Prevalence, Identification, and Molecular Variability of Potato Cyst Nematodes in Algeria

Authors
A. Mezerket 1
M. Hammache 1
C. Cantalapiedra-Navarrete 2
P. Castillo 2
J. E. Palomares-Rius 2
1 National Upper School of Agronomy (ENSA), El Harrach, Algiers, Algeria.
2 Institute of Sustainable Agriculture (IAS), Higher Council for Scientific Research (CSIC), Menéndez Pidal s / n, 14004 Córdoba, Spain.
Abstract
The aim of the present study was to evaluate the geographical distribution, infestation degree, and diversity of Potato Cysts Nematode (PCN) in Algeria, including the southern regions. Accurate identification of PCN is essential to determine the appropriate control methods to be used in an Integrated Pest Management program. PCNs were found in forty percent (12 out of thirty) of localities sampled. The average population density of PCN was much higher in the southern regions, compared to the northern regions (9.8 cysts per 100 cm3 vs.4.6 cysts per 100 cm3). The southern potato production areas were more infested with PCN than those of the north (7 from 25 fields in North vs. 5 from 5 fields in the South). Globodera pallida occurred predominantly in the northern region of Algeria, whereas G. rostochiensis occurred predominantly in the southern regions. No mixtures of these species were found in any of the positive studied localities. These species were confirmed by the molecular analysis based on PCR with species-specific primers, ITS-rDNA, and cytochrome b of mtDNA. The low molecular diversity and their phylogenetic association with the European populations of PCN suggest that Algerian populations were probably introduced from Europe, probably by infested seed-potato.

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1. Abidou, H., Valetten, S., Gauthier, P., Rivoal, R., El-Ahmed, A. and Yahyaoui, A. 2005. Molecular Polymorphism and Morpho-Metrics of Species of the Heterodera avenae Group in Syria and Turkey. J. Nematol., 37: 146-154.
2. Ashouri, A. 2007. Interactions of Transgenic-Bt Potato Resistance to Colorado Potato Beetle with the Fitness and Behavior of the Potato Aphid Macrosiphum euphorbiae. J. Agr. Sci. Tech., 9: 219-226.
3. Algerian Ministry of Agriculture Statistics, 2014. Statistiques Agricoles: Superficie et Production. Algeria, B: 64 PP.
4. Bates, J. A., Taylor, E. J., Gans, P. T. and Thomas, J. E. 2002. Determination of Relative Proportions of Globodera Species in Mixed populations of Potato Cyst Nematode Using PCR Product Melting Peak Analysis. Mol. Plant Pathol., 3:153-161.
5. Bulman, S. R. and Marshall, J. W. 1997. Differentiation of Australasian Potato Cyst Nematode (PCN) Populations Using the Polymerase Chain Reaction (PCR). N. Z. Crop Hort. Sci., 25: 123-129.
6. Cayrol, J. C., Djian, C., Panchaud-Mattei, E., Fankowski, J. P. and Pijarowski, L. 1992. Les Nematodes Phytoparasites: Possibilities Actuelles et Perspectives. Bull. Info. Zool., 7: 56-62.
7. Darriba, D., Taboada, G. L., Doallo, R. and Posada, D. 2012. jModel Test 2: More Models, New Heuristics and Parallel Computing. Nat. Method., 9: 772.
8. Donn, S., Griffiths, B. S., Neilson, R. and Daniel, T. J. 2008. DNA Extraction from Soil Nematodes for Multi-Sample Community Studies. Appl. Soil Ecol., 38: 20-26.
9. Dover, G. 1982. Molecular Drive: A Cohesive Mode of Species Evolution. Nature, 299: 111-117.
10. Fenwick, D. W. 1940. Methods for the Recovery and Counting of Cysts of Heterodera schachtii from Soil. J. Helminthol., 18: 155-172.
11. Frezal, P. 1954. Importance et Répercussions de la Contamination de l'Algérie par le Nematode Doré (Heterodera rostochiensis Wooll. [Woll.]. Comptes Rendus des Séances de l'Académie d’Agriculture France, 40: 71-74.
12. Fullaondo, A., Barrena, E., Viribay, M., Barrena, I., Salazar, A. and Ritter, E. 1999. Identication of Potato Cyst Nematode Species Globodera rostochiensis and G. pallida by PCR Using Specific Primer Combinations. Nematology, 1: 157-163.
13. Hall, T. A. 1999. BioEdit: A User Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98NT. Nucleic Acids Symp. Ser., 41: 95-98.
14. Howlett, B. J., Brownlee, A. G., Guest, D. L, Adcock, G. J. and McFadden, G. I. 1992. The 5S Ribosomal RNA Genesis Linked to Large and Small Subunit Ribosomal RNA Genes in the Oomycetes, Phytophthora vignae, P. cinnamonii, P. megasperma f. sp. glvcinea and Saprolegnia ferax. Curr. Genet., 22: 455-461.
15. Ijani, A. S. M., Magabala, R. B. and Nchimbi-Msolla, S. 2000. Efficacy of Different Control Methods Applied Separately in a Combination in Managing Root-Knot Nematodes (Meloidogyne spp.) in Common Beans. Eur. J. Plant Pathol., 106: 1-10.
16. Janssen, G. J. W., Janssen, R., van Norel, A., Verkerk-Bakker, B. and Hoogedoom, J. 1998. Expression of Resistance of the Root-Knot Nematodes Meloidogyne hapla and M. Fallax in Wild Solanum spp. under Field Conditions. Eur. J. Plant Pathol., 102: 869-865.
17. Jones, F. G. W. 1979. The Problems of Race-Specificity in Plant Resistance Breeding. Proceedings of the 1979 British Crop Protection Conference Pests and Diseases, 10th British Insecticide and Fungicide Conference, 19-22 November 1979, Brighton, UK, 3: 741-752.
18. Joyce, S. A., Reid, A., Driver, F. and Curran, J. 1994. Application of Polymerase Chain Reaction (PCR) Methods to Identification of Entomopathogenic Nematodes. In: "COST 812 Biotechnology: Genetics of Entomopathogenic Nematode-Bacterium Complexes". (Eds.): Burnell, M., Ehlers, R. -U and Masson, J. P. Proceedings of Symposium and Workshop, St. Patrick's College, Maynouth, County Kildare, Ireland, Luxembourg: Euro. Commi., 12: 178-187.
19. Kaczmarek, A., MacKenzie, K., Kettle, H. and Blok, V. C. 2014. Influence of Soil Temperature on Globodera rostochiensis and Globodera pallida. Phytopathol. Mediterr., 53: 396-405.
20. Katoh, K. and Standley, D. M. 2013. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Mol. Bio. Evol., 30: 772-780.
21. Madani, M., Vovlas, N., Castillo, P., Subbotin, S. A., Moens, M. 2004. Molecular Characterization of Cyst Nematode Species (Heterodera spp.) from the Mediterranean Basin Using RFLPs and Sequences of ITS-rDNA. J. Phytopathol., 15: 229-234.
22. Madani, M., Subbotin, S. A., Ward, L. J., Li, X. and De Boer, S. H. 2010. Molecular Characterization of Canadian Populations of Potato Cyst Nematodes, Globodera rostochiensis and G. pallida Using Ribosomal Nuclear RNA and Cytochrome b Genes. Can. J. Plant Pathol., 32: 252-263.
23. Mansoori, B. and Smith, C. J. 2005. Verticillium-Toxins: Their Role in Pathogenesis. J. Agric. Sci. Technol., 7: 103-114.
24. Mohammadi, M., Ghasemi, A. and Rahimian, H. 2001. Phenotypic Characterization of Iranian Strains of Pseudomonas syringae pv. syringae van Hall, the Causal Agent of Bacterial Canker Disease of Stone Fruit Trees. J. Agr. Sci. Tech., 3: 51-65.
25. Mugnièry, D. 1984. Les Nematodes de la Pomme de Terre. J. Agron., 3: 45 –50.
26. National Institute of Plant Protection. 2009. Nematode à Kystes de la Pomme de Terre Globodera rostochiensis et G. pallida, Algeria, 4 PP.
27. Norton, D. C. 1978. Ecology of Plant Parasitic Nematodes. John Wiley & Sons. New York, USA, 268 PP.
28. Picard, D., Sempere, T. and Plantard, O. 2007. A Northward Colonisation of the Andes by the Potato Cyst Nematode during Geological Times Suggests Multiple Host-Shifts from wild to Cultivated Potatoes. Mol. Phylogenet. Evol., 42: 308-316.
29. Reid, A., Evans, F., Mulholland, V., Cole, Y. and Pickup J. 2015. High throughput Diagnosis of Potato Cyst Nematodes in Soil Samples. Method. Mol. Biol., 1302: 137-148.
30. Rivoal, R., Valette, S., Bekal, S., Gauthier, J. P. and Yahyaoui, A. 2003. Genetic and Phenotypic Diversity in the Graminaceous Cyst Nematode Complex, Inferred from PCR-RFLP of Ribosomal DNA and Morphometric Analysis. Eur. J. Plant Pathol., 109: 227-241.
31. Ronquist, F. and Huelsenbeck, J. P. 2003. MRBAYES3: Bayesian Phylogenetic Inference under Mixed Models. Bioinforma., 19:1572-1574.
32. Scotto La Massese, C., 1961. Aperçu sur les Problèmes Posés par les Nématodes Phytoparasites en Algérie. Journée d’Etude et d’Information. Association de Coordination Technique Agricole, FNGPC, Paris, PP. 1-27.
33. Stone, A. R. 1973. Heterodera pallida n. sp. (Nematoda: Heterodoridae) a Second Species of Potato Cyst Nematode. Nematologica, 18: 591 –606.
34. Subbotin, S. A., Halford, P. D., Warry, A. and Perry, R. N. 2000. Variations in Ribosomal DNA Sequences and Phylogeny of Globodera Parasitizing Solanaceous Plants. Nematology, 2: 591-604.
35. Subbotin, S. A., Vierstraete, A., De Ley, P., Rowe, J., Waeyenberge, L., Moens, M. and Vanfleteren, J. R. 2001. Phylogenetic Relationships within the Cyst Forming Nematodes (Nematoda, Heteroderidae) Based on Analysis of Sequences from the ITS Region of Ribosomal DNA. Mol. Phylogenet. Evol., 21: 1-16.
36. Technical Institute for Vegetable and Industrial Crops. 2013. La Culture de la Pomme de Terre: Production et Possiblité de Transformation. Journée de la Pomme de Terre CCI Dahra Mostaghanem, 04 Decembre 2013, Algerie, 10 PP.
37. Thiéry, M. and Mugniéry, D. 1996. Interspecific rDNA Restriction Fragment Length Polymorphism in Globodera Species, Parasites of Solanaceous Plants. Fund. Appl. Nematol., 19: 471-479.
38. Thomas, W. K., Vida, J. I., Frisse, L. N. I., Mundo, M. and Baldwin, J. C. 1997. DNA Sequences from Formalin Fixed Nematodes: Integrating Molecular and Morphological Approaches to Taxonomy. J. Nematol., 29: 250-254.
39. Tirchi, N., Troccoli, A., Fanelli, E., Mokabli, A., Mouhouche, F. and De Luca, F. 2016. Morphological and Molecular Identification of Potato and Cereal Cyst Nematode Isolates from Algeria and Their Phylogenetic Relationships with Other Populations from Distant Their Geographical Areas. Eur. J. Plant. Pathol., 146: 861-880.
40. Vrain, T. C., Wakarchuk, D. A., Levesque, A. C. and Hamilton, R. I. 1992. Intraspecific rDNA Restriction Fragment Length Polymorphisms in the Xiphinema americanum group. Fund. Appl. Nematol., 15: 563-573.
41. Wendt, K. R., Vrain, T. C. and Webster, J. M. 1993. Separation of Three Species of Ditylenchus and Some Host Races of D. dipsaci by Restriction Fragment Length Polymorphism. J. Nematol., 25: 555-563.
42. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In: "PCR Protocols: A Guide to Methods and Applications", (Eds.): Innis, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J. Academic Press Inc., San Diego, USA, PP: 315–322.
43. Winslow, R. D. and Willis, R. J. 1972. Nematode Diseases of Potato in Economic Nematology. (Ed.): Webster, J. M. Academic Press Inc., London, PP. 17- 48.
44. Wollenweber, H. W. 1923. Krankheiten und Beschädigungen der Kartoffel. Arb. Forschungsinst. Kartoffelbau., 7: 1-56.
45. Wolfgong, R. 1991. Maladies et Ravageurs de la Pomme de Terre. (Ed.): Mann, Th. France, PP. 131-134.
46. Yardimci, N., Çulal Kiliç, H., Demir, Y. 2015. Detection of PVY, PVX, PVS, PVA, and PLRV on Different Potato Varieties in Turkey Using DAS-ELISA. J. Agr. Sci. Tech., 17: 757-764.
47. Zijlstra, C., Lever, A. E. M., Uenk, B. J. and van Silfhout, C. H. 1995. Differences between ITS Regions of Isolates of Root-Knot Nematodes Meloidogyne chitwoodi and M. hapla. Phytopathol., 85: 1231-1237.
Journal of Agricultural Science and Technology
Volume 20, Issue 6
November and December 2018
Pages 1293-1305