Effects of Removing Basal Fruiting Branches on Cellulose Accumulation and Activities of Cellulose Synthesis-Related Enzymes in Cotton

Authors
W. J. Zhang
Z. L. Huang
T. H. Zhou
L. Liu
L. Mi
Department of Agronomy, Anhui Agricultural University, Anhui Province, P. R. China.
Abstract
Using cotton cultivar that undergoes premature senescence in the late stage of growth, we studied the effects of removing two basal fruiting branches of two cotton varieties, namely, Quanyin-2 and Jiza-999, on the leaf area index, the SPAD value of leaf subtending cotton boll, and the development of cotton fiber in the late stage of cotton growth. We focused on the differences in cotton cellulose accumulation and fiber-related enzyme activities after removal of the basal fruiting branches and the development of cotton fiber during premature senescence. The results showed that removing the basal fruiting branches can maintain the green leaf area of the cotton canopy and the SPAD value of the subtending leaf during the late stage of reproductive growth. The period of rapid accumulation of fiber cellulose lasted longer in plants from which the basal fruiting branches had been removed, and the beginning and end of the rapid accumulation period was later than in the control group with premature senescence. The activities of cellulose synthesis-related enzymes (sucrose phosphate synthase, sucrose synthase, β-1,3-glucanase, and invertase) were higher in plants in which the basal fruiting branches had been removed than in the control group after 10 days post-anthesis. Removing the basal fruiting branches can optimize the accumulation of cellulose in cotton boll during the late growth stage and mitigate the effects of premature senescence on cellulose synthesis. We found that the peak values for cotton fiber development-related enzyme activities in the control group occurred earlier, which tended to bring cotton fiber development forward and negatively impacted fiber yield.

Keywords

Subjects

1. Amor, Y., Haigler, C. H. and Johnson, S. 1995. A Membrane-Associated Form of Sucrose Synthase and Its Potential Role in Synthesis of Cellulose and Callose in Plant. Proc. Natl. Acad. Sci. USA, 92: 9353-9357.
2. Bowling, A. J., Vaughn, K. C. and Turley R. B. 2011. Polysaccharide and Glycoprotein Distribution in the Epidermis of Cotton Ovules during Early Fiber Initiation and Growth. Protoplasma, 248: 579-90.
3. Brill, E., van Thournout, M., White, R. G., Llewellyn, D., Campbell, P. M., Engelen, S., Ruan, Y. L., Arioli, T. and Furbank, R. T. 2011. A Novel Isoform of Sucrose Synthase Is Targeted to the Cell Wall during Secondary Cell Wall Synthesis in Cotton fiber. Plant Physiol., 157: 40-54.
4. Bucher, G. L., Tarina, C., Heinlein, M., Serio, F., Jr Meins, F. and Iqlesias, V. A. 2001. Local Expression of β-1,3-glucanase Enhances Symptoms of TMV Infection in Tobacco. Plant J., 28: 361-369.
5. Cetin, O., Uzen, N. and Temiz M. G. 2015. Effect of N-Fertigation Frequency on the Lint Yield, Chlorophyll, and Photosynthesis Rate of Cotton. J. Agr. Sci. Tech. 17: 909-920.
6. Chen, J., Lv, F. J., Liu, J. R., Ma, Y. N., Wang, Y. H., Chen, B. L., Meng, Y. L. and Zhou, Z. G. 2014. Effects of Different Planting Dates and Low Light on Cotton Fiber Length Formation. Acta Physiol. Plant., 36: 2581-2595.
7. Constable, G. and Rawson, H. 1980. Carbon Production and Utilization in Cotton: Inferences from a Carbon Budget. Funct. Plant Biol., 5: 539-553.
8. Delmer, D. P. and Haigler, C. H. 2002. The Regulation of Metabolic Flux to Cellulose, a Major Sink for Carbon in Plants. Metab. Eng., 4(1) :22-28.
9. Doblin, M. S., Kurek, I., Jacok-Wilk, D. and Delmer, D. P. 2002. Cellulose Biosynthesis in Plants: from Genes to Rosettes. Plant Cell Physiol., 43:1407-1420.
10. Dong, H. Z., Niu, Y. H., Kong, X. Q. and Luo, Z. 2009. Effects of Early-Fruit Removal on Endogenous Cytokinins and Abscisic Acid in Relation to Leaf Senescence in Cotton. Plant Growth Regul., 59: 93-101.
11. Dong, H. Z., Tang, W., Li, W. J., Li, Z. H., Niu, Y. H. and Zhang, D. M. 2008. Yield, Leaf Senescence, and Cry1Ac Expression in Response to Removal of Early Fruiting Branches in Transgenic Bt Cotton. Agr. Sci. China, 7: 692-702.
12. Endler, A. and Persson, S. 2011. Cellulose Synthases and Synthesis in Arabidopsis. Mol. Plant, 42: 199-211.
13. Gormus, O., Kurt, F. and Sabagh, A. E. 2017. Impact of Defoliation Timings and Leaf Pubescence on Yield and Fiber Quality of Cotton. J. Agr. Sci. Tech., 19: 903-915.
14. Haigler, C. H., Ivanova-Datcheva, M., Hogan, P. S., Salnikov, V. V., Hwang, S., Martin, K. and Delmer, D. P. 2001. Carbon Partitioning to Cellulose Synthesis. Plant Mol. Biol., 47: 29-51.
15. Li, B., Wang, Y., Zhang, Z. Y., Wang, B. M., Eneji, A. E., Duan, L. S., Li, Z. H. and Tian, X. L. 2012. Cotton Shoot Plays a Major Role in Mediating Senescence Induced by Potassium Deficiency. J. Plant Physiol., 169: 327-335.
16. Liu, J. R., Ma, Y. N., Lv, F. J., Chen, J., Zhou, Z. G., Wang, Y. H., Abudurezike, A. K. and Oosterhuis, D. M. 2013. Changes of Sucrose Metabolism in Leaf Subtending to Cotton Boll under Cool Temperature Due to Late Planting. Field Crop. Res., 144: 200-211.
17. Liu, Y. D., Yin, Z. J., Yu, J. W., Li, J., Wei, H. L., Han, X. L. and Shen, F. F. 2012. Improved Salt Tolerance and Delayed Leaf Senescence in Transgenic Cotton Expressing the Agrobacterium IPT Gene. Biol. Plantarum, 56: 237-246.
18. Lv, F. J., Liu, J. R., Ma, Y. N., Chen, J., Abudurezikeke, A. K., Wang, Y. H., Chen, B. L., Meng, Y. L. and Zhou, Z. G. 2013. Effect of Shading on Cotton Yield and Quality on Different Fruiting Branches. Crop Sci., 53: 2670-2678.
19. Michelle, B. V. and Haigler, C. H. 2001. Sucrose-Phosphate Synthase Activity Rises in Correlation with High-Rate Cellulose Synthesis in Three Heterotrophic Systems. Plant Physiol., 127: 1234-1242.
20. Pettigrew, W. T. 1999. Potassium Deficiency Increase Specific Leaf Weights and Leaf Glucose Levels in Field-Grown Cotton. Agron. J., 91: 962-968.
21. Plenet, D., Mollier, A. and Pellerin, S. 2000. Growth Analysis of Maize Field Crops under Phosphorus Deficiency. II. Radiation-Use Efficiency, Biomass Accumulation and Yield Components. Plant Soil., 2: 259-272.
22. Ruan, Y. L., Llewellyn, D. J. and Furbank, R. T. 2003. Suppression of Sucrose Synthase Gene Expression Represses Cotton Fiber Cell Initiation, Elongation, and Seed Development. Plant Cell, 15: 952-964.
23. Ruan, Y. L., Xu, S. M., White, R. and Furbank, R. T. 2004. Genotypic and Developmental Evidence for the Role of Plasmodesmatal Regulation in Cotton Fiber Elongation Mediated by Callose Turnover. Plant Physiol., 136: 4104-4113.
24. Ruan, Y. L., Jin, Y. and Huang, J. 2009. Capping Invertase Activity by Its Inhibitor: Roles and Implications in Sugar Signaling, Carbon Allocation, Senescence and Evolution. Plant Signal. Behav., 4: 983-985.
25. Saxena, I. M. and Brown, R. M. Jr. 2005. Cellulose Biosynthesis: Current Views and Evolving Concepts. Ann. Bot., 96: 9-21.
26. Shimizu, Y., Aotsuka, S., Hasegawa, O., Sakai, F. and Hayashi, T. 1997. Changes in Levels of mRNAs for Cell Wall-Related Enzymes in Growing Cotton Fiber Cells. Plant Cell Physiol., 38: 375.
27. Shu, H. M., Zhou, Z. G., Xu, N. Y., Wang, Y. H. and Zheng, M. 2009. Sucrose Metabolism in Cotton (Gossypium hirsutum L.) Fibre under Low Temperature during Fibre Development. Eur. J. Agron., 31: 61-68.
28. Stewart, J. McD., Oosterhuis, D., Heitholt, J. J. and Mauney, J. 2010. Physiology of Cotton. Springer Ebooks, p: 80-96.
29. Tucker, M. R., Paech, N. A., Willemse, M. T. and Koltunow, A. M. 2001. Dynamics of Callose Deposition and β-1,3-Glucanase Expression during Reproductive Events in Sexual and Apomictic Hieracium. Planta, 212: 487-498.
30. Updegraff, D.M. 1969. Semimicro Determination of Cellulose in Biological Materials. Anal. Biochem. , 32: 420-424.
31. Wang, Q. H., Zhang, X., Li, F. G., Hou, Y. X., Liu, X. L. and Zhang, X. Y. 2011. Identification of a UDP-Glucose Pyrophosphorylase from Cotton (Gossypium hirsutum L.) Involved in Cellulose Biosynthesis in Arabidopsis thaliana. Plant Cell Rep., 30: 1303-1312.
32. Wright, P. R. 1999. Premature Senescence of Cotton (Gossypium hirsutum L.): Predominantly a Potassium Disorder Caused by an Imbalance of Source and Sink. Plant Soil, 211: 231-239.
33. Wullschleger, S. D. and Oosterhuis, D. M. 1990. Photosynthetic Carbon Production and Use by Developing Cotton Leaves and Bolls. Crop Sci., 30: 1259-1264.
34. Zhang, W. J., Shu, H. M., Hu, H. B., Chen, B. L., Wang, Y. H. and Zhou, Z. G. 2009. Genotypic Differences in Some Physiological Characteristics during Cotton Fiber Thickening and Its Influence on Fiber Strength. Acta Physiol. Plant., 3: 927-935.
35. Zhang, W. J., Zhou, T. H., Huang, N. N., Chen, X. H., Yi, B. L. and He, J. Y. 2013. Effect of Removal of Basal Fruiting Branches on Some Premature Senescence Characters of Different Cotton Genotypes. Acta Bot. Boreali-Occidentalia Sin., 33: 1435-1441. (in Chinese, with English Abstract).
Journal of Agricultural Science and Technology
Volume 20, Issue 3
May and June 2018
Pages 557-569