Growth, Physiological and Metabolic Responses of Gerbera (Gerbera jamesonii L.) to Various Combinations of Calcium and Humic Acid Levels

Document Type : Original Research

Authors
M. Haghighi
A. Nikbakht
Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, Islamic Republic of Iran.
Abstract
Gerbera is one of the significant cut flower crops worldwide suffering from loss of flower quality induced mainly by Calcium (Ca) deficiency. In this research, the influence of Humic Acid (HA) and Ca in nutrient solution was studied on the growth parameters of gerbera. A completely randomized hydroponic experiment was designed by adding HA (0, 100, 500, and 1,000 mg L-1) and Ca (3.5 and 7 meq L-1 nutrient solution) to the nutrient solution of gerbera, with three replications. The effects of the treatments were evaluated on the growth, protein content, proline content, transpiration, CO2 assimilation, photosynthesis, SPAD value, number of harvested flower, and antioxidant activity in gerbera cv. Malibu. Results showed that decreasing Ca level to 3.5 meq L-1 decreased Superoxide Dismutase (SOD), Peroxidase (POD), and CO2 assimilation. However, this treatment caused an increase in Malondialdehyde (MDA), protein content, proline content, chlorophyll, and photosynthesis. Transpiration and number of harvested flowers were not affected by Ca concentration significantly. The highest level of HA (1,000 mg L-1) increased POD and transpiration (30 and 11%, respectively). However, SOD and protein content increased at 500 and 1,000 mg L-1 HA levels. When HA was accompanied with Ca, SPAD value, transpiration, and CO2 assimilation were improved, especially at high levels of HA (500 and 1,000 mg L-1) and higher level of Ca (7 meq L-1 Ca). The results suggested that HA could increase the number of harvested flowers and improve plant health by enhancing the plant enzymatic antioxidant defense system.

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1. Albino-Garduño, R., Zavaleta-Mancera, H. A., Ruiz-Posadas, L. M., Sandoval-Villa, M. and Castillo-Morales. 2008. Response of Gerbera to Calcium in Hydroponics. J. Plant Nutr., 31: 91-101.
2. Atkinson, C. J., Ruiz, L. P. and Mansfield, T. A. 1992. Calcium in the Xylem Sap and the Regulation of its Delivery to the Shoot. J. Exp. Bot., 43: 1315-1324.
3. Beauchamp, C. and Fridovich, I. 1971. Superoxide Dismutase; Improved Assays and an Assay Applicable to Acrylamide Gels. Anal. Biochem., 44: 276-287.
4. Bradford, M. M. 1976. A Rapid and Sensitive Method for Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem., 72: 248-254.
5. Carafoli, E. and Klee, C. B. 1999. Calcium as a Cellular Regulator. Oxford University Press, New York, 656 p.
6. Chandlee, J. M. and Scandalios, J.C. 1984. Analysis of Variants Affecting the Catalase Development Program in Maize Scutellum. Theor. Appl. Genet., 69: 71-77.
7. Chenm, Y. and Aviad, T. 1990. Effects of Humic Substances on Plant Growth. In: Humic Substances in Soil and Crop Sciences: Selected Readings: Am. Soc. Agr. Soil Sci. Mad., Wisconsin, pp. 161-186.
8. Chen, Y., Clapp, C. E., Magen, H. and Cline, V. W. 1999. Stimulation of Plant Growth by Humic Substances: Effects on Iron Availability. In: (Eds.) Ghabbour, E. A. and Davies, G. Understanding Humic Substances: R. Soc. Chem., Cambridge, pp. 1-17.
9. Cheng, H., Xu, W., Liu, J., Zhao, Q., He, Y. and Chen, G. 2007. Application of Composted Sewage Sludge (CSS) as a Soil Amendment for Turfgrass Growth. Ecol. Eng., 29: 96–104.
10. D'Agliano, G., Carrai, C. and Bigongiari, G .1994. Preliminary Evaluation of a Hydroponic Recirculating Nutrient System for Gerbera Cultivation. Acta. Hortic., 361: 414-422.
11. Dhindsa, R. S., Plumb-Dhindsa, P. and Thorpe. T. A. 1981. Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase. J. Exp. Bot., 32: 93-101.
12. Ferrara, G., Pacifico, A., Simeone, P. and Ferrara, E. 2007. Preliminary Study on the Effects of Foliar Applications of Humic Acids on "Italia" Table Grape. International Proceedings of Xxxth OIV World Congress of Vine and Wine Budapest.
13. Haghighi, M., Nikbakht, A., Xia, Y. P. and Pessarakli, M. 2013. Effects of humic acid addition to nutrient solution on growth, nutrient efficiency and postharvest attributes of gerbera. Commun. Soil Sci. Plant Anal. (In press).
14. Hyacinthe LG.,Florian P., Jean-Marc D., Françoise Gillet, Jérôme Pelloux, and Catherine Rayon.2015.Cell wall metabolism in response to abiotic stress. Plants (Basel). 4(1): 112–166.
15. Haghighi, M. 2012. The Effect of Humic and Glutamic Acids in Nutrient Solution on the N Metabolism in Lettuce. J. Sci. Food Agric., 92(15): 3023-3028.
16. Haghighi, M., Kafi, M., Fang, P. and Gui-Xiao, L. 2010. Humic acid decreased hazardous of cadmium toxicity on lettuce (Lactuca sativa L). Veg. Crops Res. Bull., 72: 49-61.
17. Issa, M., Ouzounidou, G., Maloupa, H., Isis, H. A. and Constantinidou, A. 2001. Seasonal and diurnal photosyntetic responses of two gerbera cultivars to different substrates and heating systems. Hortic. Sci., 88: 215-234.
18. Keiji J., Luciano P. C., Alfonso A., Lidiane Figueiredo S. , Rafael L.F.R., Daiane C. Baia , Natália O.A. C., Travis L. G. and Fábio Lopes O. 2020. Interaction between humic substances and plant hormones for phosphorous acquisition .Agronomy, 10(5): 640 https://doi.org/10.3390/agronomy10050640
19. Liu, C., Cooper, R. J. and Bowman, D. C. 1998. Humic Acid Application Affects Photosynthesis, Root Development, and Nutrient Content of Creeping Bentgrass. Hortic. Sci., 33(6): 1023-1025.Lobartini, J. C., Orioli, G. A. and Tan, K. H. 1997. Characteristics of soil humic acid fractions separated by ultrafiltration. Commun. Soil Sci. Plant Anal., 28: 787-796.
20. Mansfield, T. A., Hetherington, A. M. and Atkinson, C. J. 1990. Some current aspects of stomatal physiology. Annu. Rev. Plant Biol., 41: 55-75.
21. Mencarelli, F., Agostini, R., Botondi, R. and Massantini, R. 1995. Ethylen Production, ACC Content, PAL and POD Activity in Excised Sections of Straight and Bent Gerbera Scapes. J. Hortic. Sci., 70: 409-416.
22. Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. 2002. Physiological Effects of Humic Substances on Higher Plants. Soil Biol. Biochem., 34: 1527-1536.
23. Nikbakht, A., Kafi, M., Babalar, M., Xia, Y. P., Luo, A. and Etemadi, N. 2008. Effect of Humic Acid on Plant Growth, Nutrient Uptake, and Postharvest Life of Gerbera. J. Plant Nutr., 31(12): 2155-2167.
24. Panuccio, M. R., Muscolo, A. and Nardi, S. 2001. Effect of humic substances on nitrogen uptake and assimilation in two species of Pinus. J. Plant Nutr., 24: 693-704.
25. Roberts, D. M. and Harmon, A. C. 1992. Calcium modulated proteins: targets of intracellular calcium signals in higher plants. Annu. Rev. Plant Biol., 43: 375-414.
26. Ruiz, L. P., Atkinson, J. C. and Mansfield, T. A. 1992. Calcium in the Xylem and Its Influence on the behavior of stomata. J. Exp. Bot., 43: 1315-1324.
27. Salisbury, B. R. and Ross, C. W. 1994. Fisiolog' ıa Vegetal [Plant Physiology]. Grupo Editorial Iberoamericana, M'exico, pp. 138-141.
28. Savvas, D. and Gizas, G. 2002. Response of Hydroponically Grown Gerbera to Nutrient Solution Recycling and Different Nutrient Cation Ratios. Sci. Hortic., 96: 267-280.
29. Sujatha, K., Gowda, J V N. and Khan, M M. 2002. Effects of different fertigation levels on gerbera under low cost greenhouse. J. Orn. Hort. 5(1): 54-59.
30. Tejada, M. and Gonzalez, J. L. 2003. Influence of foliar fertilization with amino acids and humic acids on productivity and quality of asparagus. Biol. Agric. Hortic., 21: 277-291.
31. Varanini, Z. and Pinton, R. 2001. Direct Versus Indirect Effects of Soil Humic Substances on Plant Growth and Nutrition. In: (Eds.) Pinton, R., Varanini, Z. and Nannipieri, P. The Rhizosphere: Biochemistry and Organic Substances at the Soil Plant Interface. Marcel Dekker, New York, pp. 141- 158
32. Yang, C. M., Wang, M. C., Lu, Y. F., Chang, I. F. and Chou, CH. 2004. humic substances affect the activity of chlorophyllase. J. Chem. Ecol., 30(5): 1057-1065.
Journal of Agricultural Science and Technology
Volume 23, Issue 5
September and October 2021
Pages 1119-1129