Does Wolbachia Change Diapause and Energy Reserves of Trichogramma brassicae in Response to Light Wavelengths?

Authors
Department Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Islamic Republic of Iran.
Abstract
The present study examined the light wavelengths effect on the diapause percentage of progeny and energy reserves of maternal generation in sexual and asexual Trichogramma brassicae that had been reared under different light wavelengths before oviposition. Photoperiod has a maternal effect on the diapause induction in Trichogramma wasps; however, the light wavelengths effect on their diapause has not been studied. In this study, we reared the maternal generation of both strains under five light wavelengths including blue (455~475 nm), green (515~535 nm), orange (585~595 nm), red (620~630 nm), and white (5,000~10,000 K), and allowed 24 hours old females to oviposit in Ephestia kuehniella eggs. The diapausing generation was placed at 10°C and absolute darkness for two months. The results showed that Wolbachia infection and light wavelengths had significant effects on the diapause percentage and energy reserves of T. brassicae, excepting glycogen contents. The maximum and minimum diapause percentage was observed under green and white light in asexual, and under white and green light in sexual strain. The data showed that the sexual strain had lower lipid and protein levels than the asexual strain, except when exposed under white light. The diapause percentage in the sexual strain was higher than in the asexual strain under all light wavelengths, and the reaction of parasitoids toward light wavelengths was different in the two strains. Therefore, Wolbachia can cause a different reaction to light wavelengths in both diapause percentage and pattern of the parasitoid. These results should be considered to improve mass-rearing and long-term storage of this parasitoid.

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1. Amiri, A. and Bandani, A. R. 2013. Comparison of Energy Reserves in Prediapause and Diapausing Adult Sunn Pest, Eurygaster integriceps Puton (Hemiptera: cutelleridae). J. Agr. Sci. Tech., 15: 435-444.
2. Bailey, E. 1975. Biochemistry of Insect Flight. Part 2. Fuel Supply. In: “Insect Biochemistry and Function”, (Eds.): Candy, D. J. and Kilby, B. A. Chapman and Hall, London, UK, PP. 89-176.
3. Bashan, M., Akbas, H. and Yurdakoc, K. 2002. Phospholipid and Triacylglycerol Fatty Acid Composition of Major Life Stages of Sunn Pest, Eurygaster integriceps (Heteroptera: Scutelleridae). Comp. Biochem. Physiol., 132: 375-380.
4. Beck, S. D. 1980. Insect Photoperiodism. 2nd Edition, Academic Press, New York, London, 387 PP
5. Beenakkers, A. M. T., Van der Horst, D. J. and Van Marrewijik, W. J. A. 1981. Role of Lipids in Energy Metabolism. In: “Energy Metabolism in Insects”, (Ed.): Downer, R. J. H. Plenum Press, New York, USA.
6. Bertholf, L. M. 1940. Reactions to Light in Insects. Bios., 11: 39-43.
7. Bordenstein, S. R. and Werren, J. H. 2000. Do Wolbachia Influence Fecundity in Nasonia vitripennis? Heredity, 84: 54-62.
8. Bradford, M. M. 1976. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Proteins Utilizing the Principle of Protein Dye Binding. Anal. Biochem., 72: 248-254.
9. Briegel, H. 1990. Metabolic Relationship between Female Body Size, Reserves, and Fecundity of Aedes aegypti. J. Insect Physiol., 36: 165-172.
10. Bunning, E. and Joerrens, G. 1960. Tagesperiodishe Antagonistische Schwankungen der Blau-Violett-und Gelbrot-Empfindlichkeit als Grundlage der Photoperiodischen Diapause-Induktion bei Pieris brassicae. Zeitschrift für Naturforschung., 15: 205-213.
11. Costas, L. A. 1951. The Effect of Varying Conditions on Oviposition by Trichogramma on Eggs of Angoumuis Grain Moths. J. Econ. Entomol., 34: 57-58.
12. Denlinger, D. L. 1991. Relationship between Cold Hardiness and Diapause. In: “Insect at Low Temperature”, (Eds.): Lee, R. E. and Denlinger D. L. Chapman and Hall, New York, USA, PP. 174-198.
13. Foray, V., Pelisson, P. F., Bel-Venner, M. C., Desouhant, E., Venner, S., Menu, F., Giron, D. and Rey, B. 2012. A Handbook for Uncovering the Complete Energetic Budget in Insects: The van Handel’s Method (1985) Revisited. Physiol. Entomol., 37: 295-302.
14. Garcia, P. V., Wajnberg, E., Pizzol, J. and Olivejra, M. L. M. 2002. Diapause in the Egg Parasitoid Trichogramma cordubensis: Role of Temperature. J. Insect Physiol., 48: 349-355.
15. Hamdorf, K., Schwemer, J. and Gogala, M. 1971. Insect Visual Pigment Sensitive to Ultraviolet Light. Nature., 231: 458-459.
16. Han, R. D., Gan, Y. L., Kong, X. H. and Ge, F. 2008. Physiological and Endocrine Differences between Diapausing and Non-Diapausing Larvae of the Pine Caterpillar Dengrolimus tabulaeformis (Lepidoptera: Lasiocampidae). Zool. Stud., 47(1): 96-102.
17. Han, R. D., Ge, F., Yardim, N. E. and He, Z. H. 2005. The Effect of Low Temperatures on Diapause and Non-Diapause Larvae of Pine Caterpillar Dengrolimus tabulaeformis (Lepidoptera: Lasiocampidae). Appl. Entomol. Zool., 40: 429-435.
18. Harris, F. A., Lloyd, E. P., Lane, H. C. and Burt, E. C. 1967. Influence of Light on Diapause in the Boll Weevil I. Dependence of Diapause Response on the Spectral Composition of the Light Used to Extend the Photoperiod. J. Econ. Entomol., 60: 1565-1567.
19. Hines, W. J. W. and Smith, M. J. H. 1963. Some Aspects of the Intermediary Metabolism in the Desert Locust, Schistocerca gregaria Forskal. J. Insect Physiol., 9: 463-468.
20. Inagaki, S. and Yamashita, O. 1986. Metabolic Shift from Lipogenesis to Glycogenesis in the Last Instar Larval Fat Body of the Silkworm, Bombyx mori. Insect Biochem., 16: 327-331.
21. Ismail, M. S. M., AboGhalia, A. H., Soliman, M. F. M. and Ghallab, M. M. A. 2011. Certain Effects of Different Spectral Colors on Some Biological Parameters of the Two-Spotted Spider Mite, Tetranychus urticae. Egyp. Acad. J. Biol. Sci., 3(1): 27-39.
22. Ivanov, M. F. and Reznik, S. Ya. 2008. Photoperiodic Regulation of the Diapause of the Progeny in Trichogramma embryophagum Htg. (Hymenoptera, Trichogrammatidae): Dynamics of Sensitivity to Photoperiod at Immature Stages of Maternal Females. Annu. Rev. Entomol., 88(3): 261-268.
23. Keller, M. A. 1986. Overwintering by Trichogramma exiguum in North Carolina. Environ. Entomol., 15: 659-661.
24. Khani, A., Moharamipour, S. and Barzegar, M. 2007. Cold Tolerance and Trehalose Accumulation in Overwintering Larvae of the Codling Moth, Cydia pomonella (Lepidoptera: Tortricidae). Eur. J. Entomol., 104: 385-392.
25. Kostal, V. 2006. Eco-Physiological Phases of Insect Diapause. J. Insect Physiol., 52: 113-127.
26. Ma, C. S. and Chen, Y. W. 2006. Effects of Constant Temperature, Exposure Period, and Age on Diapause Induction in Trichogramma dendrolimi. Biol. Control., 36: 267-273.
27. Niaqi, E. N., Olembo, N. K. and Pearson, D. J. 1992. Proline Transport by Tsetse Fly Glossina morsitans Flight Muscle Mitochondria. Comp. Biochem. Physiol. B Comp. Biochem., 102(3): 579-584.
28. Pintureau, B., Lassabliere, F., Daumal, J. and Grenier, S. 2002. Does a Cyclic Natural Thermal Cure Occur in Wolbachia-Infected Trichogramma Species? Ecol. Entomol., 27: 366-372.
29. Pizzol, J. and Pintureau, B. 2008. Effect of Photoperiod Experienced by Parents on Diapause Induction in Trichogramma cacoeciae. Entomol. Exp. Appl., 127: 72-77.
30. Poorjavad, N. 2011. Morphological, Molecular and Reproductive Compatibility Studies on the Systematic of the Genus Trichogramma Westwood (Hymenoptera: Trichogrammatidae) in Tehran and Mazandran Provinces (Iran). PhD. Dissertation, University of Tehran, Iran.
31. Rambabu, J. P. and Rao, M. B. 1994. Effect of Organochlorine and Three Organophosphate Pesticides on Glucose, Glycogen, Lipid and Protein Contents in Tissues of the Freshwater Snail Bellamya dissimilis (Muller). Bull. Environ. Contam. Toxicol., 53: 142-148.
32. Reznik, S. Ya., Voinovich, N. D. and Vaghina, N. P. 2011. Maternal Influence on Diapause Induction in Trichogramma (Hymenoptera, Trichogrammatidae): the Dynamics of Pphotosensitivity. J. Appl. Entomol., 135(6): 438-445.
33. Ripfel, J. and Becker, J. 1982. Light-Dependent Mating of Drosophila subobscura and Species Discrimination. Behav. Genet., 12(3): 241-260.
34. Sancho, E., Ferrando, M. D., Fernandez, C. and Andreu, E. 1998. Liver Energy Metabolism of Anguilla anguilla after Exposure to Fenitrothion. Ecotoxicol. Environ. Saf., 41: 168-175.
35. Sorokina, A. P. and Maslennikova, V. A. 1987. Temperature Optimum for Diapause Induction in Species of the Genus Trichogramma Westw. (Hymenoptera, Trichogrammatidae). Entomol. Obozr., 66(4): 689-699.
36. Stouthamer, R. and Werren, J. R. 1993. Microbes Associated with Parthenogenesis in Wasps of the Genus Trichogramma. J. Invertebr. Pathol., 61: 6-9.
37. Storey, J. M. and Storey, K. B. 1983. Regulation of Cryoprotectant Metabolism in the Overwintering Gall Fly Larva, Eurosta solidaginis: Temperature Control of Glycerol and Sorbitol Levels. J. Comp. Physiol., 149: 495-502.
38. van Handel, E. 1965. Microseparation of Glycogen, Sugars, and Lipids. Anal. Biochem., 11: 266-271.
39. van Handel, E. 1985a. Rapid Determination of Glycogen and Sugars in Mosquitoes. J. Am. Mosq. Control Assoc., 1: 299-301.
40. van Handel, E. 1985b. Rapid Determination of Total Lipids in Mosquitoes. J. Am. Mosq. Control Assoc., 1: 302-304.
41. Venkatesh, K. and Morrison, P. E. 1980. Studies of Weight Changes and Amount of Food Ingested by the Stable Fly, Stomoxys calcitrans (Diptera: Muscidae). Can. Entomol., 112: 141-49.
42. Voinovich, N. D., Vaghina, N. P. and Reznik, S. Y. 2013. Comparative Analysis of Maternal and Grand-Maternal Photoperiodic Responses of Trichogramma Species (Hymenoptera: Trichogrammatidae). Eur. J. Entomol., 110(3): 451-460.
43. Williams, C. M., Adkisson, P. L. and Walcotts, C. 1965. Physiology of Insect Diapause. XV. The Transmission of Photoperiod Signals to the Brain of the Oak Silkworm, Antheraea pernyi. Biol. Bull., 128(3): 497-507.
44. Yaginuma, T. and Yamashita, O. 1978. Polyol Metabolism Related to Diapause in bombyx Eggs: Different Behavior of Sorbitol from Glycerol during Diapause and Post Diapause. J. Insect Physiol., 24: 347-354.
45. Zaslavski, V. A. and Umarova, T. Ya. 1981. Photoperiodic and Temperature Control of Diapause in Trichogramma evanescens Westw. (Hymenoptera, Trichogrammatidae). Entomol. Obozr., 60(4): 721-731.
46. Zaslavski, V. A. and Umarova, T. Ya. 1990. Environmental and Endogenous Control of Diapause in Trichogramma Species. Entomophaga., 35: 23-29.
47. Zhou, G., Pennington, J. E. and Wells, M. A. 2004. Utilization of Pre-Existing Energy Stores of Female Aedes aegypti Mosquitoes during the First Gonotrophic Cycle. Insect Biochem. Mol. Biol., 34: 919-925.