Rapid Determination of Ethanol in Non-Alcoholic Malt Beverage by ATR-FT-IR Spectroscopy and Headspace Gas Chromatography Confirmation

Document Type : Original Research

Authors
F. Zamani Mazdeh 1
A. Chalipour 2
F. Salami 1
M. Amini 3
H. Adli 1
A. Rostami 1
S. Rashidi Germi 2
M. Nobahari Quchan Atigh 1
M. Hajimahmoodi 1
1 Drug and Food Control Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
2 Food and Drug Administration, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
3 Department of Medical Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran.
10.52547/jast.25.2.391
Abstract
Malt beverage is one of the most popular drinks in the world. Recently, consumption of non-alcoholic beverages has expanded significantly in many countries. The permitted level of alcohol in malt beverage is less than 0.5% by Iranian National Standards. In this study, a method was developed to determine ethanol content in malt beverages by FTIR, equipped with Horizontal Attenuated Total Reflectance (H-ATR). Here, the Limit Of Detection (LOD) and Limit Of Quantification (LOQ) were 0.07% and 0.23%, respectively. The correlation coefficient of calibration curve was higher than 0.999. Fifty commercial malt beverages from six brands (five Iranian and one imported brand) and three types of flavored malt beverage (classic, equatorial, and lemon) were assessed. The average detected ethanol amount in samples was 0.19%, varying between 0.00-1.47percent. The results showed that the amount of ethanol in lemon malt drinks was more than the maximum permitted limit; that could be attributed to the usage of ethylene glycol for extraction of lemon flavor.

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Subjects

1169/2011 CrEN, regarding the mandatory labelling of the list of ingredients and the nutrition declaration of alcoholic beverages 2017.
Abdel-Banat BM, Hoshida H, Ano A, Nonklang S, Akada R 2010, High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?, Appl. Microbiol. Biotechnol 85:861-867.
Alzeer J, Hadeed KA 2016, Ethanol and its Halal status in food industries, Trends in food science & technology 58:14-20.
Anis Najiha A, Tajul A, Norziah M, Wan Nadiah W 2010, A preliminary study on Halal limits for ethanol content in food products, Middle east journal of scientific research 6:45-50.
Belghith H, Romette JL, Thomas D 1987, An enzyme electrode for online determination of ethanol and methanol, Biotechnology and bioengineering 30:1001-1005.
Brill SK, Wagner MS 2012, Alcohol determination in beverages using polar capillary gas chromatography-mass spectroscopy and an acetonitrile internal standard, Concordia college journal of analytical chemistry 3:6-12.
Chu W, Echizen T, Kamiya Y, Okuhara T 2004, Gas-phase hydration of ethene over tungstena–zirconia, applied catalysis A: General 259:199-205.
Davies A, Grant A 1988, Near infrared spectroscopy for the analysis of specific molecules in food Analytical applications of spectroscopy, edited by CS Creaser and AMC Davies.
Duarte IF, Barros A, Almeida C, Spraul M, Gil AM 2004, Multivariate analysis of NMR and FTIR data as a potential tool for the quality control of beer, Journal of agricultural and food chemistry 52:1031-1038.
El-Naggar A, Arida H, Montasser M, Hassan R 2012, Measures affecting alcohol in malt beverages according to Islamic religion, J am sci 8:455-460.
Elmer P 2005, FT-IR Spectroscopy–Attenuated total reflectance (ATR) Technical note.
Erdei B, Hancz D, Galbe M, Zacchi G 2013, SSF of steam-pretreated wheat straw with the addition of saccharified or fermented wheat meal in integrated bioethanol production, Biotechnology for biofuels 6:169.
Esti M, Volpe G, Compagnone D, Mariotti G, Moscone D, Palleschi G 2003, Monitoring alcoholic fermentation of red wine by electrochemical biosensors, American journal of enology and viticulture 54:39-45.
Hosseini E, Kadivar M, Shahedi M 2011, Physicochemical properties and storability of Non-alcoholic malt drinks prepared from oat and barley malts, Journal of agricultural science and technology 14:173-182.
Iranian national standard (ISIRI) [Internet] 2011, Iran: Iranian national standard NO.2279 . 5th ED. Available from: .http://www.isiri.org/portal/files/std/2279.pdf
Li G, Yan N, Yuan L, Wu J, Du J, Gao YE, Peng Y 2020, Rapid Analysis of Alcohol Content During the Green Jujube Wine Fermentation by FT-NIR, E3S Web of Conferences 145:01037.
Maldonado-Devincci AM, Badanich KA, Kirstein CL 2010, Alcohol during adolescence selectively alters immediate and long-term behavior and neurochemistry, Alcohol 44:57-66.
Mansur AR, Oh J, Lee HS, Oh SY 2022, Determination of ethanol in foods and beverages by magnetic stirring-assisted aqueous extraction coupled with GC-FID: A validated method for halal verification, Food Chem 366:130526.
Meyer JS, Quenzer LF 2013, Psychopharmacology: Drugs, the brain, and behavior.
Moshonas MG, Shaw PE 1972, Analysis of flavor constituents from lemon and lime essence, Journal of agricultural and food chemistry 20:1029-1030.
Nagarajan R, Gupta A, Mehrotra R, Bajaj M 2006, Quantitative analysis of alcohol, sugar, and tartaric acid in alcoholic beverages using attenuated total reflectance spectroscopy, Journal of analytical methods in chemistry.
Nordon A, Mills A, Burn RT, Cusick FM, Littlejohn D 2005, Comparison of non-invasive NIR and Raman spectrometries for determination of alcohol content of spirits, Anal. Chim. Acta 548:148-158.
Onuki S, Koziel JA, van Leeuwen J, Jenks WS, Grewell D, Cai L 2008, Ethanol production, purification, and analysis techniques: a review, ASABE, paper.
Riaz MN, Chaudry MM 2003, Halal food production, CRC press.
Rocchia M, Rossi A, Zeppa G 2007, Determination of ethanol content in wine through a porous silicon oxide microcavity, Sensors and Actuators B: Chemical 123:89-93.
Somboon T, Phatchana R, Tongpoothorn W, Sansuk S 2022, A simple and green method for determination of ethanol in liquors by the conductivity measurement of the uncatalyzed esterification reaction, LWT--Food Sci. Technol 154:112593.
Stuart B 2005, Infrared spectroscopy, Wiley online library.
Stupak M, Kocourek V, Kolouchova I, Hajslova J 2017, Rapid approach for the determination of alcoholic strength and overall quality check of various spirit drinks and wines using GC–MS, Food control 80:307-313.
Thungon PD, Kakoti A, Ngashangva L, Goswami P 2017, Advances in developing rapid, reliable and portable detection systems for alcohol, Biosensors and Bioelectronics 97: 83-99.
Van der Spiegel M, van der Fels-Klerx HJ, Sterrenburg P, Van Ruth SM, Scholtens-Toma IMJ, Kok EJ 2012, Halal assurance in food supply chains: Verification of halal certificates using audits and laboratory analysis, Trends in food science & technology 27:109-119. doi:http://dx.doi.org/10.1016/j.tifs.2012.04.005.
Varlet V, Augsburger M 2013, Confirmation of natural gas explosion from methane quantification by headspace gas chromatography–mass spectrometry (HS-GC-MS) in postmortem samples: A case report. International journal of legal medicine 127:413-418.
Vijayalaxmi S, Anu Appaiah KA, Jayalakshmi SK, Mulimani VH, Sreeramulu K 2013, Production of bioethanol from fermented sugars of sugarcane bagasse produced by lignocellulolytic enzymes of exiguobacterium sp. VSG-1, Applied biochemistry and biotechnology 171:246-260. doi:10.1007/s12010-013-0366-0.
Wachełko O, Szpot P, Zawadzki M 2021, The application of headspace gas chromatographic method for the determination of ethyl alcohol in craft beers, wines and soft drinks, Food Chem 346:128924.
Yilmaz SGH, Goren AC 2013, Halal food and metrology: Ethyl alcohol contents of beverages, Journal of chemical metrology 7:7.
Yue H, Ma X, Gong J 2014, An alternative synthetic approach for efficient catalytic conversion of syngas to ethanol, Accounts of chemical research 47:1483-1492.
Journal of Agricultural Science and Technology
Volume 25, Issue 2
January and February 2023
Pages 391-401