Polyphasic Approach Used for Distinguishing Fusarium temperatum from Fusarium subglutinans

Authors
J. Levic 1
F. Munaut 2
J. Scauflaire 2
S. Stankovic 1
D. Ivanovic 1
V. Krnjaja 3
1 Maize Research Institute, Zemun Polje, Belgrade-Zemun, Belgrade, Republic of Serbia.
2 Earth and Life Institute, Laboratory of Mycology, Mycology of the Catholic University of Louvain, Catholic University of Louvain, Belgium.
3 Institute for Animal Husbandry, Belgrade-Zemun, Republic of Serbia.
Abstract
Morphological, biological, and phylogenetic approaches were undertaken for the identification of pathogenic species F. temperatum in the Serbian population of F. subglutinans collected in the 1999-2010 period from Zea mays (3 root, 15 stalk, and 6 seed samples), Sorghum bicolor (two seed samples), Hordeum vulgare (one seed sample) and Taraxacum officinale (one seed sample). Based on interspecies mating compatibility analyses and the maximum parsimony analysis of EF-1α sequences, only two strains, originating from S. bicolor seed (MRIZP 0418 and MRIZP 0552), were identified as F. temperatum, while the remaining 26 single-spore strains were identified as F. subglutinans Group 2. In situ detached barley leaf assay and artificially stalk and ear inoculation of two maize hybrids demonstrated that both F. temperatum and F. subglutinans strains were medium and strong pathogens under laboratory and field conditions, respectively. These are the first data on the F. temperatum as seed-borne pathogens of sorghum, as well as pathogenicity of F. temperatum strains on maize.

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Journal of Agricultural Science and Technology
Volume 21, Issue 1
January and February 2019
Pages 221-232