Restricted Herbicide Translocation Was Found in Two Glyphosate-resistant Italian Ryegrass (Lolium multiflorum Lam.) Populations from New Zealand

Authors
H. Ghanizadeh 1
K. Harrington 1
T. James 2
D. Woolley 1
N. Ellison 3
1 Institute of Agriculture and Environment, Massey University, PB 11-222, Palmerston North 4442, New Zealand.
2 AgResearch Ltd., Ruakura Research Centre, PB 3123, Hamilton 3240, New Zealand.
3 AgResearch Grasslands, PB 11008, Palmerston North 4442, New Zealand.
Abstract
Glyphosate resistance has been found in two populations of Italian ryegrass (Lolium multiflorum) following many years of glyphosate application in New Zealand vineyards. Dose-response experiments showed that both glyphosate-resistant Italian ryegrass populations had 10-fold levels of resistance to glyphosate compared to a susceptible population. Possible mechanisms of glyphosate resistance target site mutation at position Pro-106 of 5-enolpyruvyl-shikimate-3-phosphate synthase gene and alterations in glyphosate absorption/translocation in these populations were investigated. Genotyping assays demonstrated that there was no point mutation at Codon 106 of the 5-enolpyruvyl-shikimate-3-phosphate synthase gene in either of the resistant populations. Glyphosate-resistant and susceptible populations did not differ in 14C-glyphosate absorption. However, in both resistant populations, much more of the absorbed 14C-glyphosate was retained in the treated leaf than occurred in the susceptible population. Significantly more 14C-glyphosate was found in the pseudostem region of susceptible plants than resistant plants. Based on these results, it was suggested that alterations in glyphosate translocation patterns plays a major role in glyphosate resistance for Italian ryegrass populations from these New Zealand vineyards.

Keywords

1. Adu-Yeboah, P., Malone, J. M., Gill, G. and Preston, C. 2014. Reduced Glyphosate Translocation in Two Glyphosate-Resistant Populations of Rigid Ryegrass (Lolium rigidum) from Fence Lines in South Australia. Weed Sci., 62: 4-10.
2. Baerson, S. R., Rodriguez, D. J., Tran, M., Feng, Y. M., Biest, N. A. and Dill, G. M. 2002. Glyphosate-Resistant Goosegrass. Identification of a Mutation in the Target Enzyme 5-Enolpyruvylshikimate-3-Phosphate Synthase. Plant Physiol., 129: 1265-1275.
3. Beecher, M., Hennessy, D., Boland, T. M., McEvoy, M., O'Donovan, M. and Lewis, E. 2015. The Variation in Morphology of Perennial Ryegrass Cultivars throughout the Grazing Season and Effects on Organic Matter Digestibility. Grass Forage Sci., 70: 19-29.
4. Bostamam, Y., Malone, J. M., Dolman, F. C., Boutsalis, P. and Preston, C. 2012. Rigid Ryegrass (Lolium rigidum) Populations Containing a Target Site Mutation in EPSPS and Reduced Glyphosate Translocation are More Resistant to Glyphosate. Weed Sci., 60: 474-479.
5. Carvalho, L. B. d., Alves, P. L., Gonzalez-Torralva, F., Cruz-Hipolito, H. E., Rojano-Delgado, A. M., Prado, R. d., Gil-Humanes, J., Barro, F. and Luque de Castro, M. D. 2012. Pool of Resistance Mechanisms to Glyphosate in Digitaria insularis. J. Agr. Food Chem., 60: 615-622.
6. Gaines, T. A., Zhang, W. ,L., Wang, D., Bukun, B., Chisholm, S. T., Shaner, D.L., Nissen, S. J., Patzoldt, W. L., Tranel, P. J., Culpepper, A. S., Grey, T. L., Webster, T. M., Vencill, W. K., Sammons, R. D., Jiang, J. M., Preston, C., Leach, J. E. and Westra, P. 2010. Gene Amplification Confers Glyphosate Resistance in Amaranthus palmeri. Proc. Natl. Acad. Sci. USA, 107: 1029-1034.
7. Ge, X., d'Avignon, D. A., Ackerman, J. J. H. and Sammons, R. D. 2010. Rapid Vacuolar Sequestration: the Horseweed Glyphosate Resistance Mechanism. Pest Manag. Sci., 66: 345-348.
8. Ge, X., d'Avignon, D. A., Ackerman, J. J. H., Collavo, A., Sattin, M., Ostrander, E. L., Hall, E. L., Sammons, R. D. and Preston, C. 2012. Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation. J. Agric. Food Chem., 60:1243-1250
9. Ge, X., d'Avignon, D. A., Ackerman, J. J. H. and Sammons, R. D. 2014. In vivo 31P-Nuclear Magnetic Resonance Studies of Glyphosate Uptake, Vacuolar Sequestration, and Tonoplast Pump Activity in Glyphosate-Resistant Horseweed. Plant Physiol., 166: 1255-1268.
10. Ghanizadeh, H., Harrington, K. C., James, T. K. and Woolley, D. J. 2013. Confirmation of Glyphosate Resistance in Two Species of Ryegrass from New Zealand Vineyards. New Zeal. Plant Protect. J., 66: 89-93.
11. Ghanizadeh, H., Harrington, K. C., James, T. K. and Woolley, D. J. 2015a. Quick tests for detecting glyphosate-resistant Italian and perennial ryegrass. New Zeal. J. Agr. Res. 58:108-120..
12. Ghanizadeh, H., Harrington, K. C., James, T. K., Woolley, D. J. and Ellison, N. W. 2015b. Mechanisms of Glyphosate Resistance in Two Perennial Ryegrass (Lolium perenne) Populations. Pest Manag. Sci., 71:1617-1622.
13. González-Torralva, F., Gil-Humanes, J., Barro, F., Brants, I. and De Prado, R. 2012. Target Site Mutation and Reduced Translocation Are Present in a Glyphosate-resistant Lolium multiflorum Lam. Biotype from Spain. Plant Physiol. Biochem., 58: 16-22.
14. Hoagland, D. R. and Arnon, D. I. 1938. The Water-culture Method for Growing Plants without Soil. Agricultural Experiment Station, Circular, California, 347 PP.
15. Knezevic, S. Z., Streibig, J. C. and Ritz, C. 2007. Utilizing R Software Package for Dose-response Studies: The Concept and Data Analysis. Weed Technol., 21: 840-848.
16. Koger, C. H. and Reddy, K. N. 2005. Role of Absorption and Translocation in the Mechanism of Glyphosate Resistance in Horseweed (Conyza canadensis). Weed Sci., 53: 84-89.
17. Lorraine-Colwill, D. F., Powles, S. B., Hawkes, T. R., Hollinshead, P. H., Warner, S. A. J. and Preston, C. 2002. Investigations into the Mechanism of Glyphosate Resistance in Lolium rigidum. Pest. Biochem. Physiol., 74: 62-72.
18. Nandula, V. K., Reddy, K. N., Poston, D. H., Rimando, A. M. and Duke, S. O. 2008. Glyphosate Tolerance Mechanism in Italian Ryegrass (Lolium multiflorum) from Mississippi. Weed Sci., 56: 344-349.
19. Ng, C. H., Wickneswari, R., Salmijah, S., Teng, Y. T. and Ismail, B. S. 2003. Gene Polymorphisms in Glyphosate-resistant and -Susceptible Biotypes of Eleusine indica from Malaysia. Weed Res., 43: 108-115.
20. Perez-Jones, A., Park, K. W., Polge, N., Colquhoun, J. and Mallory-Smith, C. A. 2007. Investigating the Mechanisms of Glyphosate Resistance in Lolium multiflorum. Planta, 226: 395-404.
21. Powles, S. B. and Preston, C. 2006. Evolved Glyphosate Resistance in Plants: Biochemical and Genetic basis of Resistance. Weed Technol., 20: 282-289.
22. Powles, S. B. and Yu, Q. 2010. Evolution in Action: Plants Resistant to Herbicides. Annu. Rev. Plant Biol., 61: 317-347.
23. Preston, C., Wakelin, A. M., Dolman, F. C., Bostamam, Y. and Boutsalis, P. 2009. A Decade of Glyphosate-resistant Lolium around the World: Mechanisms, Genes, Fitness, and Agronomic Management. Weed Sci., 57: 435-441.
24. Sammons, R. D. and Gaines, T. A. 2014. Glyphosate Resistance: State of Knowledge. Pest. Manag. Sci., 70: 1367-1377.
25. Vila-Aiub, M. M., Balbi, M. C., Distéfano, A. J., Fernández, L., Hopp, E., Yu, Q. and Powles, S. B. 2012. Glyphosate Resistance in Perennial Sorghum halepense (Johnsongrass), Endowed by Reduced Glyphosate Translocation and Leaf Uptake. Pest Manag. Sci., 68: 430-436.
26. Wakelin, A. M., Lorraine-Colwill, D. F. and Preston, C. 2004. Glyphosate Resistance in four Different Populations of Lolium rigidum Is Associated with Reduced Translocation of Glyphosate to Meristematic Zones. Weed Res., 44: 453-459.
27. Wakelin, A. M. and Preston, C. 2006. A Target-site Mutation is Present in a Glyphosate-resistant Lolium rigidum Population. Weed Res., 46: 432-440.
28. Yu, Q., Cairns, A. and Powles, S. 2007. Glyphosate, Paraquat and ACCase Multiple Herbicide Resistance Evolved in a Lolium rigidum Biotype. Planta, 225:499-513.
29. Yuan, J. S., Tranel, P. J., and Stewart, C. N. 2007. Non-target-site Herbicide Resistance: A Family Business. Trend Plant Sci., 12: 6-13.
Journal of Agricultural Science and Technology
Volume 18, Issue 4
July and August 2016
Pages 1041-1051