Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation

Authors
X. Liu
J. H. Wu
Y. Gao
Y. Wu
L. Qian
Z. Wang
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republics of China.
Abstract
In this study, the milk-derived GalactoOligoSaccharides (GOS) were produced by Lactobacillus delbrueckii subsp. bulgaricus and refined by an ultrafiltration-nanofiltration continuous membrane. By further investigation, we found that the GOS product purified by gel permeation chromatography mainly contained low molecular weight disaccharide and trisaccharide, that is, 4-β-galactobiose and tri-galacto-oligosaccharides. The cellular immune activity of the purified GOS was evaluated by using Intestinal Epithelial Cells (IECs). Results showed that GOS could significantly (P< 0.05) promote IECs proliferation in a dose and time dependent manner, and the relative proliferation rate after 24 hours culture was high up to 158% at the concentration of 100 μg mL-1, which was three time the value after 4 hours culture without GOS. Moreover, the production of IL-6 was observably increased and up to 133.54 ng L-1 with addition of 100 μg mL-1 GOS. These data implied that the purified GOS might have a role in promoting the immune adjustment, which could be utilized as a novel and natural immunoregulatory agent in the field of medicine and functional food. This work also revealed that the employment of transgalactosylation activity of β-galactosidase derived from the fermentation of probiotics such as Lactobacillus delbrueckii subsp. bulgaricus would enhance the value of the milk product due to the form of GOS.

Keywords

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Journal of Agricultural Science and Technology
Volume 17, Issue 6
November and December 2015
Pages 1481-1494