Antioxidant Activity of Purslane (Portulaca oleracea L.) Seed Hydro-alcoholic Extract on the Stability of Soybean Oil

Authors
S. R. Jalali Mousavi 1
R. Niazmand 2
M. Shahidi Noghabi 2
1 Department of Food Science and Technology, Islamic Azad University, Damghan Branch, Islamic Republic of Iran.
2 Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Islamic Republic of Iran.
Abstract
In this study, the antioxidant activity and protective effects of Purslane Seed Water-Methanolic Extract (PSWME) in stabilizing SoyBean Oil (SBO) were tested. DPPH radical scavenging activity of PSWME at different concentrations varied significantly (P< 0.05), ranging from 15.41% to 79.06%. Total phenolic content in PSWME was 121.09 mg Gallic Acid Equivalent (GAE) kg-1 dw. The protective effects of PSWME at the level of 100 mg L-1 in stabilizing SBO were tested and compared to BHT in a concentration of 100 mg L-1 by measuring Peroxide Value (PV) and ThioBarbituric Acid (TBA) during accelerated storage (at 60°C). The results showed that PSWME, similar to BHT, reduced the formation of primary and secondary oxidation products in SBO. Therefore, the use of this extract as a natural antioxidant is recommended to prevent the oxidation of vegetable oils.

Keywords

1. Abd El-aal, H. A. and Halaweish, F. T. 2010. Food Preservative Activity of Phenolic Compounds in Orange Peel Extracts (Citrus sinensis L.). Lucrări Ştiinţifice, 53: 233-240.
2. Ahmadi Moghadam, Y., Piri, Kh., Bahramnejad, B. and Ghiasvand, T. 2014. Dopamine Production in Hairy Root Cultures of Portulaca oleracea (Purslane) Using Agrobacterium rhizogenes. J. Agr. Sci. Tech., 16: 409-420.
3. Ahn, J. H., Kim, Y. P. and Kim, H. S. 2012. Effect of Natural Antioxidants on the Lipid Oxidation of Microencapsulated Seed Oil. Food Control., 23: 528-534.
4. Baydar, N. G., Ozkan, G. and Yasar, S. 2007. Evaluation of the Antiradical and Antioxidant Potential of Grape Extracts. Food Control., 18: 1131-1136.
5. Besong, S. A., Ezekwe, M. O. and Ezekwe, E. I. 2011. Evaluating the Effects of Freeze-dried Supplements of Purslane (Portulaca oleracea) on Blood Lipids in Hypercholesterolemic Adults. Int. J. Nutr. Metab., 3: 43-49.
6. Chang, L., Yen, W., Huang, S. and Duh, P. 2002. Antioxidant Activity of Sesame Coat. Food Chem., 78: 347-354.
7. Demirhan, E. and Ozbek, B. 2010. Drying Kinetics and Effective Moisture Diffusivity of Purslane Undergoing Microwave Heat Treatment. Korean J. Chem. Eng., 27: 1377-1383.
8. Duh, P. D., Yen, W. J., Du, P. C. and Yen, G-C. 1997. Antioxidant Activity of Mung Bean Hulls. J. Am. Oil Chem. Soc., 74: 1059-1063.
9. Erkan, N. 2012. Antioxidant Activity and Phenolic Compounds of Fractions from Portulaca oleracea L. Food Chem., 133: 775-781.
10. Heidarzadeh, S., Farzanegi, P., Azarbayjani, M. A. and Daliri, R. 2013. Purslane Effect on GLP-1 and GLP-1 Receptor in Type 2 Diabetes. Electronic Physician, 5: 582-587.
11. Inan, O., Ozcan, M. M. and Juhaimi, F. Y. A. 2012. Antioxidant Effect of Mint, Laurel and Myrtle Leaves Essential Oils on Pomegranate Kernel, Poppy, Grape and Linseed Oils. J. Clean Prod., 27: 151-154.
12. Iqbal, S. and Bhanger, M. I. 2007. Stabilization of Sunflower Oil by Garlic Extract during Accelerated Storage. Food Chem., 100: 246-254.
13. Jayaprakasha, G. K., Selvi, T. and Sakariah, K. K. 2003. Antibacterial and Antioxidant Activities of Grape (Vitis vinifera) Seed Extracts. Food Res. Int., 36: 117-122.
14. Khomdram, S. and Devi, G. A. S. 2010. Determination of Antioxidant Activity and Vitamin C of Some Wild Fruits of Manipur. Inter. Quarterly J. Life Sci., 5: 501-504.
15. Kukic, J,. Popovic, V., Petrovic, S., Muneagi, P., Ciric, A., Stojkovic, D. and Sokovic, M. 2008. Antioxidant and Antimicrobial Activity of Cynera cardunculus Extracts. Food Chem., 107: 867-868.
16. Lim, Y. and Quah, E. 2007. Antioxidant Properties of Different Cultivars of Portulaca oleracea. Food Chem., 103: 734-740.
17. Liu, L., Howe, P., Zhou, Y., Xu, Z., Hocart, C. and Zhang, R. 2000. Fatty Acids and β-carotene in Australian Purslane (Portulaca oleracea) Varieties. J. Chromatogr. A., 893: 207-213.
18. Mehran, M. 1976. Oil Testing. First Edition, Tehran University Press, 263 PP.
19. Mohdaly, A., Smetanska, I., Ramadana, M., Sarhan, M. and Mahmoud, A. 2011. Antioxidant Potential of Sesame (Sesamum indicum) Cake Extract in Stabilization of Sunflower and Soybean Oils. Ind. Crops Prod., 34: 952-959.
20. Oliveira, L., Valentao, P., Lopes, R., Andrade, P., Bento, A. and Pereira, J. 2009. Phytochemical Characterization and Radical Scavenging Activity of Portulaca oleracea L. Leaves and Stems. Microchem. J., 92: 129-134.
21. Rinaldi, R., Luisa, M. and Colelli, G. 2010. Effect of Temperature and Exogenous Ethylene on Physiological and Quality Traits of Purslane (Portulaca oleracea L.) Leaves during Storage. Postharvest Biol. Tech., 58: 147-154.
22. Salmanian, A., Sadeghi Mahoonak, A. R., Alami, M. and Ghorbani, M. 2014. Phenolic Content, Antiradical, Antioxidant, and Antibacterial Properties of Hawthorn (Crataegus elbursensis) Seed and Pulp Extract. J. Agr. Sci. Tech., 16: 343-354.
23. Shahidi, F. and Naczk. M. 2004. Phenolic in Food and Nutraceuticals. First Edition. CRC Press, 558 PP.
24. Shaker, E. 2006. Antioxidative Effect of Extracts from Red Grape Seed and Peel on Lipid Oxidation in Oils of Sunflower. LWT, 39: 883-897
25. Shantha, N. C. and Decker, E. A. 1994. Rapid, Sensitive, Iron-based Spectrophotometric Methods for Determination of Peroxide Values of Food Lipids. J. Am. Oil Chem. Soc., 77: 21-424.
26. Simopoulos, P. 2004. Omega-3 Fatty Acid and Antioxidants in Edible Wild Plants. Biol. Res., 37: 263-277.
27. Spigno, G. and Faveri, D. M. D. 2007. Antioxidants from Grape Stalks and Marc: Influence of Extraction Procedure on Yield, Purity and Antioxidant Power of the Extracts. J. Food Eng., 78: 793-801.
28. Sultana, B., Anwar, F. and Przybylki, R. 2007. Antioxidant Potential of Corncob Extracts for Stabilization of Corn Oil Subjected to Microwave Heating. Food Chem., 104: 997-1005.
29. Taha, F., Wegdy, S. and Singer, F. 2012. Comparison between Antioxidant Activities of Phenolic Extracts from Different Parts of Peanut. Life Sci. J., 9: 207-215.
30. Teixeira, C. M., Coelho, N., Olsson, M. E., Brodelius, P. E., Carvalho, I. S. and Brodelius, M. 2009. Molecular Cloning and Expression Analysis of Three Omega-6 Desaturase Genes from Purslane (Portulaca oleracea L.). Biotechnol. Lett., 31: 1089-1101.
31. Wannes, W. A., Mhamdi, B., Sriti, J., Jemia, M. B., Ouchikh, O., Hamdaoui, G., Kchouk, M. E. and Marzouk, B. 2010. Antioxidant Activities of the Essential Oils and Methanol Extracts from Myrtle (Myrtus communis var. italica L.) Leaf, Stem and Flower. Food Chem. Toxicol., 48: 1362-1370.
32. YouGuo, C., Zongji, S. and Xiaoping, C. 2009. Evaluation of Free Radicals Scavenging and Immunity–modulatory Activities of Purslane Polysaccharides. Int. J. Biol. Macromol., 45: 448-452.
Journal of Agricultural Science and Technology
Volume 17, Issue 6
November and December 2015
Pages 1473-1480