Ectoines Mitigate the Reduction of Antagonistic Activity of Bacteria against Phytophthora drechsleri Tucker in Saline Conditions

Document Type : Original Research

Authors
A. Kamaly 1
A. Sadeghi 2
Z. Arghand 3
M. Ahmadzadeh 1
1 Department of Plant Protection, Faculty of Agricultural Science and Engineering, University of Tehran, Islamic Republic of Iran.
2 Department of Microbial Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Islamic Republic of Iran.
3 Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, Pajuhesh Science and Technology Park, Pajuhesh Boulevard, Tehran, Islamic Republic of Iran.
Abstract
The objective of the present study was to evaluate antifungal activities of 18 native Bacillus strains against Phytophthora drechsleri Tucker in saline and normal conditions. Besides, the effect of exogenously provided bacterial osmoprotectants (ectoines) on biocontrol activity of three selected Bacillus strains with the highest biocontrol activity and three antagonistic bacteria from two different genera (Pseudomonas fluorescens, Streptomyces rimosus and Streptomyces monomycini) was studied in normal and saline conditions. To reveal the effects of ectoines on the mode of action of antagonism, amylase, protease, lipase, cellulase, chitinase and Hydrogen Cyanide (HCN) activity, biofilm formation and intracellular ectoines of the selected strain (Bacillus amyloliquefaciens UTB96) were investigated in normal and saline conditions. Phylogenetic tree based on the 16S rRNA gene sequences divided Bacillus strains into two groups: one clade included strains that were tolerant up to 5% and the second 13% NaCl. Salt (0.3M NaCl) reduced the antagonistic activity of selected Bacillus strains (10.39-38.34%) and P. fluorescens (25.77%) compared to the control. Exogenously provided ectoines adjusted the biocontrol drop caused by NaCl in Bacillus and Pseudomonas strains and increased biocontrol activity of S. monomycini. Salinity (0.3M NaCl) reduced amylase activity of UTB96 up to 17% and ectoines prevented the reduction. Salt also decreased biofilm formation to about 3-fold and ectoines significantly ameliorated the reduction. The HPLC assay indicated that UTB96 accumulated ectoine and hydroxyectoine 0.16 and 0.10 µg/mg cell dry weight, respectively. Exogenously added ectoine and hydroxyectoine led to a significant increase in UTB96 intracellular ectoines concentrations.

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Journal of Agricultural Science and Technology
Volume 22, Issue 4
May and June 2020
Pages 1083-1098