Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS

Document Type : Original Research

Authors
Z. Karami 1
S. H. Peighambardoust 1
J. Hesari 1
B. Akbari-Adergani 2
1 Department of Food Science, College of Agriculture, University of Tabriz, Tabriz 5166616471, Islamic Republic of Iran.
2 Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran 11136-15911, Islamic Republic of Iran.
Abstract
The antioxidant capacity of wheat germ protein hydrolyzed by Alcalase was optimized using Response Surface Methodology (RSM). The optimum hydrolyzing parameters were found at temperature of 52.28°C, time 233 minutes, and E/S 1.46 %. The amino acids profiles of intact and hydrolyzed proteins showed that Wheat Germ Protein Hydrolysate (WGPH) had higher percentage of hydrophobic amino acids than that of intact protein. WGPH prepared in optimum condition was fractionated by RP-HPLC. The obtained fractions were subjected to ABTS assay for antioxidant capacity evaluation. The fraction with higher antioxidant value was then exposed to further analysis by LC-ESI/MS/MS. The sequences of the peptides were found to be TVGGAPAGRIVME (1257.66 Da) and GNPIPREPGQVPAY (1494.77 Da).

Keywords

Subjects

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Journal of Agricultural Science and Technology
Volume 21, Issue 4
July and August 2019
Pages 829-844