Combined Application of Gamma-Aminobutyric Acid and Carnauba Wax as Edible Coating on Pomegranates in Cold Storage

Document Type : Original Research

Authors
F. Nazoori
E. ZamaniBahramabadi
A. Rafie
S. H. Mirdehghan
Department of Horticultural Sciences, College of Agriculture, Vali-e-Asr University of Rafsanjan, Kerman, Islamic Republic of Iran.
Abstract
Pomegranate is a popular fruit, rich in antioxidants and minerals but sensitive to postharvest storage. The efficiency of 5 and 10 mM γ-Aminobutyric Acid (GABA) combined with 0.5% carnauba wax as edible coating was investigated on extending the cold storage life of pomegranate fruit (Cv. Malas Saveh) after 45 and 90 days. Coatings maintained fruit freshness, inhibited the chilling injury symptoms, reduced peel malondialdehyde formation (minimum of 0.74 nM g-1), reduced loss of aroma/taste, and increased aril antioxidant activity (maximum of 94.9%). Aril anthocyanin content was more stable in 5 mM GABA coated than uncoated fruits. However, the coatings caused more weight loss on 45th day (11.0% in 10 mM GABA and 8.3% in the control). This parameter was similar in coated and uncoated fruits after 90 days. Aril phenolic content in coated fruits was higher on 90th day but not on 45th day (maximum of 0.08 mg.100g-1 in the control on day 45). Aril lightness increased in the control sample on 45th day while coated fruits were more similar to harvest time. After 90 days, the control and treated samples were similar. The color scales (a*, b*, and Chroma) values of peels and arils declined by storage without any significant effects of coatings. Peel and aril Hue did not change by storage time or coatings. Results suggested some benefits of coatings for retaining the postharvest quality of pomegranate fruits.

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1. Azizi, F., Erfani, M. J., Khademi, O. and Nourollahi, K. 2018. Effects of Oxalic Acid and Ascorbic Acid on Shelf Life of Pomegranate Arils. Iran. J. Food Sci. Technol., 14: 15–24 .
2. Barman, K., Asrey, R. and Pal, R. K. 2011. Putrescine and Carnauba Wax Pretreatments Alleviate Chilling Injury, Enhance Shelf Life and Preserve Pomegranate Fruit Quality During Cold Storage., Sci Hortic. 130: 795–800.
3. Chiumarelli, M. and Hubinger, M. D. 2014. Evaluation of Edible Films and Coatings Formulated with Cassava Starch, Glycerol, Carnauba Wax and Stearic Acid., Food Hydrocoll. 38: 20-27.
4. Dhall, R. K. 2013. Advances in Edible Coatings for Fresh Fruits and Vegetables: A Review. Crit. Rev. Food Sci. Nutr., 53(5): 435-450.
5. Dou, H. 2004. Effect of Coating Application on Chilling Injury of Grapefruit Cultivars. HortScience., 39(3): 558-561.
6. Ebrahimzadeh, A., Pirzad, F., Tahanian, H. and Soleimani Aghdam, M. 2019. Influence of Gum Arabic Enriched with GABA Coating on Oxidative Damage of Walnut Kernels. Food. Technol. Biotechnol., 57(4): 554-560.
7. Gao, H., Wu, S., Zeng, Q., Li, P. and Guan, W. 2018. Effects of Exogenous γ-Aminobutyric Acid Treatment on Browning and Foodborne Pathogens in Fresh-Cut Apples. Postharvest Biol Technol., 146: 1-8.
8. Germano, T. A., Aguiar, R. P., Bastos, M. S. R., Moreira, R. A., Ayala-Zavala, J. F. and de Miranda, M. R. A. 2019. Galactomannan-Carnauba Wax Coating Improves the Antioxidant Status and Reduces Chilling Injury of ‘Paluma’ guava. Postharvest Biol Technol., 149: 9-17.
9. Gohari Ardabili, A., Farhoosh, R. and Haddad Khodaparast, M. H. 2011. Chemical Composition and Physicochemical Properties of Pumpkin Seeds (Cucurbita pepo Subsp. pepo Var. Styriaka) Grown in Iran. JAST., 13(7): 1053-1063.
10. Gonçalves, F. P., Martins, M. C., Silva Junior, G. J., Lourenco, S. A. and Amorim, L. 2010. Postharvest Control of Brown Rot and Rhizopus Rot in Plums and Nectarines Using Carnauba Wax. Postharvest Biol Technol., 58(3): 211-217.
11. Habibi, F., Ramezanian, A., Rahemi, M., Eshghi, S., Guillen, F., Serrano, M. and Valero, D. 2019. Postharvest Treatments with γ‐aminobutyric Acid, Methyl Jasmonate, or Methyl Salicylate Enhance Chilling Tolerance of Blood Orange Fruit at Prolonged Cold Storage. J Sci Food Agric., 99(14): 6408-6417.
12. Horzum, Ö., Akan, S. and Tuna Güneş, N. 2019. Pomegranate: Red Health Agent. International Eurasian Congress on Natural Nutrition, Healthy Life and Sport 2019, Ankara, 1507-1514.
13. Kashash, Y., Mayuoni-Kirshenbaum, L., Goldenberg, L. and Choi, H. J. 2016. Effects of Harvest Date and Low-Temperature Conditioning on Chilling Tolerance of ‘Wonderful’ Pomegranate Fruit. Sci. Hortic., 209: 286-292.
14. Kellerman, M., Erasmus, A., Cronje, P. J. R. and Fourie, P. H. 2014. Thiabendazole Residue Loading in Dip, Drench and Wax Coating Applications to Control Green Mould and Chilling Injury on Citrus Fruit. Postharvest Biol Technol., 96: 78-87
15. Kumar, N., Pratibha, Neeraj, Ojha, A., Upadhyay, A., Singh, R. and Kumar, S. 2020. Effect of Active Chitosan-Pullulan Composite Edible Coating Enrich with Pomegranate Peel Extract on the Storage Quality of Green Bell Pepper, LWT - Food Sci Technol., https://doi.org/10.1016/j.lwt.2020.110435.
16. Kumar, N. and Neeraj. 2019. Polysaccharide-Based Component and their Relevance in Edible Film/Coating: A Review. Nutr. Food Sci., 49(5): 793-823.
17. McGuire, R. G. 1997. Quarantine Treatment and Application of Carnauba Wax Coating. HortScience., 32(2): 271-274
18. McGuire, R. G. and Hall, G. J. 1995. Coating Guavas with Cellulose- or Carnauba-Based Emulsions Interferes with Postharvest Ripening. HortScience., 30(2): 294–295.
19. Meighani, H., Ghasemnezhad, M. and Bakhshi, D. 2014. Effect of Different Coatings on Post-Harvest Quality and Bioactive Compounds of Pomegranate (Punica granatum L.) Fruits. J. food sci. technol., 52(7): 4507-4514.
20. Mirdehghan, S. H. and Rahemi, M. 2005. Effects of Hot Water Treatment on Reducing Chilling Injury of Pomegranate (Punica granatum) Fruit during Storage. Acta Hort., 682: 887-892.
21. Moradinezhad, F., Ansarifar, E. and Mohammadian Moghaddam, M. 2020. Extending the Shelf Life and Maintaining Quality of Minimally-Processed Pomegranate Arils Using Ascorbic Acid Coating and Modified Atmosphere Packaging. J. Food Meas. Charact., 14: 3445–3454.
22. Orthofer, R. and Lamuela-Raventos, R. 1999. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin–Ciocalteu, Reagent. Method Enzymol., 299: 152–178.
23. Purvis, A. C. 1994. Interaction of Waxes and Temperature in Retarding Moisture Loss from and Chilling Injury of Cucumber Fruit During Storage. Proc. Fla. State Hor Soc., 107: 257–260.
24. Rapisarda, P., Fanella, F. and Maccarone, F. 2000. Reliability of Analytical Methods for Determining Anthocyanin in Blood Orange Juices. J. Agri. Food Chem., 48: 2249–2252.
25. Rastegar, S., Khankahdani, H. H. and Rahimzadeh, M. 2020. Effect of γ-Aminobutyric Acid on the Antioxidant System and Biochemical Changes of Mango Fruit During Storage. Food Measure., 14: 778-789.
26. Sayyari, M., Soleimani Aghdam, M., Salehi, F. and Ghanbari, F. 2016. Salicyloyl Chitosan Alleviates Chilling Injury and Maintains Antioxidant Capacity of Pomegranate Fruits During Cold Storage. Sci Hortic., 211: 110-117.
27. Shang, H., Cao, S., Yang, Z., Cai, Y. and Zheng, Y. 2011. Effect of Exogenous Gamma Aminobutyric Acid Treatment on Proline Accumulation and Chilling Injury in Peach Fruit After Long-term Cold Storage. J Agri Food Chem., 59: 1264-1268.
28. Shekari, A., Naghshiband Hassani, R. and Soleimani Aghdam, M. 2021. Exogenous Application of GABA Retards Cap Browning in Agaricus bisporus and its Possible Mechanism. Postharvest Biol Technol., 174: 111434.
29. Singh, S., Khemariya, P., Rai, A., Rai, A. C., Koley, T. K. and Singh, B. 2016. Carnauba Wax-based Edible Coating Enhances Shelf-Life and Retain Quality of Eggplant (Solanum melongena) Fruits. LWT., 74: 420-426.
30. Soleimani Aghdam, M., Razavi, F. and Karamneghad, F. 2015. Maintaining the Postharvest Nutritional Quality of Peach Fruits by γ-Aminobutyric Acid. Iran. J. Plant Physiol., 5(4): 1457–1463.
31. Valenzuela, J. L., Manzano, S., Palma, F., Carvajal, F., Garrido, D. and Jamilena, M. 2017. Oxidative Stress Associated with Chilling Injury in Immature Fruit: Postharvest Technological and Biotechnological Solutions. Int. J. Mol. Sci., 18: 1467.
32. Varasteh, F., Arzani, K., Barzegar, M. and Zamani, Z. 2012. Changes in Anthocyanins in Arils of Chitosan-coated Pomegranate (Punica granatum L. cv. Rabbab-e-Neyriz) Fruit During Cold Storage. Food Chem., 130: 267–272.
33. Wang, L., Chena, S., Kong, W., Li, S. and Archbold, D. D. 2006. Salicylic Acid Pretreatment Alleviates Chilling Injury and Affects the Antioxidant System and Heat Shock Proteins of Peaches During Cold Storage. Postharvest Biol Technol., 41: 244-251.
34. Wang, L., Zhang, H., Jin, P., Guo, X., Li, Y., Fan, C., Wang, J. and Zheng, Y. 2016. Enhancement of Storage Quality and Antioxidant Capacity of Harvested Sweet Cherry Fruit by Immersion with β-Aminobutyric Acid. Postharvest Biol Technol., 118: 71–78.
35. Wang, Y., Luo, Z. and Huang, H. 2014. Effect of Exogenouse γ-Aminobutyric Acid (GABA) Treatment on Chilling Injury and Antioxidant Capacity in Banana Peel. Sci Hortic., 168: 132–137.
36. Yildiz, M., Varis, B., Horzum, O. and Tuna Gunes, N. 2020. Postharvest Salicylic Acid Treatment Influences Some Quality Attributes in Air-Stored Pomegranate Fruit. J. Agri. Sci. Tarım Bilim. Dergi. DOI: 10.15832/ankutbd.549669
37. Zarbakhsh, S., Kazemzadeh-Beneh, H. and Rastegar, S. 2019. Quality Preservation of Minimally Processed Pomegranate cv. Jahrom Arils Based on Chitosan and Organic Acid Edible Coatings. J food safe., 40(2): 1-12.
Journal of Agricultural Science and Technology
Volume 24, Issue 3
May and June 2022
Pages 591-602