Effect of Exogenous Phenolic Substances on Susceptibility of Octoploid Strawberry to Crown Rot

Document Type : Original Research

Authors
C. Luo
Y. Hu
B. Shu
College of Horticulture and Gardening, Yangtze University, 434025 Jingzhou, P. R. China.
10.22034/jast.25.3.673
Abstract
Crown rot caused by Colletotrichum siamense is a serious disease of strawberry in the Yangtze River region, China. The metabolites involved in phenylpropanoid biosynthesis increase susceptibility of crown rot in octoploid strawberry. Exogenous coumaric acid, caffeic acid, and ferulic acid involved in phenylpropanoid biosynthesis were used to explore whether the increased susceptibility was associated with reactive oxygen species, antioxidant substance and key genes of phenylpropanoid metabolism. According to the results, H2O2, O2.−, and MDA contents showed different responses to C. siamense infection in root, petiole and leaf. The H2O2, O2.− and MDA were increased by C. siamense in petiole. Exogenous coumaric acid, caffeic acid and ferulic acid promoted the H2O2, O2.− and MDA as positive control. The POD activity was significantly induced in response to C. siamense infection in petiole. The coumaric acid treatment inhibited the POD activity but increased total phenolics. The ferulic acid only promoted POD activity in petiole. In addition, the expressions of transcripts involved in phenylpropanoid biosynthesis were regulated by exogenous coumaric acid, caffeic acid, and ferulic acid. Together, our results demonstrated that exogenous coumaric acid, caffeic acid, and ferulic acid increased susceptibility of octoploid strawberry to crown rot by regulated ROS, antioxidant substance, and transcripts expression of phenylpropanoid biosynthesis.

Keywords

Subjects

1. Camejo, D., Martí, M. C., Jiménez, A., Cabrera, J. C., Olmos, E., and Sevilla, F. 2011. Effect of Oligogalacturonides on Root Length, Extracellular Alkalinization and O2−-Accumulation in Alfalfa. J. Plant physiol., 168: 566–575.
2. Dangl, J. L., and Jones, J. D. 2001. Plant Pathogens and Integrated Defence Responses to Infection. Nature, 411: 826–833.
3. de Freitas-Silva, L., Rodríguez-Ruiz, M., Houmani, H., da Silva, L. C., Palma, J. M., and Corpas, F. J. 2017. Glyphosate-Induced Oxidative Stress in Arabidopsis thaliana Affecting Peroxisomal Metabolism and Triggers Activity in the Oxidative Phase of the Pentose Phosphate Pathway (OxPPP) Involved in NADPH Generation. J. Plant Physiol., 218: 196–205.
4. Deng, J., Bi, Y., Zhang, Z., Xie, D., Ge, Y., Li, W., Wang, J., Wang, Y. 2015. Postharvest Oxalic Acid Treatment Induces Resistance Against Pink Rot by Priming in Muskmelon (Cucumis melo L.) Fruit. Postharvest Biol. Technol. 106: 53–61.
5. Desmedt, W., Jonckheere, W., Nguyen, V. H., Ameye, M., De Zutter, N., De Kock, K., Debode, J., Van Leeuwen, T., Audenaert, K., Vanholme, B., and Kyndt, T. 2021. The Phenylpropanoid Pathway Inhibitor Piperonylic Acid Induces Broad‐Spectrum Pest and Disease Resistance in Plants. Plant Cell Environ., 44: 3122–3139
6. Gilroy, S., Suzuki, N., Miller, G., Choi, W. G., Toyota, M., Devireddy, A. R., and Mittler, R. 2014. A Tidal Wave of Signals: Calcium and ROS at the Forefront of Rapid Systemic Signaling. Trends Plant Sci., 19: 623–630.
7. He, J. D., Zou, Y. N., Wu, Q. S., and Kuča, K. 2020. Mycorrhizas Enhance Drought Tolerance of Trifoliate Orange by Enhancing Activities and Gene Expression of Antioxidant Enzymes. Sci. Hortic.-AMSTERDAM, 262: 108745.
8. Kumar, N., Prabhu, P. A. J., Pal, A. K., Remya, S., Aklakur, M., Rana, R. S., Gupta, S., Raman R. P., and Jadhao, S. B. 2011. Anti-Oxidative and Immuno-Hematological Status of Tilapia (Oreochromis mossambicus) during Acute Toxicity Test of Endosulfan. Pestic. Biochem. Phys., 99: 45–52.
9. Li, G., Zhu, S., Wu, W., Zhang, C., Peng, Y., Wang, Q., Shi, J. 2017. Exogenous Nitric Oxide Induces Disease Resistance Against Monilinia fructicola through Activating the phenylpropanoid Pathway in Peach Fruit. J. Sci. Food Agric., 97: 3030–3038.
10. Li, X., Lin, J., Gao, Y., Han, W., and Chen, D. 2012. Antioxidant Activity and Mechanism of Rhizoma Cimicifugae. Chem. Cent. J., 6: 1–10.
11. Luo, C., Hu, Y. Y., and Shu, B. 2021. Characterization of Colletotrichum siamense Causing Crown Rot of Strawberry in Jingzhou, Hubei Province. Not. Bot. Horti. Agrobo., 49: 1–13.
12. Luo, C., Sun, Q., Zhang, F., Zhang, D., Liu, C., Wu, Q., and Shu, B. 2020. Genome-Wide Identification and Expression Analysis of the Citrus Malectin Domain-Containing Receptor-Like Kinases in Response to Arbuscular Mycorrhizal Fungi Colonization and Drought. Hortic. Environ. Biote., 61: 891–901.
13. Ma, W., Li, J., Murtaza, A., Iqbal, A., Zhang, J., Zhu, L., Xu, X. Pan, S., and Hu, W. 2022. High-Pressure Carbon Dioxide Treatment Alleviates Browning Development by Regulating Membrane Lipid Metabolism in Fresh-Cut Lettuce. Food Control, 134: 108749.
14. Matern, U., and Kneusel, R.E., 1988. Phenolic Compounds in Plant Disease Resistance. Phytoparasitica, 16: 153–170.
15. Mellersh, D. G., Foulds, I. V., Higgins, V. J., and Heath, M. C. 2002. H2O2 Plays Different Roles in Determining Penetration Failure in Three Diverse Plant–Fungal Interactions. Plant J., 29: 257–268.
16. Nath, M., Bhatt, D., Prasad, R., and Tuteja, N. 2017. “Reactive oxygen species (ROS) Metabolism and Signaling in Plant-Mycorrhizal Association under Biotic and Abiotic Stress Conditions,” in Mycorrhiza- Eco-Physiology, Secondary Metabolites, Nanomaterials. eds. A. Varma, R. Prasad and N. Tuteja (Cham: Springer), 223–232.
17. Nathan, C., and Cunningham-Bussel, A. 2013. Beyond Oxidative Stress: An Immunologist's Guide to Reactive Oxygen Species. Nat. Rev. Immunol., 13: 349–361.
18. Oliveira, M. D. M., Varanda, C. M. R., and Félix, M. R. F. 2016. Induced Resistance During the Interaction Pathogen X plant and the use of resistance inducers. Phytochem. Lett., 15: 152–158.
19. Ray, P. D., Huang, B. W., and Tsuji, Y. 2012. Reactive Oxygen Species (ROS) Homeostasis and Redox Regulation in Cellular Signaling. Cell Signal., 24: 981–990.
20. Rodrigues, R. C., Ortiz, C., Berenguer-Murcia, Á., Torres, R., and Fernández-Lafuente, R. 2013. Modifying Enzyme Activity and Selectivity by Immobilization. Che. Soc. Rev., 42: 6290–6307.
21. Sharma, P., Jha, A. B., Dubey, R. S., and Pessarakli, M. 2012. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. J. Botany, 2012: 217037
22. Shetty, N. P., Kristensen, B. K., Newman, M. A., Møller, K., Gregersen, P. L., and Jørgensen, H. L. (2003). Association of Hydrogen Peroxide with Restriction of Septoria Tritici in Resistant Wheat. Physiol. Mol. Plant P., 62: 333–346.
23. Sudhakar, C., Lakshmi, A., and Giridarakumar, S. 2001. Changes in the Antioxidant Enzyme Efficacy in Two High Yielding Genotypes of Mulberry (Morus alba L.) under NaCl Salinity. Plant Sci., 161: 613–619.
24. Suzuki, N., Miller, G., Morales, J., Shulaev, V., Torres, M. A., and Mittler, R. 2011. Respiratory Burst Oxidases: the Engines of ROS Signaling. Curr. Opin. Plant Biol., 14: 691–699.
25. Velikova, V., Yordanov, I., and Edreva, A. 2000. Oxidative Stress and Some Antioxidant Systems in Acid Rain-Treated Bean Plants: Protective Role of Exogenous Polyamines. Plant Sci., 151: 59–66.
26. Vivancos, P. D., Dong, Y., Ziegler, K., Markovic, J., Pallardó, F. V., Pellny, T. K., Verrier, P. J., and Foyer, C. H. 2010. Recruitment of Glutathione into the Nucleus During Cell Proliferation Adjusts Whole‐Cell Redox Homeostasis in Arabidopsis thaliana and Lowers the Oxidative Defence Shield. Plant J., 64: 825–838.
27. Vuleta, A., Jovanović, S. M., and Tucić, B. 2016. Adaptive Flexibility of Enzymatic Antioxidants SOD, APX and CAT to High Light STRess: The Clonal Perennial Monocot Iris Pumila as a Study Case. Plant Physiol. Bioch., 100: 166–173.
28. Wu, Q. S., Zou, Y. N., and Xia, R. X. 2006. Effects of Water Stress and Arbuscular Mycorrhizal Fungi on Reactive Oxygen Metabolism and Antioxidant Production by Citrus (Citrus tangerine) Roots. Eur. J. Soil Biol., 42: 166–172.
29. Yadav, V., Wang, Z., Wei, C., Amo, A., Ahmed, B., Yang, X., and Zhang, X. 2020. Phenylpropanoid Pathway Engineering: An Emerging Approach Towards Plant Defense. Pathogens, 9: 312.
30. Yong-Hong, G. E., Yang, B. I., Li, Y. C., and Wang, Y. 2012. Resistance of Harvested Fruits and Vegetables to Diseases Induced by ASM and Its Mechanism. Sci. Agri. Sinica., 16: 3357–3362.
31. Zhang, M., Wang, D., Gao, X., Yue, Z., and Zhou, H. 2020. Exogenous Caffeic Acid and Epicatechin Enhance Resistance Against Botrytis cinerea through Activation of the Phenylpropanoid Pathway in Apples. Sci. Hortic.-AMSTERDAM, 268: 109348.
32. Zhu, J. X., Wu, H., and Sun, Q. J. 2019. Preparation of Crosslinked Active Bilayer Film Based on Chitosan and Alginate for Regulating Ascorbate-Glutathione Cycle of Postharvest Cherry Tomato (Lycopersicon esculentum). Int. J. Biol. Macromol., 130: 584–594.
Journal of Agricultural Science and Technology
Volume 25, Issue 3
May and June 2023
Pages 673-685