Cold Tolerance in Olive Leaves of Three Cultivars Related to Some Physiological Parameters during Cold Acclimation and De-Acclimation Stages

Document Type : Original Research

Authors
Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, 84156–83111, Islamic Republic of Iran.
Abstract
This research studied changes in antioxidant enzymes activity, Total Soluble Proteins (TSPs), Malondialdehyde (MDA), and proline content in the leaves of three olive (Olea europaea L.) cultivars (Amphisis, Gorgan, and Manzanilla) at five different dates, and investigated their relationship with cold tolerance. The results revealed that cold-acclimation dramatically increased cold tolerance. Furthermore, antioxidant enzymes activity, MDA, TSP, and proline content increased throughout the acclimation stage, whereas they declined in the de-acclimation stage. The ascorbate peroxidase, catalase, peroxidase, and superoxide dismutase activities in the leaves tissues correlated with the alterations in cold tolerance. Higher TSP, greater antioxidant enzyme activities, and more proline content together with lower MDA content in Amphisis cultivar led to relative improvement in cold tolerance capacity of this cultivar. Our results showed antioxidant enzymes activities, TSP and proline content could be useful indices to screen cold tolerance in olive cultivars.

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1. Ahmad, P., Jaleel, C. A., Salem, M. A., Nabi, G. and Sharma, S. 2010. Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress. Crit. Rev. Biotechnol., 30: 161–175.
2. Arora, R., Wisniewski, M.E. and Scorza, R. 1992. Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica L. Batsch). Plant Physiol., 99: 1562–1568.
3. Baek, K. H. and Skinner, D. Z. 2003. Alteration of antioxidant enzyme gene expression during cold acclimation of near–isogenic wheat lines. Plant Sci., 165: 1221–1227.
4. Barranco, D., Ruiz, N. and Gomez-del Campo, M. 2005. Frost tolerance of eight olive cultivars. HortScience, 40: 558–560.
5. Bates, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205–207.
6. Beauchamp, C. and Fridovich, J. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem., 44: 276–287.
7. Beck, E. H., Fettig, S., Knake, C., Hartig, K. and Bhattarai, T. 2007. Specific and unspecific responses of plants to cold and drought stress. J. Biosci., 32: 501–510.
8. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal. Biochem., 72: 248–252.
9. Cansev, A., Gulen, H. and Eris, A. 2009. Cold–hardiness of olive (Olea europaea L) cultivars in cold–acclimated and non–acclimated stages: seasonal alteration of antioxidative enzymes and dehydrin–like proteins. J. Agric. Sci. 147: 51–61.
10. Chance, B. and Maehly, S. K. 1955. Assay of catalase and peroxidase. Meth. Enzymol., 2: 764–775.
11. Das, K. and Roychoudhury, A. 2014. Reactive oxygen species (ROS) and response of antioxidants as ROS–scavengers during environmental stress in plants. Front. Environ. Sci., 2: 53.
12. Eris, A., Gulen, H., Barut, E. and Cansev, A. 2007. Annual patterns of total soluble sugars and proteins related to cold–hardiness in olive (Olea europaea L ‘Gemlik’). J. Hortic. Sci. Biotechnol., 82: 597–604.
13. Ershadi, A., Karimi, R. and Naderi Mahdei, K. 2016. Freezing tolerance and its relationship with soluble carbohydrates proline and water content in 12 grapevine cultivars. Acta Physiol. Plant., 38: 2–10.
14. Fiorino, P. and Mancuso, S. 2000. Differential thermal analysis supercooling and cell viability in organs of Olea europaea at subzero temperatures. Adv. Hort. Sci., 14: 23–27.
15. Ghasemi Soloklui, A. A., Ershadi, A. and Fallahi, E. 2012. Evaluation of cold hardiness in seven Iranian commercial pomegranates (Punica granatum L) cultivars. HortScience, 47: 1821–1825.
16. Gomez-del Campo, M. and Barranco, D. 2005. Field evaluation of frost tolerance in 10 olive cultivars. Plant Gen. Res., 3: 385–390.
17. Guy, C. L. 1990. Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu. Rev. Plant Physiol. Plant Mol. Biol., 41: 187–223.
18. Hashempour, A., Ghasemnezhad, M., Fotouhi Ghazvini, R. and Sohani, M. M. 2014. Olive (Olea europaea L) freezing tolerance related to antioxidant enzymes activity during cold acclimation and non acclimation. Acta Physiol. Plant., 36: 3231–3241.
19. Hayat, S., Hayat, Q., Alyemeni, M. N., Wani, A. S., Pichtel, J. and Ahmad, A. 2012. Role of proline under changing environments: A review. Plant Signal Behav., 7: 1456–1466.
20. Heath, R. L. and Parker, L. 1968. Photoperoxidation in isolated chloroplasts I Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125: 189–198.
21. Karabal, E., Yücel, M. and Öktem, H. A. 2003. Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity. Plant Sci., 164: 925–933.
22. Korn, M., Peterek, S., Mock, H. P., Heyer, A. G. and Hincha, D. K. 2008. Heterosis in the freezing tolerance and sugar and flavonoid contents of crosses between Arabidopsis thaliana accessions of widely varying freezing tolerance. Plant Cell Environ., 31: 813–827.
23. Krishna, C., Keshavkant, K. S. and Naithani, S. 2000. Changes in total protein and protease activity in dehydrating recalcitrant sal (Shorea robusta) seeds. Silva Fenn., 34: 71–77.
24. Luo, L., Lin, S. Z., Zheng, H. Q., Lei, Y., Zhang, Q. and Zhang, Z. Y. 2007. The role of antioxidant system in freezing acclimation–induced freezing resistance of Populus suaveolens cuttings. For. Stud. China, 9: 107–113.
25. Macek, P., Klimeš, L., Adamec, L., Doležal, J., Chlumská, Z., de Bello, F. and Řeháková, K. 2012. Plant nutrient content does not simply increase with elevation under the extreme environmental conditions of Ladakh NW Himalaya. Arct. Antarct. Alp. Res., 44: 62–66.
26. Mancuso, S. 2000. Electrical resistance changes during exposure to low temperature measure chilling and freezing tolerance in olive tree (Olea europaea L) plants. Plant Cell Environ., 23: 291–299.
27. Nakano, Y. and Asada, K. 1980. Spinach chloroplasts scavenge hydrogen peroxide on illumination. Plant Cell Physiol., 21: 1295–1307.
28. Ortega–Garcý´, A. F. and Perago´n, J. 2009. The response of phenylalanine ammonia–lyase polyphenol oxidase and phenols to cold stress in the olive tree (Olea europaea L cv Picual). J. Sci. Food Agric., 89: 1565–1573.
29. Palliotti, A. and Bongi, G. 1996. Freezing injury in the olive leaf and effects of mefluidide treatment. J. Hortic. Sci., 71: 57–63.
30. Passardi, F., Penel, C. and Dunand, C. 2004. Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci., 9: 534–540.
31. Racchi, M. L. 2013. Antioxidant defenses in plants with attention to Prunus and Citrus spp. Antioxidants, 2: 340–369.
32. Seppanen, M. M. and Fagerstedt, K. 2000. The role of superoxide dismutase activity in response to cold acclimation in potato. Physiol. Plant., 108: 279–285.
33. Sharma, P., Jha, A. B., Dubey, R. S. and Pessarakli, M. 2012. Reactive oxygen species oxidative damage and antioxidative defense mechanism in plants under stressful conditions. J. Bot., 2012: 245–279.
34. Simkeshzadeh, N., Mobli, M., Etemadi, N. and Baninasab, B. 2011. Assessment of the frost resistance in some olive cultivars using visual injuries and chlorophyll fluorescence. J. Hortic. Sci., 2: 163–169 (article in Persian with an abstract in English).
35. Sudhakar, C., Lakshmi, A. and Giridarakumar, S. 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L) under NaCl salinity. Plant Sci., 161: 613–619.
36. Suzuki, N. and Mittler, R. 2006. Reactive oxygen species and temperature stresses: a delicate balance between signaling and destruction. Physiol. Plant., 126: 45–51.
37. Turhan, E., Aydogan, C., Baykul, A., Akoglu, A., Evrenosoglu, Y. and Ergin, S, 2012. Apoplastic antioxidant enzymes in the leaves of two strawberry cultivars and their relationship to cold–hardiness. Not. Bot. Horti. Agrobot. Cluj-Na, 40: 114–122.
38. Verbruggen, N. and Hermans, C. 2008. Proline accumulation in plants: a review. Amino Acids, 35: 753–759.
39. Vitasse, Y., Lenz, A. and Körner, C. 2014. The interaction between freezing tolerance and phenology in temperate deciduous trees. Front. Plant Sci., 5: 541.
40. Zhao, X. X., Huang, L. K., Zhang, X. Q., Li, Z. and Peng, Y. 2014. Effects of heat acclimation on photosynthesis antioxidant enzyme activities and gene expression in orchard grass under heat stress. Molecules, 19: 13564–13576.