Effects of Different Storage Temperatures and Times on Germination and Antioxidant Responses of Jatropha curcas Seeds

Authors
S. Gao 1
R. Yan 2
F. Chen 2
1 Institute of Ecological Forestry, Faculty of Forestry, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, People’s Republic of China.
2 College of Life Sciences, Sichuan University, 610064, Chengdu, People’s Republic of China.
Abstract
The effects of different storage temperatures and times on germination rate, MalonDiAldehyde (MDA), SuperOxide Dismutase (SOD), PerOxiDase (POD) and Catalase (CAT) activities in Jatropha curcas L. seed were investigated. The results showed that germination rates of seeds stored at room temperature and 4°C for 1 month was significantly higher than those stored for 3 months at -20 and -80°C. MDA contents increased significantly for 1 and 3 months storage with decreasing temperatures. SOD activity decreased gradually down to -80°C for 1 and 3 months storage. POD activity fluctuated slightly for 1 month, but increased apparently for 3 months storage with decreasing temperatures. CAT activity declined rapidly as storage temperatures decreased, particularly for 3 months storage. A significant interaction of storage temperatures and times was found for the activity of SOD and POD, and there was no significant interaction on germination rate, MDA content, and CAT activity. Electrophoretic analysis showed that the observed changes of SOD, POD and CAT isoenzyme bands under different storage temperatures were consistent with the changes of enzyme activities assayed in extract solutions. These results suggested that SOD, POD, and CAT may be involved in regulating the level of reactive oxygen species under different storage temperatures and times.

Keywords

1. Bailly, B. 2004. Active Oxygen Species and Antioxidants in Seed Biology. Seed Sci. Res., 14: 93–107.
2. Beauchamp, C. and Fridovich, I. 1971. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal. Biochem., 44: 276–287.
3. Bettaieb, T., Mahmoud, M., Ruiz de Galarreta, J. I. and Du Jardin, P. 2007. Relation between the Low Temperature Stress and Catalase Activity in Gladiolus Somaclones (Gladiolus grandiflorus Hort.). Sci. Hort., 113: 49–51.
4. Chaitanya, K. S. K., Naithani, R. and Naithani, S. C. 2000. Ascorbic Acid Metabolism in Ageing Recalcitrant Sal (Shorea robusta Gaertn. f.) Seeds. Indian J. Exp. Biol., 38: 1031–1035.
5. Chiu, K. Y., Chen, C. L. and Sung, J. M. 2002. Effect of Priming Temperature on Storability of Primed Sh-2 Sweet Corn Seed. Crop Sci., 42: 1996–2003.
6. Debnath, M. and Bisen, P. S. 2008. Jatropha curcas L., a Multipurpose Stress Resistant Plant with a Potential for Ethnomedicine and Renewable Energy. Current Pharmaceut. Biotechnol., 9: 288–306.
7. Gao, S., Ou-yang, C., Tang, L., Zhu, J. Q., Xu, Y., Wang S. H. and Chen, F. 2010. Growth and Antioxidant Responses in Jatropha curcas Seedling Exposed to Mercury Toxicity. J. Hazard. Mater., 182: 591–597.
8. Huang, J., Liu, H., Lu, Y. M. and Xia, R. X. 2005. Effect of Salicylic Acid on the Antioxidant System in the Pulp of 'Cara cara' Navel Orange (Citrus sinensis L. Osbeck) a Different Storage Temperatures. Postharvest Biol. Technol., 47: 168–175.
9. Larrigaudière, C., Vilaplana, R., Soria, Y. and Recasens, I. 2004. Oxidative Behavior of Blanquilla Pears Treated with 1-methylcyclopropene during Cold Storage. J. Sci. Food Agric., 84: 1871–1877.
10. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. I. 1951. Protein Measurement with Folin Phenol Reagent. J. Biol. Chem., 193: 265–275.
11. Montavon, P., Kukic, K. R. and Bortlik, K. 2007. A Simple Method to Measure Effective Catalase Activities: Optimization, Validation, and Application in Green Coffee. Anal. Biochem., 360: 207–215.
12. Passardi, F., Cosio, C., Penel, C. and Dunand, C. 2005. Peroxidases Have More Functions Than a Swiss Army Knife. Plant Cell Rep., 24: 255–265.
13. Pukacka, S. and Ratajczak, E. 2005. Production and Scavenging of Reactive Oxygen Species in Fagus sylvatica Seeds during Storage at Varied Temperature and Humidity. J. Plant Physiol., 162: 873–885.
14. Ros Barcelo, A. 1987. Quantification of Lupin Peroxidase Isoenzymes by Densitometry. Anal. Biol., 14: 33–38.
15. SAS Institute. 2004. SAS Version 9.1. Statistical Software, Cary, NC, USA.
16. Sakharov, I. Y. and Bautista, G. 1999. Variation of Peroxidase Activity in Cacao Beans during Their Ripening, Fermentation and Drying. Food Chem., 65: 51–54.
17. Sung, J. M. 1996. Lipid Peroxidation and Peroxide-scavenging in Soybean Seeds during Aging. Physiol. Plant., 97: 85–89.
18. Tomonari, H., Toshinari, G., Kazumitsu, M., Keiko, I., Masaya, I. and Masahiro, M. 2009. Cryopreservation and Low-temperature Storage of Seeds of Phaius tankervilleae. Plant Biotechnol. Rep., 3: 103–109.
19. Tommasi, F., Paciolla, C., de Pinto, M. C. and De Gara, L. 2006. Effects of Storage Temperature on Viability, Germination and Antioxidant Metabolism in Ginkgo biloba L. Seeds. Plant Physiol. Biochem., 44: 359–368.
20. Woodbury, W., Spencer, A. K. and Stahmann M. A. 1971. An Improve Procedure Using Ferricyanide for Detecting Catalase Isozymes. Anal. Biochem., 44: 301–305.
21. Xin, X., Wang, W. J., Yin, G. K., Lin, J., Lu, X. X. and Jing, X. M. 2010. Freezing Sensitivity and Low Temperature Storage of Quercus acutissima and Quercus variabilis Seeds. Seed Sci. Technol., 38: 170–183.
22. Yang, L., Xu Y. and Chen F. 2007. Study on Seed Germination of Jatropha curca. Seed, 26: 88–89.
Journal of Agricultural Science and Technology
Volume 17, Issue 6
November and December 2015
Pages 1619-1628