Bioactive Compounds and Volatile Profile Dynamics During Fruit Growth of Several Plums Cultivars

Authors
1 Department of Food Engineering, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Cluj, Romania.
2 Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Calea Mănăștur, 400372, Cluj-Napoca, Cluj, Romania.
Abstract
The therapeutic value of plums is provided by the contained bioactive compounds, but in consumers choice an essential role is played by the product flavour in which volatile compounds are important contributors. The content in bioactive compounds, the antioxidant activity as well as the volatile profile of three plum cultivars were determined during fruit development. In the analyzed samples, depending on cultivar, harvesting time and the position of fruit in the tree crown, the determined total phenolic content varied between 60.31–699.92 mg GAE 100 g-1, while the flavonoids and anthocyanins content ranged between 11.24–254.46 mg QE 100 g-1, and 0.09–1.65 mg CE 100 g-1, respectively. Using ITEX/GC-MS technique, there were 99 volatile compounds detected in the samples of which 93 were tentatively identified. The volatiles present in the plums cultivars included alcohols, aldehydes, ketones, esters, terpenoids, lactones and others. The most abundant class (in all plum cultivars and developmental phases) was that of aldehydes (49.40–87.01%), the main representatives being hexanal, benzaldehyde, nonanal, heptanal and 2-hexenal, with hexanal having the largest relative peak areas. The identification and quantification of volatile compounds and knowing their accumulation dynamic throughout the ripening process may allow better valorising of fruits depending on cultivar and harvesting time.

Keywords


1. Bunea, A. D., Rugină, D. O., Pintea, A. M., Sconţa, Z., Bunea, C. I. and Socaciu, C. 2011. Comparative Polyphenolic Content and Antioxidant Activities of Some Wild and Cultivated Blueberries from Romania. Not. Bot. Horti. Agrobo., 39(2): 70–76.
2. Cevallos-Casals, B.A., Byrne, D., Okie, W. R. and Cisneros-Zevallos, L. 2006. Selecting New Peach and Plum Genotypes Rich in Phenolic Compounds and Enhanced Functional Properties. J. Food Chem., 96: 273–280.
3. Chai, Q., Wu, B., Liu, W., Wang, L., Yang, C., Wang, Y., Fang, J., Liu, Y. and Li, S. 2012. Volatiles of Plums Evaluated by HS-SPME with GC–MS at the Germplasm Level. J. Food Chem., 130: 432–440.
4. Díaz-Mula, H.M., Zapata, P.J., Guillén, F., Martínez-Romero, D., Castillo, S., Serrano, M. and Valero, D. 2009. Changes in Hydrophilic and Lipophilic Antioxidant Activity and Related Bioactive Compounds during Postharvest Storage of Yellow and Purple Plum Cultivars. Postharvest Biol. Technol., 51: 354–363.
5. Dugalic, K., Sudar, R., Viljevac, M. ,. Josipovic, M, and Cupic, T. 2014. Sorbitol and Sugar Composition in Plum Fruits Influenced by Climatic Conditions. J. Agr. Sci. Tech., 16: 1145-1155
6. Giusti, M. M. and Wrolstad, R. E. 2001. Unit F1. 2: Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy. Vol. Handbook of Analytical Food Chemistry. (Ed.): Wrolstad, R. E., John Wiley and Sons, Inc., New York.
7. Giusti, M. M. and Wrolstad, R. E. 2003. Acylated Anthocyanins from Edible Sources and Their Applications in Food Systems. J. Biol. Eng., 14: 217–225.
8. Gómez, E. and Ledbetter, C. A. 1997. Development of Volatile Compounds during Fruit Maturation: Characterization of Apricot and Plum Apricot Hybrids. J. Sci. Food Agric., 74: 541–546.
9. Kim, D. O., Jeong, S. W. and Lee, C. Y. 2003a. Antioxidant Capacity of Phenolic Phytochemicals from Various Cultivars of Plums. J. Food Chem., 81: 321–326.
10. Kim, D. O., Chum, O. K., Kim, Y.J., Moon, H.Y. and Lee, C. Y. 2003b. Quantification of Polyphenolics and Their Antioxidant Capacity in Fresh Plums. J. Agric. Food Chem., 51: 6509–6515.
11. Louw, E. D. and Theron, K. I. 2012. Volatile Dynamics during Maturation, Ripening and Cold Storage of Three Japanese Plum Cultivars (Prunus salicina Lindl.). Postharvest Biol. Technol., 70: 13–24.
12. Manach, C., Scalbert, A., Morand, C., Remesy, C. and Jimenez, L. 2004. Polyphenols: Food Sources and Bioavailability. Am. J. Clin. Nutr., 79: 727–747.
13. Mihalache Arion, C., Tabart, J., Kevers, C., Niculaua, M., Filimon, R. and Beceanu, D. D. 2014. Antioxidant Potential of Different Plum Cultivars during Storage. J. Food Chem., 146: 485–491.
14. Miletic, N., Popovic, B., Mitrovic, O. and Kandic, M. 2012. Phenolic Content and Antioxidant Capacity of Fruits of Plum cv. ‘Stanley’ (Prunus domestica L.) as Influenced by Maturity Stage and On-Tree Ripening. Aust. J. Crop. Sci., 6(4): 681–687.
15. Nunes, C., Coimbra, M. A., Saraiva, J. and Rocha, S. M. 2008. Study of the Volatile Compounds of a Candied Plum and Estimation of Their Contribution to the Aroma. J. Food Chem., 111: 897–905.
16. Odriozola-Serrano, I., Soliva-Fortuny, R. and Nbelloso, O. M. 2008. Effect of Minimal Processing on Bioactive Compounds and Color Attributes of Fresh-Cut Tomatoes. Sci. Direct, LWT, 41: 217–226.
17. Pino, J. A. and Quijano, C. E. 2011. Study of the Volatile Compounds from Plum (Prunus domestica L. cv. Horvin) and Estimation of Their Contribution to the Fruit Aroma. In Ciência e Tecnologia de Alimentos, Campinas, 32(1): 76-83.
18. Prior, R. L. 2003. Fruits and Vegetables in the Prevention of Cellular Oxidative Damage. Am. J. Clin. Nutr., 78: 570–578.
19. Singleton, V. L., Orthofer, R. and Lamuela-Reventos, R. M. 1999. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Meth. Enzymol., 299: 152–178.
20. Socaci, S. A., Socaciu, C., Mureşan, C., Fărcaş, A., Tofană, M., Vicaş, S. and Pintea, A. 2014. Chemometric Discrimination of Different Tomato Cultivars Based on Their Volatile Fingerprint in Relation to Lycopene and Total Phenolics Content. Phytochem. Anal., 25: 161–169.
21. Stacewicz-Sapuntzakis, M., Bowen P. E., Hussain, E. A., Damayanti-Wood, B. I. andFarnsworth, N.R. 2001. Chemical Composition and Potential Health Effects of Prunes: A Functional Food? Crit. Rev. Food. Sci. Nutr., 41: 251–286.
22. Stohr, H., Mosel, H. D. and Herrmann, K. 1975. The Phenolics of Fruits. VII. The Phenolics of Cherries and Plum and the Changes in Catechins and Hydroxycinnamic Acid Derivatives during the Development of Fruits. Zeitschrift für Lebensmitteluntersuchung und-Forschung A., 159(2): 85–91.
23. Tomás-Barberán, F. A., Gil, M. I., Cremin, P., Waterhouse, A. L., Hess-Pierce, B. and Kader, A. A. 2001. HPLC-DAD-ESIMS Analysis of Phenolic Compounds in Nectarines, Peaches and Plums. J. Agric. Food Chem., 49: 4748–4760.
24. Veličković, J. M., Kostić, D. A., Stojanović, G. S., Mitić, S. S., Mitić, M. N., Ranđelović, S. S. and Đorđević, A. S. 2014. Phenolic Composition, Antioxidant and Antimicrobial Activity of the Extracts from Prunus spinosa L. Fruit. Hem. Ind., 68(3): 297–303.
25. Vendramini, A. L. and Trugo, L. C. 2000. Chemical Composition of Acerola Fruit (Malpighia punicifolia L.) at Three Stages of Maturity. J. Food Chem., 71: 195–198.
26. Vizzotto, M., Cisneros-Zevallos, L. and Byrne, D. H. 2007. Large Variation Found in the Phytochemical and Antioxidant Activity of Peach and Plum Germplasm. J. Am. Soc. Hort. Sci., 132: 334–340.
27. Vlaic, R. A., Mureșan, A. E., Mureșan, V., Scrob, S. A., Moldovan, O. P., Mitre, V. and Muste, S. 2014. Physico-Chemical Changes during Growth and Development of Three Plum Varieties. Bull. UASVM Food Sci. Technol., 71(2).
28. Zhishen, J., Mengcheng, T. and Jianming, W. 1999. The Determination of Flavonoid Contents in Mulberry and Their Scavenging Effects on Superoxide Radicals. Food Chem., 64: 555–559.