Observations on the Cytology and Karyogram of an Onobrychis viciifolia Scop. New Variety in Callus, In vivo and In vitro Cultures

Authors
S. Mohajer
R. M. Taha
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Abstract
Genus Onobrychis is an important forage crop consisting of approximately 130 annual and perennial species. The present research focused on the cytological studies of Embryogenic (E) and Non-Embryogenic (NE) callus as well as root meristem cells of in vitro and in vivo grown plants. On the contrary to the previous studies, a diploid viciifolia variety with 2n= 2x= 16 chromosomes was observed for the first time in Onobrychis viciifolia ScopSyn. Onobrychis sativa L. Mitotic division was in general regular for all the growth conditions, but some such division irregularities as cytomixis, binucleate cells, micronucleus, asynchronous nucleus and chromosome bridge were also observed particularly in non-embryogenic callus samples. Ratio of nuclear to cell area of callus was slightly less than those in in vivo and in vitro values. Polyploidy level of DNA (C-value) was only indicated in callus. G1 had the highest percentage of nucleus among different stages of the interphase in in vitro and in vivo growth cultures. Karyotypic formula was 10 m+6 sm and a secondary constriction was distinguished in the short arm of the second pair chromosomes. Ultimately, scanning electron microscopy was employed to differentiate between root cells of the in vitro and in vivo growth systems.

Keywords

1. Abou-El-Enain, M. M. 2002. Chromosomal Criteria and Their Phylogenetic Implications in the Genus Onobrychis mill. sec. Lophobrychis (Leguminosae), with Special Reference to Egyptian Epecies. Bot. J. Linn. Soc., 139: 409-414.
2. Aksoy, O., Ekici, N. and Dane, F. 2008 Mitotic Changes in Root Meristems of Lens culinaris Treated with Fusillade (Fluazifop-p-Butyl), Asian J. Cell Biol., 3(1): 34-40.
3. Aktoklu, E. 2001. Two New Varieties and a New Record in Onobrychis from Turkey. Turk. J. Bot., 25: 359-363.
4. Arano, H. 1963. Cytological Studies in Subfamily Carduoideae (Compositae) of Japan. IX. The Karyotype Analysis and Phylogenetic Considerations on Pertya and Ainsliaea. The Botanical Magazine Tokyo, 76: 32–39.
5. Ashurmetov, O. A. and Normatov, B. A. 1998. Embryology of Annual Species of the Genus Onobrychis mill. Flora, 193: 259-267.
6. Baltisberger, M. 1991. IOPB Chromosome Data. 3. IOPB Newsletter, 17: 5-7.
7. De Wet, J. M. 1979. Origins of Polyploids. Basic Life Sci., 13: 3-15.
8. Diaz Lifante, Z., Luque, T. and Santa Barbara, C. 1992. Chromosome Numbers of Plants Collected during Iter Meditranium II in Israel. Bocconea, 3: 229–250.
9. Dolezel, J., Greilhuber, J. and Suda, J. 2007. Estimation of Nuclear DNA Content in Plants Using Flow Cytometry. Nature Protocols, 2: 2233-2244.
10. Duman, H. and Vural, M. 1990. New Taxa from South Anatolia 1. Turk. J. Bot., 14: 45-48.
11. Ekanem, A. M. and Osuji, J. O. 2006. Mitotic Index Studies on Edible Cocoyams (Xanthosoma and Colocasia spp.), African J. Biotech, 5(10): 846–849.
12. Evans, L. S. and Van't Hof, J. 1974. Is the Nnuclear DNA Content of Mature Root Cells Prescribed in the Root Meristem. Am. J. Bot., 61: 1104-1111.
13. Falistocco, E., Tosti, T. and Falcinelli, M. 1995. Cytomixis in Pollen Mother Cells of Diploid Dactylis, One of the Origins of 2n Gametes. Heredity, 86: 448-453.
14. Fukui, K. and Nakayama, S. 1996. Plant Chromosomes: Laboratory Methods. CRC Press. Boca Raton, pp, 241-255.
15. Gaspar, T., Kevers, C., Bisbis, B., Franck, T., Crevecoeur, M., Greppin, H. and Dommes, J. 2000. Loss of Plant Organ Ogenictotipotency in the Course of In vitro Neoplastic Progression. In Vitro Cell. Dev. Biol. Plant, 36: 171-18.
16. Ghanavatia, F. Nematpajoohb, N. Chahlic, K. and Safaeichaeikar, S. 2012. Cytological Evaluation of Annual Species of the Onobrychis genus in Iran, Crop Breed. J., 2(1): 17-24.
17. Goldblatt, P. 1981. Cytology and the phylogeny of Leguminosae. Part 2. In: "Advances in Legume Systematics", (Eds.): Polhill R. M. and Raven P. H.. Royal Botanic Gardens, Kew, PP. 427-463.
18. Grant, W. F. 1978. Chromosome Aberrations in Plants as Monitoring System. Environ. Health Perspect., 27: 37-43.
19. Greilhuber, J. and Speta, F. 1976. C-banded Karytypes in the Scilla hohenackeri Group, S. persic and Puschkinia (Liliaceae). Pl. Syst. Evol., 12: 149–188.
20. Gupta, P. K. and Durzan, D. J. 1987. Biotechnology of Somatic Poly Embryogenesis and Plantlet Regeneration in Loblolly Pine. Biotechnol., 5: 147 – 151.
21. Hayot-Carbonero, C., Carbonero, F., Smith, L. M. J. and Brown, T. A. 2013. Cytological Characterization of the Underutilized Forage Crop Onobrychis viciifolia scop. and Other Members of the Onobrychis genus. Genet. Resour. Crop Evol., 60: 1987–1996.
22. Hesamzadeh Hejazi, S. M. and Ziaei Nasab, M. 2009. Cytogenetic Study on Several Populations of Diploid Species of Onobrychis in Natural Gene Bank of Iran. Iranian J. Range. Forests Plant Breed. Genetic Res., 16(2): 158-171.
23. Hesam Zadeh Hejazi, S. M. and Ziaei Nasab, M. 2010. Cytotaxonomy of Some Onobrychis (Fabaceae) Species and Populations in Iran. Caryologia, 63 (1): 18- 31.
24. Hess, D. 1999. Herbicide Characteristics and Classification. Revised from Gerst, J. R., CWC Chemical, Inc., PP. 2-18.
25. Huziwara, Y. 1962. Karyotype Analysis in Some Genera of Compositeae. VIII. Further Studies on the Chromosome of Aster. Amer. J. Bot., 49: 116-119.
26. Karshibaev, H. K. 1992. Chromosome Numbers of Some Fabaceae in Uzbekistan. Tezisy, 3 Soveshchanie Po Kariologii Ratenii, 27: 1-2.
27. Khatoon, S. Ali, S. and Khatoon, S. 1991. Chromosome Numbers in Subfamily Papilionoideae (Leguminosae) from Pakistan. Willdenowia, 20: 159-165.
28. Kidambi, S. P., Mahan, J. R. and Matches, A. G. 1990. Purification and Thermal Dependence of Glutathione Reductase from Two Forage Legume Species. Plant Physiol., 92: 363-367.
29. Koduru, P. R. K. and Rao, M. K. 1981. Cytogenetics of Synaptic Mutants in Higher Plants. Theor. Appl. Genet., 59: 197-214.
30. Lavania, U. C. and Srivastava, S. 1992. A Simple Parameter of Dispersion Index that Serves as a Adjunct to Karyotype Asymmetry, J. Biosci., 17(2):179-182.
31. Levan, A. Fredga, K. and Sandberg, A. A. 1964. Nomenclature for Centromeric Position on Chromosomes. Hereditas, 52: 201-220.
32. Mansour, K. S. 1984. Cytological Effects of the Herbicide Tribunile on Victa faba. Egypt. J. Bot., 27: 191-198.
33. Marfil, C. F., Masuelli, R. W., Davison, J. and Comai, L. 2006. Genomic Instability in Solanum tuberosum×Solanum kurtzianum Interspecific Hybrids. Genome, 49: 104-113.
34. Mesicek, J. and Sojak, J. 1992. Chromosome Numbers of Mongolian Angiosperms. Preslia, 64: 193-206.
35. Miller, J. S. and Venable, D. L. 2000. Polyploidy and the Evolution of Gender Dimorphism in Plants. Sci., 289: 2335-2338.
36. Moghaddam, B. and Taha, R. M. 2008. Analysis of Cytological Activities in Sugar Beet Calluses, J. Biol. Sci., 8(5): 874-881
37. Mohajer, S., Taha, R. M., Khorasani, A and Syafawati Yaacob, J. 2012. Induction of Different Types of Callus and Somatic Embryogenesis in Various Explants of Sainfoin (Onobrychis viciifolia). Australian J. Crop Sci., 6(8):1305-1313.
38. Negri, V., Veronesi, F. and Falcinelli, M. 1987. Germplasm evaluation of Onobrychis viciifolia Scop. for agronomic traits. Genetica Agraria, 41: 25-40.
39. Nirmala, C. and Kaul, M. L. 1993. Male Sterility in Pea. IV. Meiotic Absence, Arrest, Breakdown and Continuation. Cytologia, 58: 247-255.
40. Otto, S. P. and Whitton, J. 2000. Polyploid Incidence and Evolution. Annu. Rev. Genetics, 34: 401-437.
41. Ranjbar, M., Karamian, R. and Hadadi, A. 2010. Cytosystematics of Three Onobrychis Species (Fabaceae) in Iran. Caryologia, 63(3): 237-249.
42. Ranjbar, M., Karamian, R. and Hajmoradi, F. 2009. Taxonomic Notes on Onobrychis sect. Hymenobrychis (Fabaceae, Hedysareae) in Iran. Novon, 19: 215-218.
43. Romero Zarco, C. 1986. A New Method for Estimating Karyotype Asymmetry. Taxon, 36: 526-530.
44. Seijo, G. and Fernadez, A. 2001. Cytogenetic Analysis of Lathyrus japonicus Willd. (Leguminosae). Caryologia, 54: 173-179.
45. Slavivk, B., Jarolivmovav, V. and Chrtek, J. 1993. Chromosome Counts of Some Plants from Cyprus. Candollea, 48: 221-230.
46. Stace, C. A. 2000. Cytology and Cytogenetics as a Fundamental Taxonomic Resource for the 20th and 21th Centuries. Taxon., 49: 451-477.
47. Stebbins, G. L. 1974. Flowering Plants: Evolution above the Species Level. Edward Arnold Publication Ltd., London, PP. 133-134.
48. Stevens, J. P. and Bougourd, S. M. 1991. The Frequency and Meiotic Behaviour of Structural Chromosome Variants in Natural Populations of Allium schoenoprasum L. (Wild Cchives) in Europe. Heredity, 66: 391-401.
49. Taha, R. M. and Wafa, S. N. 2012. Plant Regeneration and Cellular Behaviour Studies in Celosia cristata Grown In vivo and In vitro. The Scientific World Journal. Article ID 359413, 8 pages. doi: 10.1100/2012/359413.
50. Takhtajan, A. L. 1990. Numeri Chromosomatum Magnoliophytorum Florae. Acera-ceae-Menyanthaceae, URSS, 508 PP.
51. Tamas, E. 2006. Citological Aspects of the Onobrychis Genus. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Agriculture, 62.
52. Thomas, J. E. and Davidson, D. 1983. Cell and Nuclear Size in Viciafabaroots: Changes during Germination and in Response to Levels of Ambient Water. Annu. Bot., 51(3): 353-361.
53. Utsunomiya, K. S., Bione, N. C. and Pagliarini, M. S. 2002. How Many Different Kinds of Meiotic Abnormalities Could Be Found in a Unique Endogamous Maize Plant? Cytologia, 67: 169-176.
54. Verma, D. P. S. 2001. Cytokinesis and Building of the Cell Plate in Plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 52: 751-784.
55. Watanabe, K., Yahara, T., Denda, T. and Kosuge, K. 1999. Chromosomal Evolution in the Genus Brachyscome (Asteraceae, Astereae): Statistical Tests Regarding Correlation between Changes in Karyotype and Habit Using Phylogenetic Information. J. Plant Res., 112: 145–161.
56. Werner, A., Disanza, A., Reifenberger, N., Habeck, G., Becker, J., Calabrese, M., Urlaub, H., Lorenz, H., Schulman, B., Scita, G. and Melchior, F. 2013. SCF-Fbxw5 Mediates Transient Degradation of Act in Remodeller Eps8 to Allow Proper Mitotic Progression. Nature Cell Biol., doi:10.1038/ncb266
57. Yan-Jun, Z., Meng, A., Li, J., Dang, H. and Li, X. 2006. Observation on Meiotic Behavior in three Mahonia Species, with Special Reference to the Intergeneric Relationship of Mahonia and Berberis. Caryologia, 59: 305–311.
58. Yildiz, B., Ciplak, B. and Aktoklu, E. 1999. Fruit Morphology of Sections of the Genus Onobrychis Miller (Fabaceae) and Its Phylogenetic Implications. J. Plant Sci., 47: 269-282.
Journal of Agricultural Science and Technology
Volume 16, Issue 7
November and December 2014
Pages 1683-1698