Ecologically Friendly Formulations Based on Tebuconasole for Plant Protection and Their Biological Efficacy

Document Type : Original Research

Authors
S. Khalikov 1
O. I. Teplyakova 2
N. G. Vlasenko 2
O. Y. Selyutina 3
N. E. Polyakov 3
1 A. N. Nesmeyanov Institute of Organoelement Compounds, 28 Vavilova st., Moscow, 1119991, Russia.
2 Siberian Federal Scientific Centre of Agro-BioTechnologies, Krasnoobsk, Novosibirsk region, 630501, Russia.
3 V. V. Voevodsky Institute of Chemical Kinetics and Combustion, 3 Institutskaya St., Novosibirsk, 630090, Russia.
10.52547/jast.25.2.403
Abstract
Currently, nanotechnologies are being actively introduced into agriculture, in particular in the field of creating new effective plant protection products. This is achieved through the development of nanosized controlled release systems, such as polymer nanoparticles, micelles, and so on using a wide variety of materials. In the present study, we applied original approach based on “green” mechanochemical technology to prepare new nanocomposites of pesticide Tebuconazole (TBC) for treating wheat seeds against pathogenic microflora (B. sorokiniana, Fusarium spp., Alternaria spp., Penicillum spp.). The size distribution of nanoparticles for three TBC formulations (microcapsules, microemulsionsб nanosuspensions) was measured using dynamic light scattering technique. All formulations contained nanoparticles (10-300 nm) and we aimed to find the most suitable size for effective penetration into cell membranes. The narrowest size distribution (225±40 nm) was observed for nanosuspension based on Licorice Extract (LE). The microcapsules based on Na-CMC also contained micro-sized particles (1,500 nm), which are apparently aggregates of nanoparticles. The laboratory and field biological tests revealed a high activity of the developed formulations against all pathogenic microflora under study, with a low retardant effect. Nanosuspension is considered as the most “environmentally friendly preparation”, since it contains only natural LE as an adjunct. This formulation with a consumption rate of 0.25 Lt-1 suppressed 100% B. sorokiniana, Fusarium spp. and Penicillum spp. infections, possibly due to the presence of natural saponin glycyrrhizic acid, which interacts with plant membranes and promotes better penetration of TBC into the grain.

Keywords

Subjects

REFERENCES
1. Asrar, J., Ding, Y., La Monica, R.E. and Ness, L.C. 2004. Controlled release of tebuconazole from a polymer matrix microparticle: release kinetics and length of efficacy. J. Agric. Food Chem., 52: 4814–4820.
2. Cai, J., Luo, S., Li, X., Deng, Y., Huang, H., Zhao, B. and Li, G. 2019. Formulation of injectable glycyrrhizic acid-hydroxycamptothecin micelles as new generation of DNA topoisomerase I inhibitor for enhanced antitumor activity. Int. J. Pharm., 571:118693.
3. Díaz-Blancas, V., Medina, D.I., Padilla-Ortega, E., Bortolini-Zavala, R., Olvera-Romero, M. and Luna-Bárcenas, G. 2016. Nanoemulsion formulations of fungicide tebuconazole for agricultural applications. Molecules, 21(10): 1271.
4. Hameed, A. and Farooq, T. 2021. Triazole-Based Plant Growth-Regulating Agents: A Recent Update, In: “Advances in Triazole Chemistry”, (Ed.): Farooq, T., Elsevier, PP. 169-185.
5. Kato, H., Nakamura, A., Ouchi, N., Kinugasa, S. 2016. Determination of bimodal size distribution using dynamic light scattering methods in the submicrometer size range. Materials Express, 6(2): 175-182.
6. Khalikov, S.S., Malyuga, A.A. and Chulikova, N.S. 2019. Complex preparations for the protection potato on the basis of tebuconazole. J. Agric. Sci. Tech. A, 9: 338-343.
7. Korsukova, A.V., Gornostai, T.G., Grabeinych, O.I., Dorofeev, N.V., Pobezhimova, T.P., Sokolova, N.A., Dudareva, L.V. and Voinikov, V.K. 2016. Tebuconazole regulates fatty acid composition of etiolated winter wheat seed lings. J. Stress Physiol. Biochem., 12 (2): 72–79.
8. Paranjape, K., Gowariker, V., Krishnamurthy, V.N. and Gowariker, S. 2014. The pesticide encyclopedia. CABI.
9. Pereira, A.E.S., Oliveira, H.C., Fraceto, L.F. and Santaella, C. 2021. Nanotechnology Potential in Seed Priming for Sustainable Agriculture- Review. Nanomaterials, 11: 267.
10. Scharma, K.K., Singh, U.S., Sharma, P. and Kumar, A. 2015. Seed treatments for sustainable agriculture-A review. J. Appl. Nat. Sci., 7(1): 521-539.
11. Selyutina, O.Yu., Polyakov, N.E., Korneev, D.V. and Zaitsev, B.N. 2014. Influence of glycyrrhizin on permeability and elasticity of cell membrane: perspectives for drugs delivery. Drug Deliv., 23: 1–8
12. Selyutina, O.Yu., Apanasenko, I.E., Kim, A.V., Shelepova, E.A., Khalikov, S.S. and Polyakov, N.E. 2016. Spectroscopic and molecular dynamics characterization of glycyrrhizin membrane-modifying activity. Colloids Surf. B, 147: 459–466.
13. Selyutina, O.Yu., Apanasenko, I.E., Khalikov, S.S. and Polyakov, N.E. 2017. Natural poly- and oligosaccharides as novel delivery systems for plant protection compounds. J. Agric. Food Chem., 65(31): 6582–6587.
14. Selyutina, O.Yu. and Polyakov, N.E. 2019. Glycyrrhizic acid as a multifunctional drug carrier - From physicochemical properties to biomedical applications: A modern insight on the ancient drug. Int. J. Pharm., 559: 271–279.
15. Selyutina, O.Yu., Khalikov, S.S. and Polyakov, N.E. 2020. Arabinogalactan and glycyrrhizin based nanopesticides as novel delivery systems for plant protection. Envir. Sci. Poll. Res., 27: 5864–5872.
16. Vlasenko, N.G., Khalikov, S.S. and Burlakova, S.V. 2020. Flexibile Techhology of Protectants for Grain Seeds. IOP Conf. Ser. Earth Environ. Sci., 548: 082003.
17. Volova, T.G., Prudnikova, S.V., Zhila, N.O.,Vinogradova, O.N., Shumilova, A.A., Nikolaeva, E.D., Kiselev, E.G. and Shishatskaya, E.I. 2017. Efficacy of tebuconazole embedded in biodegradable poly-3-hydroxybutyrate to inhibit the development of Fusarium moniliforme in soil microecosystems. Pest Manag. Sci., 73(5): 925–935.
18. Volova, T.G., Prudnikova, S.V. and Zhila, N.O. 2018. Fungicidal activity of slow-release P(3HB)/TEB formulations in wheat plant communities infected by Fusarium moniliforme. Envir. Sci. Poll. Res., 25(1): 552–561.
19. Xiao, J., Chen, L., Pan, F., Deng, Y., Ding, C., Liao, M. and Cao, H. 2020. Application method affects pesticide efficiency and effectiveness in wheat fields. Pest Manag. Sci., 76(4):1256-1264.
20. Yang, F.H., Zhang, Q., Liang, Q.Y., Wang, S.Q., Zhao, B.X., Wang, Y.T., Cai, Y. and Li, G.F. 2015 Bioavailability enhancement of paclitaxel via a novel oral drug delivery system: paclitaxel-loaded glycyrrhizic acid micelles. Molecules, 20: 4337–4356.
21. Zhang, S., Yang, X., Tu, Z., Hua, W., He, P., Li, H. and Ren, T. 2020. Influence of the hydrophilic moiety of polymeric surfactant on their surface activity and physical stability of pesticide suspension concentrate. J. Mol. Liq., 317: 114136.
Journal of Agricultural Science and Technology
Volume 25, Issue 2
January and February 2023
Pages 403-414