Effect of Roundup 360 SL on Survival of Pseudomonas sp. SP0113 Strain and Effective Control of Phytopathogens

Authors
S. W. Przemieniecki 1
T. P. Kurowski 1
M. M. Damszel 2
A. Karwowska 3
E. Adamiak 4
1 Department of Entomology, Phytopathology and Molecular Diagnostic, Faculty of Environmental Management and Agriculture, University of Warmia And Mazury, Prawocheńskiego 17, 10-720 Olsztyn, Poland.
2 University of Warmia And Mazury, Faculty of Environmental Management and Agriculture, Department of Entomology, Phytopathology and Molecular Diagnostic, Prawocheńskiego 17, 10-721 Olsztyn, Poland.
3 Department of Plant Breeding and Seed Production, Faculty of Environmental Management and Agriculture, University of Warmia And Mazury, Plac Łódzki 3, 10-724 Olsztyn, Poland.
4 Department of Agricultural Systems, Faculty of Environmental Management and Agriculture, University of Warmia And Mazury, Plac Łódzki 3, 10-718 Olsztyn, Poland.
Abstract
Studies on determination of the effect of herbicides on survivability of Plant Growth Promoting Bacteria have a strategic usefulness in determination of plant health and the fate of applied agrochemicals in agroecosystem. Antimicrobial potential was assessed using estimation of a minimum inhibitory concentration of the Roundup 360 SL against Pseudomonas sp. A quantitative analysis of bacteria was performed, and the tendency of physicochemical changes in the mineral medium was evaluated during long-term exposure to the herbicide. Furthermore, the antagonism of the SP0113 strain against F. culmorum and F. oxysporum under stress conditions caused by Roundup® 360 SL was verified. It was demonstrated that use of the undiluted and 2.6-fold diluted product resulted in the inhibition of growth of the investigated strain. Pseudomonas sp. SP0113 showed survivability and resistance to near recommended dose concentration of Roundup® 360 SL. The possibility of bacterial development on the Tryptic Soy Agar (TSA) medium at contact concentrations of 14.4 and 5.4 mg mL-1, as per the diluents quantity declared by the producer, indicates the role of cofactors such as: adiuvant or pH, redox potential (mV) or salinity. They comprise pH change, oxidation and salinity that may be due to the reaction of the active substance of the herbicide with mineral nutrient ingredients. The high salinity of environment, as a result of the reactions with the ingredients contained in the medium, is characteristic for concentrations higher than those recommended in practice. Furthermore, it was found that glyphosate limits the growth of fungi of the Fusarium genus, which support plant protection using strain SP0113.

Keywords

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Journal of Agricultural Science and Technology
Volume 19, Issue 6
November and December 2017
Pages 1417-1427