Effects of Low Temperature on Seed Germination, Early Seedling Growth and Antioxidant Systems of the Wild Elymus nutans Griseb.

Authors
J. J. Fu 1
J. Liu 1
L. Y. Yang 1
Y. J. Miao 2
Y. F. Xu 1
1 Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
2 Department of Plant Science and Technology, College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China.
Abstract
Low temperature is a limiting factor of seed germination and plant growth. In this study, two independent experiments were conducted to investigate the effects of low temperature on germination, early seedling growth and antioxidant systems in two provenances of Elymus nutans Griseb. (Damxung, DX and Gannan, GN). The seeds and early seedlings of DX were more tolerant to low temperture than those of GN. Low temperature (5oC) negatively affected seed Germination Percentage (GP), Germination Energy (GE) and the First Germination Time (FGT) in both provenances of E. nutans. Low temperature also increased Mortality Percentage (MP) when compared with control plants grown at 25oC. Low temperature treatments significantly reduced length and fresh/dry weight of shoots and roots in the two accessions after 29 days of exposure to 5oC. In the second experiment, after 5 days of low temperature treatment, DX exhibited significantly higher Chlorophyll (Chl) and Carotenoid (Car) content as well as increased activities of Peroxidase (POD), SuperOxide Dismutase (SOD), Catalase (CAT). In contrast we observed lower Electrolyte Leakage (EL) and reduction of the amounts of MalonDiAldehyde (MDA), Hydrogen peroxide (H2O2) and superoxide radical (). We conclude that GN is more susceptible to low temperature than DX due to more severe oxidative damage resulting from Reactive Oxygen Species (ROS) and lower antioxidant enzyme activities.

Keywords

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Journal of Agricultural Science and Technology
Volume 19, Issue 5
September and October 2017
Pages 1113-1125