. Plants and By-Products, 2: 181-187.
Rahimmalek, M., Bahreininejad, B., Khorami, M. and Sayed Tabatabaei, B.E. 2009. Genetic diversity and geographical differentiation of Thymus daenensis, an endangered medicinal plant, as revealed by Inter Simple Sequence Repeat (ISSR) markers. Biochem. Genet. 47: 831–842.
Rakover, Y., Ben-Arye, E. and Goldstein, LH. 2008. The treatment of respiratory ailments with essential oils of some aromatic medicinal plants. Harefuah, 147: 783-788.
Rechinger KH. 1982. Stachys. In: Rechinger KH, editor. Flora Iranica Iran: NHBS.;354-396.
Sarwari, A., Dianti Tilki, A., Rezaei, M.; Zadbar, M. 2015. The effect of some environmental factors on the quantity and quality of S. lavandolifulia essential oil in Khorasan Razavi province (Chenaran). Ecophytochem. Med. Plants, 2: 1-7.
Tabaei-Aghdaei, S.R., Abootorabi Najafabadi, M., Lebaschi, M.H., Najafi-Ashtiani, A., Jafari, A.A., Sefidkon F. and Mirjani, L. 2018. Yield quantity and quality of Satureja spicigera (C. Koch) Boiss. and S. sahendica Bornm. accessions under dry farming condition in Damavand. Iran. J. Med. Arom. Plants, 34 (2): 183-192.
Tomou, E.M., Barda, C. and Skaltsa. H. 2020. Genus Stachys: A Review of Traditional Uses, Phytochemistry and Bioactivity Medicines, 7(10): 63.
Yousefi, Azar, V. 2017. Investigation of genetic diversity of some Salvia species using molecular marker. The 7th Horticulture sciences conference, Iran, pp, 726-728.
Journal of Agricultural Science and Technology
Volume 25, Issue 5
September and October 2023
Pages 1179-1191