Investigation of Antioxidant, Antimicrobial and Anticancer Potential of Silver Nanoparticles Synthesized by Viola tricolor L. Extract

Document Type : Original Research

Authors
A. Hassanvand 1
S. Saadatmand 1
H. Lari Yazdi 2
A. R. Iranbakhsh 1
1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2 Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Islamic Republic of Iran
Abstract
Silver Nanoparticles (AgNPs) are widely used in medical and pharmaceutical applications due to their antimicrobial properties. In this study, Ag-NPs were synthesized using Viola tricolor aqueous extract as a reducing and capping agent. The presence of synthesized Ag-NPs was first confirmed with UV-Visible, SEM, TEM, XRD, and FTIR analyses, and then their antimicrobial characteristics were studied based on the Minimum Inhibitory Concentration (MIC). The SEM analysis showed that the synthesized AgNPs were spherical in shape. The particle size histogram revealed that the average particle size of the AgNPs was 49.45 nm. Findings from the FTIR and UV-Vis spectra showed the successful formation of Ag-NPs because the functional groups involved in the synthesis process and adsorption peaks were well developed. Furthermore, the Ag-NPs had a peak absorption at 420 nm in the spectrometry. MIC results showed the strong antimicrobial effects of the synthesized Ag-NPs. Results of the Minimum Bactericidal Concentration (MBC) revealed the dose-dependent cytotoxicity of the Ag-NPs. Nanoparticles could exert the inhibitory effect of DDPH free radicals in a dose-dependent manner. Methyl tetrazolium (MTT) results showed that silver nanoparticles had a dose-dependent cytotoxic effect and significantly reduced cell survival. The IC50 values for Ag-NPs and the extract were 11.83 and 204.4 μg mL-1, respectively. This study showed a higher cytotoxic effect of the green synthesized nanoparticles on hepatocellular carcinoma cells than the extract. Consequently, the biosynthesis of Ag-NPs using aqueous extract of V. tricolor is clean, inexpensive, and has antibacterial, anticancer and antioxidant activities. Hence, it can be used as a drug candidate.

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1. Adebayo, I. A.; Arsad, H.; Gagman, H. A.; Ismail, N. Z.; Samian, M. R., 2020: Inhibitory Effect of Eco-Friendly Naturally Synthesized Silver Nanoparticles from the Leaf Extract of Medicinal Detarium microcarpum Plant on Pancreatic and Cervical Cancer Cells. Asian Pacific Journal of Cancer Prevention., 21, 1247–1252.
2. Agarwal, H.; Venkat Kumar, S.; Rajeshkumar, S., 2018: Antidiabetic effect of silver nanoparticles synthesized using lemongrass (Cymbopogon citratus) through conventional heating and microwave irradiation approach. Journal of Microbiology, Biotechnology and Food Sciences., 7, 371–376.
3. Ahmad, N.; Sharma, S.; Singh, V. N.; Shamsi, S. F.; Fatma, A.; Mehta, B. R., 2011: BiosynthesisofSilverNanoparticlesfromDesmodiumtriflorum ANovelApproachTowardsWeedUtilizatio.pdf. Biotechnology Research International., 2011, 1–8.
4. Ahmed, S.; Saifullah; Ahmad, M.; Swami, B. L.; Ikram, S., 2016: Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract . Journal of Radiation Research and Applied Sciences., 9, 1–7.
5. Anandalakshmi, K.; Venugobal, J.; Ramasamy, V., 2016: Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience., 6, 399–408.
6. Anandan, M.; Poorani, G.; Boomi, P.; Varunkumar, K.; Anand, K.; Chuturgoon, A. A.; Saravanan, M.; Prabu, H. G., 2019: Green synthesis of anisotropic silver nanoparticles from the aqueous leaf extract of Dodonaea viscosa with their antibacterial and anticancer activities. Process Biochemistry., 80, 80–88.
7. Bhakya, S.; Muthukrishnan, S.; Sukumaran, M.; Muthukumar, M., 2016: Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Applied Nanoscience., 6, 755–766.
8. Bora, K. S.; Sharma, A., 2011: The genus Artemisia: a comprehensive review. Pharmaceutical Biology., 49, 101–109.
9. Chand, K.; Abro, M. I.; Aftab, U.; Shah, A. H.; Lakhan, M. N.; Cao, D.; Mehdi, G.; Mohamed, A. M. A., 2019: Green synthesis characterization and antimicrobial activity against Staphylococcus aureus of silver nanoparticles using extracts of neem, onion and tomato. RSC advances., 9, 17002–17015.
10. Chand, K.; Cao, D.; Fouad, D. E.; Shah, A. H.; Dayo, A. Q.; Zhu, K.; Lakhan, M. N.; Mehdi, G.; Dong, S., 2020: Green synthesis, characterization and photocatalytic application of silver nanoparticles synthesized by various plant extracts. Arabian Journal of Chemistry.
11. Chen, M.; Yang, Z.; Wu, H.; Pan, X.; Xie, X.; Wu, C., 2011: Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel. International journal of nanomedicine., 6, 2873–2877.
12. Dai, H. Y.; Yang, H. M.; Jian, X.; Liu, X.; Liang, Z. H., 2017: Performance of Ag2O/Ag electrode as cathodic electron acceptor in microbial fuel cell. Acta Metallurgica Sinica (English Letters)., 30, 1243–1248.
13. Dauthal, P.; Mukhopadhyay, M., 2013: In-vitro free radical scavenging activity of biosynthesized gold and silver nanoparticles using Prunus armeniaca (apricot) fruit extract. Journal of nanoparticle research., 15, 1366.
14. Dzul-Erosa, M. S.; Cauich-Díaz, M. M.; Razo-Lazcano, T. A.; Avila-Rodriguez, M.; Reyes-Aguilera, J. A.; González-Muñoz, M. P., 2018: Aqueous leaf extracts of Cnidoscolus chayamansa (Mayan chaya) cultivated in Yucatán México. Part II: Uses for the phytomediated synthesis of silver nanoparticles. Materials Science and Engineering C., 91, 838–852.
15. Ebrahimzadeh, M. A.; Nabavi, S. M.; Nabavi, S. F.; Bahramian, F.; Bekhradnia, A. R.; others, 2010: Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci., 23, 29–34.
16. Gan, P. P.; Li, S. F. Y., 2012: Potential of plant as a biological factory to synthesize gold and silver nanoparticles and their applications. Reviews in Environmental Science and Bio/Technology., 11, 169–206.
17. Ghavami, S.; Hashemi, M.; Ande, S. R.; Yeganeh, B.; Xiao, W.; Eshraghi, M.; Bus, C. J.; Kadkhoda, K.; Wiechec, E.; Halayko, A. J.; Los, M., 2009: Apoptosis and cancer: Mutations within caspase genes. Journal of Medical Genetics., 46, 497–510.
18. Gilavand, F.; Saki, R.; Mirzaei, S. Z.; Esmaeil Lashgarian, H.; Karkhane, M.; Marzban, A., 2020: Green synthesis of zinc nanoparticles using aqueous extract of Magnoliae officinalis and assessment of its bioactivity potentials. Biointerface Research in Applied Chemistry.
19. Inbathamizh, L.; Ponnu, T. M.; Mary, E. J., 2013: In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. Journal of Pharmacy Research., 6, 32–38.
20. Jacob, S. J. P.; Finub, J. S.; Narayanan, A., 2012: Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line. Colloids and Surfaces B: Biointerfaces., 91, 212–214.
21. Jagtap, U. B.; Bapat, V. A., 2013: Green synthesis of silver nanoparticles using Artocarpus heterophyllus Lam. seed extract and its antibacterial activity. Industrial crops and products., 46, 132–137.
22. Keshari, A. K.; Srivastava, R.; Singh, P.; Yadav, V. B.; Nath, G., 2020: Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. Journal of Ayurveda and integrative medicine., 11, 37–44.
23. Markus, J.; Wang, D.; Kim, Y. J.; Ahn, S.; Mathiyalagan, R.; Wang, C.; Yang, D. C., 2017: Biosynthesis, characterization, and bioactivities evaluation of silver and gold nanoparticles mediated by the roots of Chinese herbal Angelica pubescens Maxim. Nanoscale research letters., 12, 46.
24. Moteriya, P.; Chanda, S., 2017: Synthesis and characterization of silver nanoparticles using Caesalpinia pulcherrima flower extract and assessment of their in vitro antimicrobial, antioxidant, cytotoxic, and genotoxic activities. Artificial cells, nanomedicine, and biotechnology., 45, 1556–1567.
25. Mousavi, B.; Tafvizi, F.; Zaker Bostanabad, S., 2018: Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artificial cells, nanomedicine, and biotechnology., 46, 499–510.
26. Nadagouda, M. N.; Speth, T. F.; Varma, R. S., 2011: Microwave-assisted green synthesis of silver nanostructures. Accounts of Chemical Research., 44, 469–478.
27. Patra, J. K.; Baek, K. H., 2016: Biosynthesis of silver nanoparticles using aqueous extract of silky hairs of corn and investigation of its antibacterial and anticandidal synergistic activity and antioxidant potential. IET Nanobiotechnology., 10, 326–333.
28. Poinern, G. E. J.; Chapman, P.; Le, X.; Fawcett, D., 2013: Green biosynthesis of gold nanometre scale plates using the leaf extracts from an indigenous Australian plant Eucalyptus macrocarpa. Gold Bulletin., 46, 165–173.
29. Rashid, M. I.; Mujawar, L. H.; Rehan, Z. A.; Qari, H.; Zeb, J.; Almeelbi, T.; Ismail, I. M. I., 2016: One-step synthesis of silver nanoparticles using Phoenix dactylifera leaves extract and their enhanced bactericidal activity. Journal of Molecular Liquids., 223, 1114–1122.
30. Ravichandran, V.; Vasanthi, S.; Shalini, S.; Shah, S. A. A.; Tripathy, M.; Paliwal, N., 2019: Green synthesis, characterization, antibacterial, antioxidant and photocatalytic activity of Parkia speciosa leaves extract mediated silver nanoparticles. Results in Physics., 15, 102565.
31. Ruttkay-Nedecky, B.; Krystofova, O.; Nejdl, L.; Adam, V., 2017: Nanoparticles based on essential metals and their phytotoxicity. Journal of Nanobiotechnology., 15, 33.
32. Saravanakumar, A.; Peng, M. M.; Ganesh, M.; Jayaprakash, J.; Mohankumar, M.; Jang, H. T., 2017: Low-cost and eco-friendly green synthesis of silver nanoparticles using Prunus japonica (Rosaceae) leaf extract and their antibacterial, antioxidant properties. Artificial cells, nanomedicine, and biotechnology., 45, 1165–1171.
33. Siddiqi, K. S.; Husen, A.; Rao, R. A. K., 2018: A review on biosynthesis of silver nanoparticles and their biocidal properties. Journal of nanobiotechnology., 16, 14.
34. Soman, S.; Ray, J. G., 2016: Silver nanoparticles synthesized using aqueous leaf extract of Ziziphus oenoplia (L.) Mill: characterization and assessment of antibacterial activity. Journal of Photochemistry and Photobiology B: Biology., 163, 391–402.
35. Tang, J.; Wang, C. K.; Pan, X.; Yan, H.; Zeng, G.; Xu, W.; He, W.; Daly, N. L.; Craik, D. J.; Tan, N., 2010: Isolation and characterization of cytotoxic cyclotides from Viola tricolor. Peptides., 31, 1434–1440.
36. Vilas, V.; Philip, D.; Mathew, J., 2016: Biosynthesis of Au and Au/Ag alloy nanoparticles using Coleus aromaticus essential oil and evaluation of their catalytic, antibacterial and antiradical activities. Journal of Molecular Liquids., 221, 179–189.
Journal of Agricultural Science and Technology
Volume 24, Issue 4
July and August 2022
Pages 885-900