Heat-shock Protein Synthesis Study by Micro-chip

Author
S. Tahmasebi Enferadi
National Institute of Genetic Engineering and Biotechnology, Biotechnology of oil industries group, Tehran frees way 15 Km, Pajouhesh BLV. Zip Code: 1417863171 – P.O.BOX 14925/16, Tehran, Iran.
Abstract
Keeping constant all agronomic factors except temperature is a way to follow Heat Shock Proteins (HSPs) expression. Three different sunflower hybrids derived from the cross between an inbred line, Cms HA 89, and three different restorer inbred lines, with resistance, susceptible, and normal reactions to drought, were produced. In order to investigate the impact of temperature on protein accumulation during achene filling phase, they were cultivated in two different geographical areas: Karaj in Iran and Udine in Italy. Total protein content and structural polypeptide fractions of Seed Storage Proteins (SSPs) were determined. The analysis of HSPs was carried out by means of lab-on-chip capillary electrophoresis. It was revealed that protein accumulation in achene occurs at a greater rate during the achene filling phase, i.e. approximately 9-25 days after pollination, in all examined hybrids. Besides, the presence of a polypeptide band of 17.7 kDa supposed to be of small Heat Shock Proteins (sHSPs) family was recorded in all three hybrids grown up in hot and arid environment, Karaj, which implied independent sHSPs expression from the paternal restorer line.

Keywords

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Journal of Agricultural Science and Technology
Volume 17, Issue 6
November and December 2015
Pages 1629-1636