Molecular Identification of an Isolate of Peanut Mottle Virus (PeMoV) in Iran

Authors
N. Beikzadeh 1
A. Hassani-Mehraban 2
D. Peters 3
1 Khorasan Razavi Agricultural Education Center, Technical and Vocational Higher Education Institute, Mashhad, Islamic Republic of Iran.
2 The Netherlands Food and Consumer Product Safety Authority, Section of Virology and Molecular Biology, P. O. Box: 9102, 6700 HC Wageningen, The Netherlands.
3 Department of Plant Sciences, Laboratory of Virology, Wageningen University, P. O. Box: 629, Wageningen, The Netherlands.
Abstract
Peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in Golestan province. Electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. Healthy Nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, downward leaf curling, and wrinkling of the leaves. The virus was transmitted by Myzus persicae in a non-persistent manner to healthy N. benthamiana, on which symptoms were observed two weeks later. RT-PCR using an Oligo-dT and a NIb primer set resulted in a fragment of about 1093 bp, which comprised the complete coat protein (CP) gene and 3´-non-coding region. Analysis of its CP nucleotide and amino acid sequence revealed 98-99% similarity and 95-99% identity to those of Peanut mottle virus (PeMoV) isolated from other countries, respectively. The molecular data confirmed serological, vector transmission, and electron microscopic findings on the incidence of PeMoV in Iran. Additionally, sequence and phylogenetic analyses of the CP revealed clustering of Iranian PeMoV isolate with Asian/Australian isolates.

Keywords

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Journal of Agricultural Science and Technology
Volume 17, Issue 3
May and June 2015
Pages 765-776