Compositional Differences in Peel and Juice of Cracked and Normal Fruits of Lemon (Citrus limon (L.), Burm.)

Document Type : Original Research

Authors
R. Kaur 1
N. Kaur 1
H. Singh 2
M. Kaur Sangha 3
1 Department of Botany, Punjab Agricultural University, Ludhiana, 141004, India.
2 Department of Fruit Science, Punjab Agricultural University, Ludhiana, 141004, India.
3 Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India.
Abstract
Fruit cracking is a predominant physiological disorder of lemon that limits its productivity. The present study aimed to compare the physiological and biochemical traits of cracked and normal fruits of lemon, to understand the cause of fruit cracking and find a viable solution for this disorder. This study was conducted on five-year-old uniform healthy trees grown at fruit research farm, Punjab Agricultural University, Ludhiana, during 2017-2018. Fruits of lemon cracked in different patterns and the cracking peaked due to sudden rainfall and high humidity after a dry spell during the two consecutive years of study. The peel thickness, peel percent and chlorophyll content of the cracked peel was significantly low as compared to the normal ones. Activity of peroxidase and two cell wall degrading enzymes, namely, cellulase and polygalacturonase were higher in cracked peels. Juice content and ascorbic content were low in cracked fruit juice as compared to normal ones. Meanwhile, calcium, potassium and boron content were higher in the normal peel and lower in the cracked peel. A significant positive correlation of fruit cracking incidence with proline, peroxidase, cellulase and polygalacturonase was established, whereas a negative significant correlation was established between fruit cracking percent and peel thickness, calcium, potassium, boron, juice percent and ascorbic acid content. Nutrient deficiency and higher activities of cellulase and polygalacturonase in peel of cracked fruits emerged as the cause of fruit cracking incidence in lemon. Hence, foliar application of calcium, potassium, and boron are recommendable as a remedial measure for prevention of fruit cracking in lemon.

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A.O.A.C. 1990. Official methods of analysis. Association of analytical chemists, Washington DC, USA.
Ali, A., Summers, L. L., Klein, G. J. and Lovatt, C. J. 2000. Albedo breakdown in California. Proc. Int. Soc. Citric. 2: 1090-1093.
Alva, A. K., Mattos, J. D., Paramasivam, S., Patil, B. and Dou, H. 2006. Potassium management for optimizing citrus production and quality. Int. J. Fruit Sci. 6: 3-43.
Anonymous, 2018. Indian Horticulture database, NHB, Government of India.
Barry, G. H. and Bower, J. P. 1997. Manipulation of fruit set and stylar-end fruit split in ‘Nova’ mandarin hybrid. Sci. Hort. 70: 243-250.
Bates, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for stress studies. Plant Soil 39: 205-207.
Berger, K. C. and Truog, E. 1939. Boron determination in soils and plants. Ind Eng Chem Res 11: 540-545.
Brummell, D. A. 2006. Cell wall disassembly in ripening fruit. Func. Pl. Biol. 33: 103-119.
Chapman, H. D. and Pratt, P. F. 1961. Methods of Analysis for Soils, Plants and Waters. University of California, Los Angeles. P 60-61, 150-179.
Cronje, P. J. R., Barry G. H. and Huysamer, M. 2011. Fruit position during development of ‘Nules Clementine’ mandarin affects the concentration of K, Mg and Ca in the flavedo. Sci. Hort. 130: 829-837.
Garcia-Luis, A., Duarte, A. M. M., Kanduser, M. and Guardiola, J. L. 2001. The anatomy of fruit in relation to the propensity of citrus fruit to split. Sci. Hort. 87: 33-52.
Haq, I. and Rab, A. 2012. Foliar application of calcium chloride and borax affects the fruit skin strength and cracking incidence in litchi (Litchi chinensis Sonn.) cultivars. Afr. J. Biotechnol. 11: 2445-2453.
Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J. and Ahmad, A. 2012. Role of proline under changing environment: A review. Plant signaling behavior 7: 1456-1466.
Holtzhausen, L. C. 1981. Creasing: Formulating a hypothesis. Proc. Int. Soc. Citric. 1: 201-204.
Hutton, R. J., Landsberg, J. J. and Sutton, B. G. 2007. Timing irrigation to suit citrus phenology: a means of reducing water without compromising fruit yield and quality? Aust. J. Exp. Agric. 47: 71-80.
Kaur, R., Kaur, N. and Singh, H. 2019. Pericarp and pedicel anatomy in relation to fruit cracking in lemon (Citrus limon L Burm). Sci. Hort. 246: 462-68.
Khehra, S. and Bal, J. S. 2014. Influence of foliar sprays on fruit cracking in lemon. Int. J. Pl. Env. Sci. 4: 124-28.
Kramer, P. J. and Boyer, J. S. 1995. Water Relations of Plants and Soils. Academic Press, San Diego. p 495.
Li, J. 2009. Cell wall metabolism and unrelated gene isolation of pitting fruit peel in citrus. Ph.D. thesis, South China Agricultural University, Guangzhou, China.
Li, J. and Chen, J. 2017. Citrus fruit cracking: Causes and Occurrence. Horti. Pl. J. 3: 255-260.
Li, J., Zhang, P. P., Chen, J. Z., Yao, Q. and Jiang, Y. M. 2009. Cellular wall metabolism in citrus fruit pericarp and its relation to creasing fruit rate. Sci. Hort. 122: 45-50.
Lu, P. L. and Lin, C. H. 2011. Physiology of fruit cracking in wax apple (Syzygium samarangense). Botanica Orientalis: J. Pl. Sci. 8: 70-76.
Malik, C. P. and Singh, M. B. 1980. Plant Enzymology and Histo-enzymology: A Text Manual. Kalyani publishers, New Delhi. p 286.
Milad, R. E. and Shackel, K. A. 1992. Water relations of fruit and cracking in French prune (Prunus domestica L cv. French). J. Am. Soc. Hortic. Sci. 117: 824-828.
Morgan, K. T., Rouse R., E., Roka, F. M., Futich, S. H. and Zekri, M. 2005. Leaf and fruit mineral content and peel thickness of ‘Hamlin’ orange. Proc. Florida State for Horticultural Society 118: 19-21.
Pretel, M.T., Amoros, A., Botella, M.A., Serrano, M. and Romojaro, F. 2005. Study of albedo and carpelar membrane degradation for further application in enzymatic peeling of citrus fruits. J. Sci. Food Agri. 85: 86-90.
Price, J., Laxmi, A., St Martin, S. K. and Jang, J. C. 2004. Global transcription profiling reveals multiple sugar signal transduction mechanism in Arabidopsis. Plant Cell 16: 2128-2150.
Robinson, D.S. 1991. Peroxidases and their significance in Fruit and Vegetables. In: Food Enzymology. Fox PF (ed), Elsevier Science Publishers, London, p 399-426.
Saleem, B.A., Hassan, I., Singh, Z., Malik, A.M., Prevez, M.A. 2014. Comparative changes in the rheological properties and cell wall metabolism in rind of healthy and creased fruit of Washington Navel and Navelina sweet orange (Citrus sinensis) L. Aust. J. Crop Sci. 8: 62-70.
Sallato, B., Bonomellic, C. and Martiz, J. 2015. Differences in quality parameters and nutrients composition in Fukumoto oranges with and without creasing symptoms. J. Pl. Nutri. 40: 954-963.
Sandhu, S. and Bal, J. S. 2013. Quality improvement in lemon (Citrus limon L.) through integrated management of fruit cracking. Afr. J. Agric. Res. 8: 3552-3557.
Sdoodee, S. and Chiarawipa, R. 2005. Fruit splitting occurrence of Shogun mandarin (Citrus reticulata Blanco cv Shogun) in southern Thailand and alleviation by calcium and boron sprays. Songklanakarin J. Sci. Technol. 27: 719-30.
Thomas, R. L., Jen, J. J and Morr, C. V. 1981. Changes in soluble and bound peroxidase-IAA oxidase during tomato fruit development. J. Food Sci. 47: 158-161.
Tomas, B. F. A. and Espin, J. C. 2001. Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J. Sci. Food Agric. 81: 853-876.
Wang, N. and Qin, X. N. 1987. Effect of mineral nutrition levels on fruit splitting in Jin-Cheng orange. J. Southwest. Agric. Univ., 9: 458-461.
Journal of Agricultural Science and Technology
Volume 24, Issue 4
July and August 2022
Pages 861-872