Metabolic and Enzymatic Responses of Calendula officinalis L. to Foliar Application of Spermidine, Citric Acid and Proline under Drought Stress in a Post Harvest Condition

Document Type : Original Research

Authors
1 Department of Horticultural Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad katoul, Islamic Republic of Iran.
2 Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Islamic Republic of Iran.
3 Department of Horticulture Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Islamic Republic of Iran.
Abstract
The first experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (0, 50 and 100 mg L-1) on some metabolites and enzymatic activities of Calendula officinalis L. under drought stress (No stress: Control, 25, 50, and 75% field capacity). The second experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (0, 50 and 100 mg L-1) on the post-harvest shelf life of flowers (beginning of the experiment, 5, and 10 days post-harvest). The experiments were factorial in a completely randomized design with 3 replications. All treatments had a significant effect on the measured variables. In the first experiment, FC 25% reduced all traits, FC 75% increased carotenoid, carbohydrate, phenol, flavonoid, protein, Peroxidase (POD) and Superoxide Dismutase (SOD) activities. The highest vitamin C was observed in the control FC. Also, foliar application of 100 mg L-1 proline, increased carotenoid, carbohydrate and phenol, 50 mg L-1 proline increased protein content. Also, 100 mg L-1 of spermidine increased flavonoid and 100 mg L-1 of citric acid increased vitamin C, SOD and POD activities. In the second experiment, all evaluated traits were reduced ten days after harvest, the highest post-harvest life was for 100 mg L-1 of citric acid (9.7 days) and the lowest was for the control (5.3 days). The study results showed that application of 100 mg L-1 spermidine, citric acid and proline with FC 75% improved biochemical properties, nutritional traits, and post-harvest life of Pot marigold.

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1. Abd Elbar, O., Farag, R. and Shehata, S. 2019. Effect of putrescine application on some growth, biochemical and anatomical characteristics of Thymus vulgaris L. under drought stress. Ann. Agric. Sci., 64(2): 129-137.
2. Abd-Elhamid, E.M., Sadak, M.S. and Tawfik, M.M. 2016. Physiological response of Fenugreek plant to the application of proline under different water regimes. Pharm. Biol. Chem. Sci., 7(3): 580-594.
3. Acemi, A., Avcı Duman, Y., Yuzugullu Karakus, Y. and Özen, F. 2018. Developmental and biochemical analyses of in vitro drought stress response in ornamental European Bluestar (Amsonia orientalis Decne.). Folia Hort., 30(2): 357-366.
4. Ali, Q., Anwar, F., Ashraf, M., Saari, N. and Perveen, R. 2013. Ameliorating Effects of Exogenously Applied Proline on Seed Composition, Seed Oil Quality and Oil Antioxidant Activity of maize (Zea mays L.) under Drought Stress. Int J Mol Sci., 14(1): 818-835.
5. Ali, RM., Abbas, H.M. and Kamal, R.K. 2007. The effect of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram. Plant Soil Environ, 53(12): 529-543.
6. Aliyari, P., Bakhshi Kazaj, F., Barzegar, M. and Ahmadi Gavlighi, H. 2020. Production of functional sausage using pomegranate peel and pistachio green hull extracts as natural preservatives. J. Agr. Sci. Tech., 22(1): 159-172.
7. Amirjani, MR., Abnosi, MH., Mahdiyeh, M., Ghareh shaikhloo, S. 2015. Investigation the Effect of Lead on the Activity of Antioxidant Enzymes, Level of Prolin and Alkaloid in the Callus of Catharantus roseus. Cell Tissue Res. 6(1): 9-21.
8. Ashraf, M., Akram, N.A., Al-Qurainy, F. and Flooda, M.R. 2011. Drought tolerance: roles of organic osmolyts, growth, regulators, and mineral nutrients. Adv. Agron., 111: 249-296.
9. Baniasadi, F., Saffari, V.R. and Maghsoudi Moud, A.A. 2018. Physiological and growth responses of Calendula officinalis L. plants to the interaction effects of polyamines and salt stress. Sci Hortic., 234: 312–317.
10. Cavasini, R., Laschi, D., Tavares, A. and Lima, G. 2018. Carbohydrate reserves on postharvest of lisianthus cut flowers. J. Ornam. Hortic., 24(1):12-18.
11. Chang, C.C., Yang, M.H., Wen H.M. and Chern J.C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal., 10(3): 178-182.
12. Da Silva, J.A.T. 2003.The cutflower: postharvest considerations. Res. J. Biol. Sci., 3(4): 406–442.
13. Du Preez, D.R. and Bale, G.C. 2008. A simple method for the quantitative recovery of nitrate–N during the Kjeldahl analysis of dry soil and plant samples. Commun. Soil. Sci. Plant Anal. 345-357.
14. Eidyan, B., Hadavi, E. and Moalemi, N. 2014. Pre-harvest foliar application of iron sulfate and citric acid combined with urea fertigation affects growth and vase life of tuberose (Polianthes tuberosa L.) ‘Por-Par’. Hort. Environ. Biotechnol., 55(1): 9–13.
15. Ezhilmathi, K., Singh, V., Arora, P. and Sairam, R.K. 2007. Effect of 5-sulfocalicylic acid on antioxidant in relation to vase life of gladiolus cut flower. Plant Growth Regul., 51: 99-108.
16. Farahbakhsh, H. and Pasandipour, A. 2017. Physiological response of Henna, medicinal-industrial plant, to application of Salicylic Acid under drought Stress. j.plant proc. func., 6(19): 233-246.
17. Farhoudi, R., Lee, D.J. and Hussain, M. 2014. Mild drought improves growth and flower oil productivity of German Chamomile (Matricaria recutita L.). J Essent Oil Bear Pl., 17(1): 26-31.
18. Gharibi, SH., Tabatabaei, B.E., Saeidi, Gh. and Goli, S.A. 2016. Effect of drought stress on total phenolic, lipid peroxidation, and antioxidant activity of Achillea species. Biotechnol. Appl. Biochem., 178(4):796-809.
19. Gholami Zali, A. and Ehsanzadeh, P. 2018. Exogenous proline improves osmoregulation, physiological functions, essential oil, and seed yield of fennel. Ind Crops Prod., 111:133–140.
20. Habibi, Gh. 2018. Effects of mild and severe drought stress on the biomass, phenolic compounds production and photochemical activity of Aloe vera (L.) Burm.f. Acta Agric. Slov., 111(2): 463 - 476.
21. Idrees, M., Khan, M.M.A., Aftab, T., Naeem, M. and Hashmi, N. 2010. Salicylic acidinduced physiological and biochemical changes in lemongrass varieties under water stress. J Plant Interact., 5(4): 293-303.
22. Jalili Marandi, R. 2012. Postharvest physiology. West Azerbaijan University Jihad Publications. 150-153.
23. Jin, J., Lauricella, D., Armstrong, R., Sale, P. and Tang, C. 2015. Phosphorus application and elevated CO2 enhance drought tolerance in field pea grown in a phosphorus-deficient vertisol. Ann Bot., 116(6): 975–985.
24. Jubany-Marí, T., Munné-Bosch, S., Alegre, L. 2010. Redox regulation of water stress responses in field-grown plants. Role of hydrogen peroxide and ascorbate. Plant Physiol Biochem., 48(5): 351-358.
25. Kelley, K. M., Cameron, A. C., Biernbaum, J. A. and Poff, K. L. 2003. Effect of storage temperature on the quality of edible flowers. Postharvest Biol. Technol, 27(3): 341-344.
26. Khalil Soha, E. and El-Noemani, A.A. 2012. Effect of irrigation intervals and exogenous proline application in improving tolerance of garden cress plant (Lepidium sativum L.) to water stress. Res. J. Appl. Sci., 8(1): 157-167.
27. Kou, L., Turner, E. R. and Luo, Y. 2012. Extending the shelf life of edible flowers with controlled release of 1‐methylcyclopropene and modified atmosphere packaging. J. Food Sci, 77(5): S188-S193.
28. Kozminska, A., Al Hassan, M., Kumar, D., Oprica, L., Martinelli, F., Grigore, M.N., Vicente, O., Boscaiu, M. 2017. Characterizing the effects of salt stress in Calendula officinalis L. J.Appl. Bot. Food Qual., 90: 323-329.
29. Leonti, M. 2012. The co-evolutionary perspective of the food-medicine continuum and wild gathered and cultivated vegetables. Genet Resour Crop Ev., 59(7): 1295-1302.
30. Miri, M.S., Ahmadi, S. and Moradi, P. 2015. Influence of salicylic acid and citric acid on the growth, biochemical characteristics and essential oil content of Thyme (Thymus vulgaris L.). J. Med. Plants Res., 2: 141-146.
31. Mohammadi, H., Imani, A., Abdosi, V., Asghari, M. R. and Talaei, A. R. 2020. Exogenous salicylic acid mitigates adverse effects of salinity on some photosynthesis-related parameters of Almond. J. Agr. Sci. Tech., 22(2): 519-534.
32. Mujahid, F., Shafaqat, A., Rizwan, M., Ali, Q. and HussainBukhari, A. 2017. Citric acid assisted phytoextraction of chromium by sunflower; morpho physiological and biochemical alterations in plants. Ecotoxicol. Environ. Saf., 145: 90-102.
33. Naeemi, M., Ali Akbari, Gh., Shirani Rad, A.H., Hassanloo, T. and Abbas Akbari, Gh. 2012. Effect of zeolite application and selenium spraying on water relations traits and antioxidant enzymes in medicinal pumpkin (Cucurbita pepo L.) under water deficit stress conditions. J. Crop Improv., 14(1): 67-81.
34. Naghizadeh, M., Kabiri, R., Hatami, A., Oloumi, H., Nasibi, F. and Thahmasei, Z. 2019. Exogenous application of melatonin mitigates the adverse effects of drought stress on morpho-physiological traits and secondary metabolites in Moldavian balm (Dracocephalum moldavica). Physiol Mol Biol Plants., 25(4): 881-894.
35. Ohe, M., Rapolu, M., Mieda, T., Miyagawa, Y., Yabuta, Y., Yoshimura, K. and Shigeoka, S. 2005. Decline in leaf photooxidative-stress tolerance with age in tobacco. Plant Sci. J., 168(6): 1487-1493.
36. Pal, M., Szalai, G. and Janda, T. 2015. Speculation: Polyamines are important in abiotic stress signaling. Plant Sci. J., 237: 16-23.
37. Pandey, H.C., Baig, M.J., Chandra, A. and Bhatt, R.K. 2010. Drought stress induced changes in lipid peroxidation and antioxidant system in genus Avena. J Environ Biol., 31(4): 435-440.
38. Parvin, S., Lee, OR., Sathiyaraj, G., Khorolragchaa, A., Kim, YJ. and Yang, DC. 2014. Spermidine alleviates the growth of saline-stressed ginseng seedlings through antioxidative defense system. Gene., 537(1): 70-78.
39. Redha, A., Al-Mansor, N., Suleman, P., Hasan. RA. and Afzal, M. 2012. Modulation of antioxidant defenses in Conocarpus lancifolius under variable abiotic stress. Biochem Syst Ecol., 43: 80–86.
40. Remorini, D., Tavarini, S., Degl, E., Loreti F., Massai R. and Guidi, L. 2008. Effect of root stock and harvesting time on the nutritional quality of peel and flesh of peach fruits. Food Chem., 110(2): 361-367.
41. Roosta, H.R., Mohammadian, F., Raghami, M., Hamidpour, M. and Mirdehghan, S. H. 2020. Effect of nutrient solution and pruning on plant growth, yield, and fruit quality of hot pepper grown in an NFT system. J. Agr. Sci. Tech., 22(6): 1-14.
42. Ruoyi, K., Zhifang, Y. and Zhaoxin, L. 2005. Effect of coating and intermittent warming on enzymes, soluble pectin substances and ascorbic acid of Prunus persica (Cv. Zhonghuashoutao) during refrigerated storage. Food Res. Int., 38(3): 331-336.
43. Sarvandi, S., Ehtesham Nia, A., Rezaei Nejad, A. and Azimi, M.H. 2020. Morpho-Physiological responses of some Iris cultivars under drought and salinity stresses. J. Agr. Sci. Tech., 22(2): 535-546.
44. Sedghi, S., Seyed Sharifi, R., Pirzad, A.R. and Amanpour-Balaneji, B. 2012. Phytohormonal regulation of antioxidant systems in petals of drought stressed pot marigold (Calendula officinalis L.). .J. Agr. Sci. Tech., 14(4): 869-878.
45. Seyoum, A., Asres, K. and El-Fiky, F.K. 2006. Structure radical scavenging activity relationships of flavonoid. Phytochemistry., 67(18): 2058-2070.
46. Shams Najafabadi, N., Sahari1, M. A., Barzegar, M. and Hamidi Esfahani, Z. 2020. Role of extraction conditions in the recovery of some phytochemical compounds of the Jujube fruit. J. Agr. Sci. Tech., 22(2): 439-451.
47. Sharma, S.P., Leskovar D.I., Crosby K.M., Volder, A. and Ibrahim, A.M.H. 2014. Root growth, yield, and fruit quality responses of reticulatus and inodorus melons (Cucumis melo L.) to deficit subsurface drip irrigation. Agric. Water Manag., 136: 75–85.
48. Shetty, K. and Wahlqvist, ML. 2004. A model for the role of the proline-linked pentose-phosphate pathway in phenolic phytochemical bio-synthesis and mechanism of action for human health and environmental applications. Asia Pac J Clin Nutr., 13(1): 1-24.
49. SodaieZadeh, H., Shamsayi, M., Tajamolian M., Mirmohammadi Meybodi, A. and Hakimzadeh, M. 2016. The effect of drought stress on some morphological and physiological traits of Satureja hortensis. j.plant proc. func., 5(15): 1-11.
50. Song, W.Y., Zhang, Z.B., Shao, H.B., Guo, X.L., Cao, H.X., Zhao, H.B., Fu, Z.Y. and Hu, X.J. 2008. Relationship between calcium decoding elements and plant abiotic-stress resistance. Int J Biol Sci., 4(2): 116-125.
51. Sood, Sh. and Nagar, PK. 2008. Post-harvest alterations in polyamines and ethylene in two diverse rose species. Acta Physiol. Plant., 30(2): 243-248.
52. Spinardi, A.M. 2005. Effect of harvest date and storage on antioxidant systems in pears. Acta Hortic., 682: 655-662.
53. Szabados, L. and Savoure, A. 2009. Proline: a multifunctional amino acid. Trends in Plant Sci., 15: 89-97.
54. Taiz, L. and Zeiger, E. 2006. Plant Physiology. Forth Edition. Sinauer Associates, Inc., Publishers Sunderland, Massachusetts.
55. Tajvar, Y., Fotouhi Ghazvini, R., Hamidoghli, Y. and Hassan Sajedi, R. 2011. Antioxidant changes of Thomson navel orange (Citrus sinensis) on three rootstocks under low temperature stress. Hortic Environ Biotechnol., 52(6): 576-580.
56. Tian, Z., Wang, F., Zhang, W., Liu, CH. and Zhao, X. 2012. Antioxidant mechanism and lipid peroxidation patterns in leaves and petals of marigold in response to drought stress. Hortic Environ Biotechnol, 53(3): 183–192.
57. Toberman, H., Evans, C., Freeman, C., Fenner, N., White, M. and Artz, R.R. 2008. Summer drought effects upon soil and litter extracellular phenol oxidase activity and soluble carbon release in an upland Calluna heathland. Soil Biol. Biochem., 40(6): 1519-1532.
58. Valizadeh Ghale Beig, A., Nemati, H., Tehranifar, A., Emami, H. 2015. The effect of superabsorbent A 200, bentonite and drought stress on physiological traits and vitamin C of lettuce in greenhouse cultivation conditions. J. Sci. & Technol. Greenhouse Culture, 6(21):157-167.
59. Vendruscolo, E.C., Schuster, I., Pileggi, M., Scapim, C.A., Molinari, H.B., Marur, C.J. and Vieira, L.G. 2007. Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. J. Plant Physiol., 164(10): 1367-1376.
60. Yiu, JC., Juang, LD., Fang, DYT., Liu, CW. and Wu, SJ. 2009. Exogenous putrescine reduces flooding-induced oxidative damage by increasing the antioxidant properties of Welsh onion. Sci Hortic., 120(3): 306-314.
61. Yoruk, I.H., Demir, H., Ekici, K. and Sarvan, A. 2005. Purification and properties of catalase from Van Apple (Golden Delicious). Pakistan J Nutr., 4(1): 8-10.