Inhibitory Activity of Plant Extracts on Aflatoxin B1 Biosynthesis by Aspergillus flavus

Authors
Department of Microbiology and Biotechnology, Bangalore University, Bangalore–560056, India.
Abstract
The inhibitory activities of aqueous and solvent extracts of twelve selected medicinal plants were evaluated against biosynthesis of aflatoxin B1 (AFB1) by Aspergillus flavus. The A. flavus was isolated from maize, and aflatoxin B1 biosynthesis was confirmed by comparison with standard AFB1 using TLC method. In vivo antiaflatoxigenic efficacies of activity guided solvent extracts were determined in maize model system. All the extracts showed varying degree of antifungal and AFB1 inhibitory activities, but chloroformic extract of Albizia amara, Cassia spectabilis and Solanum indicum, and methanolic extract of Acacia catechu, Albizia saman andAnogeissus latifolia showed the highest activity. Further investigations on identification of active principles from these plants are needed to develop plant based formulations for management of A. flavus growth and AFB1 contamination in food grains.

Keywords


1. Ahmed, S. M., Ahmed, S., Tasleem, F., Hasan, M. M. and Azhar, I. 2012. Acute Systemic Toxicity of Four Mimosaceous Plants Leaves in Mice. IOSR J. Pharm., 2(2): 291-295.
2. Ajam, S. M. S., Salleh, B., Al-khalil, S. and Sulaiman, S.F. 2012. Antimicrobial Activity of Spermine Alkaloids from Samanea saman against Microbes Associated with Sick Buildings. Int. Conf. Environ. Chem. Biol., 49: 150-155.
3. Ara, A., Arifuzzaman, M., Ghosh, C.K., Hashem, M. A., Ahmad, M. U., Bachar, S. C., Nahar, L. and Sarker, S. D. 2010. Anti-inflammatory Activity of Adenanthera pavonina L., Fabaceae, in Experimental Animals. Braz., J. Pharmacog., 20(6): 929-932.
4. Arulpriya, P., Lalitha, P. and Hemalatha, S. 2010. In vitro Antioxidant Testing of the Extracts of Samanea saman (Jacq.) Merr. Der. Chemica. Sinica. 1: 73-79.
5. Ashokkumar, R., Perumal, G. and Ramaswamy, M. A. 2012. Comparative Study on the Antimicrobial Activity of Normal and Galled Leaves of Five Medicinal Plants. Int. J. Biol. Technol., 3(2): 33-36.
6. Azhar, I., Hasan, M. M., Mazhar, F. and Ali, M. S. 2009. Some Biological Evaluations on Samanea saman. Pak. J. Pharmacol., 26: 47-53.
7. Bhardwaj, S. K. and Laura, J. S. 2009. Antibacterial Activity of Some Plant-Extracts against Plant Pathogenic Bacteria Xanthomonas campestris pv. Campestris. Indian J. Agric. Res., 43(1): 26-31.
8. Das, P. K., Mondal, A. K. and Parui, S. M. 2011. Antibacterial Activity of Some Selected Dye Yielding Plants in Eastern India. African J. Plant Sci., 5(9): 510-520.
9. Deng, Y., Yu, Y., Luo, H., Zhang, M., Qin, X. and Li, L. 2011. Antimicrobial Activity of Extract and Two Alkaloids from Traditional Chinese Medicinal Plant Stephania dielsiana. Food Chem., 124: 1556-1560.
10. Dowling, F. S. 1997. Fumonisin and Its Toxic Effects. Cereal Foods World, 42: 13-15.
11. Ferdous, A., Imam, M. Z. and Ahmed, T. 2010. Antioxidant, Antimicrobial and Cytotoxic Activities of Samanea saman (Jacq.) Merr. S. J. Pharma. Sci., 3: 11-17
12. Garcia, D., Ramos, A.J., Sanchis, V. and Marin, S. 2012. Effect of Equisetum arvense and Stevia rebaudiana Extracts on Growth and Mycotoxin Production by Aspergillus flavus and Fusarium verticillioides in Maize Seeds as Affected by Water Activity. Int. J. Food Microbiol., 153: 21-27.
13. Hajji, M., Masmoudi, O., Souissi, N., Triki, Y., Kammoun, S. and Nasri, M. 2010. Chemical Composition, Angiotensin I-converting Enzyme (ACE) Inhibitory, Antioxidant and Antimicrobial Activities of the Essential Oil from Periploca laevigata Root Barks. Food Chem., 121: 724-731.
14. Hishobkar, S. M., Urolagin, D. K., Ashish C. and Maski, S. 2010. Evaluation of Synergestic Antimicrobial Effect of Anogeissus latifolia and Glycerrhiza glabra Extract. Int. J. Pharm. Pharma. Sci., 2(4): 158-159.
15. Hussain, A., Rizvi, A., Wahab, S., Zareen, I., Ansari, S. and Hussain, M. S. 2011. Antibacterial Screening of the Bark of Adenanthera pavonina (L.). Int. J. Biomed. Res., 2(2): 110-122.
16. Ikram, N. and Dawar, S. 2013. Effect of Prosopis juliflora (SW.) DC. in the Control of Root Rot Fungi of Cowpea (Vigna unguiculata L.) and Mung Bean [Vigna radiata (L.) Wilczek]. Pak. J. Bot., 45(2): 649-654.
17. ISTA. 1996. International Rules for Seed Testing. Seed Sci. Techechnol., 21(1): 25-30.
18. Joshi, S., Subedi, Y. P. and Paudel, S. K. 2011. Antibacterial and Antifungal Activity of Heartwood of Acacia catechu of Nepal. J. Nepal. Chem. Soc., 27: 94-99.
19. Karmegam, N., Jayakumar, M. and Karuppuswamy, S. 2012. Synergistic Antibacterial Activity of Four Medicinal Plants Collected from Dharapuram Taluk of Tiruppur District, South India. J. Plant Sci., 7: 32-38.
20. Kumar, R., Mishra, A. K., Dubey, N. K. and Tripathi, Y. B. 2007. Evaluation of Chenopodium ambrosioides Oil as a Potential Source of Antifungal, Antiaflatoxigenic and Antioxidant Activity. Int. J. Food Microbiol., 115: 159-164.
21. Mahida, Y. and Mohan, J. S. S. 2007. Screening of Plants for Their Potential Antimicrobial Activity against Staphylococcus and Salmonella spp. Nat. Prod. Rad., 6(4): 301-305.
22. Manafi, M. and Khosravinia, H. 2013. Effects of Aflatoxin on the Performance of Broiler Breeders and Its Alleviation through Herbal Mycotoxin Binder. J. Agr. Sci. Tech. 15: 55-63.
23. Mohana, D. C. and Raveesha, K. A. 2007. Anti-fungal Evaluation of Some Plant Extracts against Some Plant Pathogenic Field and Storage Fungi. J. Agri. Technol., 4(1): 119-137.
24. Mohana, D. C. and Raveesha, K. A. 2010. Antimycotic, Anti-biodeteriorative and Antiaflatoxigenic Potency of 2-hydroxy-4-methoxybenzaldehyde Isolated from Decalepis hamiltonii on Fungi Causing Biodeterioration of Maize and Sorghum Grains. J. Mycol. Plant Pathol., 40(2): 197-206.
25. Nnamdi, L. O., Anthony, C. C. E., Pius, O. U. and Paul, M. E. 2010. Comparative Phytochemical and Antimicrobial Screening of Some Solvent Extracts of Samanea saman (Fabaceae or Mimosaceae) Pods. Afr. J. Pure Appl. Chem., 4: 206-212
26. Negi, B. S. and Dave, B. P. 2010. In vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis. Indian J. Microbiol., 50(4): 369–374.
27. Passone, M. A., Resnik, S. and Etcheverry, M. G. 2008. The Potential of Food Grade Antioxidants in the Control of Aspergillus Section Flavi, Interrelated Mycoflora and Aflatoxin B1 Accumulation on Peanut Grains. Food Control, 19: 364-371.
28. Pirzada, A., Shaikh, W. Usmanghani, K. and Mohiuddin, E. 2010. Antifungal Activity of Dodonaea viscosa Jacq. Extract on Pathogenic Fungi Isolated from Super Ficial Skin Infection. Pak. J. Pharm. Sci., 23(3): 337-340.
29. Prasad, R. N., Viswanathan, S., Devi, J. R., Nayak, V., Swetha, V. C., Archana, B. R., Parathasarathy, N. and Rajkumar, J. 2008. Preliminary Phytochemical Screening and Antimicrobial Activity of Samanea saman. J. Med. Plants Res., 2: 268-270.
30. Praveen, P., Thippeswamy, S., Mohana, D. C. and Manjunath, K. 2011. Antimicrobial Efficacy and Phytochemical Analysis of Albizia amara (Roxb.) Boiv. an Indigenous Medicinal Plant against Some Human and Plant Pathogenic Bacteria and Fungi. J. Pharm. Res. 4(3): 832-835.
31. Quiroga, E. N., Sampietro, D. A., Sgariglia, M. A., Soberon, J. R. and Vattuone, M. A. 2009. Antimycotic Activity of 5′-prenylisoflavanones of the Plant Geoffroea decorticans, against Aspergillus Species. Int. J. Food Microbiol., 132: 42-46.
32. Raghavendra, M. P., Satish, S. and Raveesha, K. A. 2008. In vitro Antibacterial Potential of Alkaloids of Samanea saman (Jacq.) Merr. against Xanthomonas and Human Pathogenic Bacteria. World J. Agri. Sci., 4: 100-105
33. Rahimi, P., Sharifnabi, B. and Bahar, M. 2008. Detection of Aflatoxin in Aspergillus Species Isolated from Pistachio in Iran. J. Phytopathol., 156: 15-20.
34. Reddy, K. R. N., Reddy, C. S. and Muralidharan, K. 2009. Detection of Aspergillus spp. and Aflatoxin B1 in Rice in India. Food Microbiol., 26: 27-31.
35. Salari, R., Najafi, M. B. H., Boroushaki, M. T., Mortazavi, S. A. and Najafi, M. F. 2012. Assessment of the Microbiological Quality and Mycotoxin Contamination of Iranian Red Pepper Spice. J. Agr. Sci. Tech., 14: 1511-1521.
36. Sangetha, S., Zuraini, Z., Sasidharan, S. and Suryani, S. 2008. Fungicidal Effect and Oral Toxicity of Cassia spectabilis Leaf Extract. Jpn. J. Med. Mycol., 49: 299-304.
37. Satish, S., Mohana, D. C., Raghavendra, M. P. and Raveesha, K. A. 2007. Antifungal Activity of Some Plant Extracts against Important Seed Borne Pathogens of Aspergillus sp. J. Agri. Technol. 3(1): 109-119.
38. Shukla, R., Kumar, A., Prasad, C. S., Srivastava, B. and Dubey, N. K. 2008. Antimycotic and Antiaflatoxigenic Potency of Adenocalymma alliaceum Miers. on Fungi Causing Biodeterioration of Food Commodities and Raw Herbal Drugs. Int. Biodeter. Biodegr., 62: 348-351.
39. Shukla, R., Kumar, A., Singh. P. and Dubey, N. S. 2009. Efficacy of Lippa alba (Mill.) N. E. Brown Essential Oil and Its Monoterpene Aldehyde Constituents against Fungi Isolated from Some Edible Legume Seeds and Aflatoxin B1 Production. Int. J. Food Microbiol., 135: 165-170.
40. Siva, R., Palackan, M.G., Maimoon, L., Geetha, T., Bhakta, D., Balamurugan, P. and Rajanarayanan S. 2011. Evaluation of Antibacterial, Antifungal, and Antioxidant Properties of Some Food Dyes. Food Sci. Biotechnol., 20(1): 7-13.
41. Singh, K., Frisvad, J. C., Thrane, U. and Mathur, S. B. 1991. An Illustrated Manual on Identification of Some Seed-borne Aspergilli, Fusaria, Penicillia and Their Mycotoxins. 1st Edition, Danish Government Institute of Seed Pathology for Developing Countries, Hellerup, Denmark, PP.124-127.
42. Sparg, S.G., Kulkarni, M.G., Light, M.E. and Staden J.V. 2005. Improving Seedling Vigour of Indigenous Medicinal Plants with Smoke. Bioresource Technol., 96: 1323-1330.
43. Thippeswamy, S., Praveen, P., Mohana, D. C. and Manjunath, K. 2011, Antimicrobial Evaluation and Phytochemical Analysis of Known Medicinal Plant Samanea saman (Jacq.) Merr. against Some Human and Plant Pathogenic Bacteria and Fungi. Int. J. Pharma Bio Sci., 2(2): 443-452.
44. Verma, J. and Dubey, N. K. 1999. Prospectives of Botanical and Microbial Products as Pesticides of Tomorrow. Current Sci., 76: 172-179.