Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes

Document Type : Original Research

Authors
M. E. Bayram 1
K. Z. Korkut 2
1 Maize Research Institute, 54580 Sakarya, Turkey.
2 Department of Field Crops, Faculty of Agriculture, Namık Kemal University, 59030 Tekirdağ, Turkey.
Abstract
Breeding high-quality wheat cultivars is one of the main targets of breeding programs. Identification of High Molecular Weight Glutenin Subunits (HMW-GS) and Low Molecular Weight Glutenin Subunits (LMW-GS) alleles that confer high quality may be one of the easy ways to determine promising wheat lines in early generations. HMW-GS and LMW-GS alleles of 64 bread wheat (Triticum aestivum L.) genotypes from a Turkish breeding program including advanced lines and cultivars were identified using Sodium-Dodecyl-Sulfate PolyAcrylamide Gel (SDS-PAGE) electrophoresis method. In total, 26 alleles were identified including three alleles at the Glu-A1 locus, 6 alleles at the Glu-B1 locus, 2 alleles at the Glu-D locus, 6 alleles at the Glu-A3 locus, 7 at the Glu-B3 locus, and 2 alleles at the Glu-D3 locus. The most prevalent alleles among the 64 genotypes in the study were 2* (67.2%), 7+9 (42.2%), 5+10 (68.8%), A3c (28.1%), B3b (35.9%), and D3c (92.2%). The previously announced good flour quality conferring alleles 1, 17+18, 5+10 at Glu-1 loci and A3b, A3c, B3b, B3c, B3d, B3g at Glu-3 loci were also identified in the present study, indicating that these alleles can be used as markers for selection of higher-quality genotypes in wheat breeding programs. Introduction of the allele structure of the studied genotypes may enlighten the way for the wheat breeders in the crossing decisions.

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Journal of Agricultural Science and Technology
Volume 21, Issue 4
July and August 2019
Pages 981-992