Antimicrobial and Anti-oxidative Effects of Methanolic Extract of Dorema aucheri Boiss.

Authors
M. Mianabadi 1
M. Hoshani 1
S. Salmanian 2
1 Department of Biology, Faculty of Science, Golestan University, Gorgan, Islamic Republic of Iran.
2 Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources,
Abstract
Dorema aucheri Boiss. (Apiaceae) is an endemic plant growing in Iran. This plant is used as food and its extracts are recommended for the treatment of a wide range of diseases. To conduct this study, leaf, stem, and flower of D. aucheri were collected from near Yasouje, Iran. Antioxidant and antimicrobialeffects of methanol extracts were measured. Further, total phenolic,flavonoid, anthocyanin, carotenoid, soluble sugar, gallicacid,chlorogenic acid,caffeicacid, andp-coumaric acid contentsof plant methanol extracts were also determined. The results showed that total phenolic, anthocyanin, and soluble sugar in the stem of D. aucheri were 22.72 mg GAE g-1 dW, 19.33 mg g-1 dW, and 6.45 mg g-1 dW respectively, greater than those of the other samples tested. Also, phenolic acids were identified by RP-HPLCand chlorogenic acid was the predominant phenolic compound in the samples. The highest amount of flavonoid (1.95 mg QE g-1 dW) was observed in the flower. All of the different extracts exhibited a good antioxidant activity based on inhibition of fatty acid oxidation assay. Themaximal inhibition was observed in the leaf (48.52%) and flower (54.24%) of D. aucheri. Ferric reducing antioxidant power (FRAP) was also high in the stem and flower. In addition, the results showed that leaf, stem, and flower extracts had inhibitory activity against four bacteria tested. The highest antimicrobial activity was obtained with flower extract. These results suggest that the methanolic extracts from different parts of D. aucheri are a valuable source of effective compounds. The antioxidant and antimicrobial activity of extracts was correlated with p-coumaric and caffeic acid content.

Keywords

1. Almajano, M. P., Carbo, R., Jimenez, J. A. and Gorden, M. H. 2008. Antioxidant and Antimicrobial Activities of Tea Infusions. Food Chem., 108: 55-63.
2. Andreasen, M. F., Landbo, A. K., Christensen, L. P., Hansen, A. and Meyer, A. S. 2001. Antioxidant Effects of Phenolic Rye (Secale cereale L.) Extracts, Monomeric Hydroxyl Cinnamates, and Ferulic acid Dehydrodimers on Human Low Density Lipoproteins. J. Agric. Food Chem., 49: 4090-4096.
3. Arabshahi, S. and Urooj, A. 2007. Antioxidant Properties of Various Solvent Extracts of Mulberry Morus indica L. Leaves. Food Chem., 102: 1233-1240.
4. Burt, S. 2004. Essential Oils: Their Antibacterial Properties and Potential Applications in Foods: A Review. Int. J. Food Microbiol., 94: 223-253.
5. Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Food Drug Anal., 10: 178-182.
6. Cowan, M. M. 1999. Plant Products as Antimicrobial Agents. Clinical Microbiology Review, 12: 564- 582.
7. Demirci, F., Guven, K., Demirci, B., Dadandi, M. Y. and Baser, K. H. C. 2008. Antibacterial Activity of Two Phlomis Essential Oils against Food Pathogens. Food Control, 19: 1159-1164.
8. Duffy, C. F. and Power, R. F. 2001. Antioxidant and Antimicrobial Properties of Some Chinese Plant Extracts. Int. J. Antimicrob. Ag., 17: 527-529.
9. Ebrahimabadi, A. H., Ebrahimabadi, E. H., Jafari-Bidgoli, Z., Jookar Kashi, F., Mazoochi, A. and Batooli, H. 2010. Composition and Antioxidant and Antimicrobial Activity of the Essential Oil and Extracts of Stachys inflata Benth from Iran. Food Chem., 119: 452-458.
10. Ghahreman, A. 1993. Iranian Chromophytes. First Edition, University Press, PP. 668-769.
11. Hsiao,G., Shen, M. Y. and Lin, K. H. 2003. Antioxdant and Hepatoproteceiv Effect of Antrodia camphorate Extract. J. Agric. Food Chem., 51: 3302-3308.
12. Khosravi, A. D. and Behzadi, A. 2006. Evaluation of the Antibacterial Activity of the Seed Hull of Quercus branti on Some Gram Negative Bacteria. Pak. J. Med. Sci., 22: 429-432.
13. Kilani, S., Abdelwahed, A., Ammar, R. B., Hayder, N., Ghedira, K., Chraief, I., Hammami, M. and Chekir-Ghedira, L. 2005 Chemical Composition, Antibacterial and Antimutagenic Activities of Essential Oil from (Tunisian) Cyperus rotundus. J. Essent. Oil Res., 17: 695-700.
14. Kochert, A. 1978. Carbohydrate Determination by Phenol-sulfuric Acid Method. In: "Handbook of Physiology and Biochemical Methods", (Eds.): Hellebust, J. A. and Craige, J. S.. Cambridge University Press, London, PP. 95-97.
15. Kotzekidou, P., Giannakidis, P. and Boulamatsis, A. 2008. Antimicrobial Activity of Some Plant Extracts and Essential Oils against Foodborne Pathogens In vitro and on the Fate of Inoculated Pathogens in Chocolate. LWT, 41: 119- 127.
16. Kumazawa, S., Taniguchi, M., Suzuki, Y., Shimura, M., Kwon, M. and Nakayama, T. 2002. Antioxidant Activity of Polyphenols in Carob Pods. Agric. Food Chem., 50: 373-377.
17. Lichtenthaler, H. K. 1987. Chlorophylls and Carotenoids; Pigments of Photosynthetic Membranes. Methods Enzaymol., 148: 350-382.
18. Majhenic, L., Skerget, M. and Knez, Z. 2007. Antioxidant and Antimicrobial Activity of Guarana Seed Extracts. Food Chem., 104: 1258–1268.
19. Meda, A., Lamien, C. E., Romito, M., Millogo, J. and Nacoulma, O. G. 2005. Determination of the Total Phenolic, Flavonoid and Pralin Contents in Burkina Fasan Honey, as well as Their Scavenging Activity. Food Chem., 91: 571-577.
20. Mita, S., Murano, N., Akaike, M. and Nakamura, K. 1997. Mutants of Arabidopsis thaliana with Pleiotropic Effects on the Expression of the Gene for Beta-amylase and on the Accumulation of Anthocyanin Those are Inducible by Sugars. Plant J., 11: 841-851.
21. Moure, A., Cruz, J. M., Franco, D., Dominguez, M., Sineiro, J. and Dominguez, H. 2001. Natural Antioxidants from Residual Sources. Food Chem., 72: 145-171.
22. NCCLS: National Committee for Clinical Laboratory Standards. 2001. Performance Standards for Anti-microbial Susceptibility Testing: Eleventh Informational Supplement. Document M 100-S11, National Committee for Clinical Laboratory Standard, Wayne, PA, USA.
23. Negi, P. S., Jayaprakasha, G. K. and Jena, B. S. 2003. Antioxidant and Antimutagenic Activities of Pomegranate Peel Extracts. Food Chem., 80: 393-397.
24. Oroojalian, F., Kasra-Kermanshahi, R. Azizi, M. and Bassami, M. R. 2010. Phytochemical Composition of the Essential Oils from Three Apiaceae Species and Their Antibacterial Effects on Food-borne Pathogens. Food Chem., 120: 765-770.
25. Pourmorad, F., Hosseinimehr, S. J. and Shahabimajd, N. 2006. Antioxidant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants. Afr. J. Biotechnol., 5: 1142-1145.
26. Sadeghi, H., Ghaitasi, I., Mazrooghi, N., and Sabzali, S. 2007. The Hepatoprotective Effects of Dorema auchri on Carbon Tetrachloride Induced Liver Damage in Rats. J. Shahrekord University. Med. Sci., 6(1): 38-43. (in Persian)
27. Salmanian, S., Sadeghi Mahoonak, A. R., Alami, M. and Ghorbani, M. 2014. Phenolic Content, Antiradical, Antioxidant, and Antibacterial Properties of Hawthorn (Crataegus elbursensis) Seed and Pulp Extract. J. Agr. Sci. Tech., 16: 343-354.
28. Shahidi, B. G., Aghighi, S. and Karimi Nik, A. 2004. Antibacterial and Antifungal Survey in Plants used in Indigenous Herbal-medicine of South East Regions of Iran. J. Biol. Sci., 4: 405 -412.
29. Sroka, Z. and Cisowski, W. 2003. Hydrogen Peroxide Scavenging, Antioxidant and Anti-radical Activity of Some Phenolic Acids. Food Chem. Toxicol., 41: 753-758.
30. Ten, M., Szentmihaly, K., Sarkozi, A. and Varga, I. S. 2003. Examination on Antioxidant Activity in Greater Celandine (Chelidonium majus L.) Extracts by FRAP Method. Acta Biol., 47: 115-117.
31. Wen, A., Delaquis, P., Stanich, K. and Toivonen, P. 2003. Antilisterial Activity of Selected Phenolic Acids. Food Microbiol., 56: 305-311.
32. Wollenweber, E., Dorr, M. and Rustaiyan, A. 1995. Dorema aucheri the First Umbelliferous Plant Found to Produce Exudates Flavonoids. Phytochem., 38(6): 1417-1427.
33. Zargari, A. 1997. Pharmaceutical Plants. Tehran University Press, PP. 212-221. (in Persian).
Journal of Agricultural Science and Technology
Volume 17, Issue 3
May and June 2015
Pages 623-634