Chitinolytic Activity of Native Pseudomonas fluorescens Strains

Authors
R. Nandakumar 1
S. Babu 1
T. Raguchander 2
R. Samiyappan 1
1 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 640 003, India.
2 Sugarcane Research Station, Semmandalam, Cuddalore 607 001, Tamil Nadu, India.
Abstract
The chitinase producing ability of Pseudomonas fluorescens strains viz., PF1, PB2 and FP7 was evaluated in a culture medium with and without a chitin source. The addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP7 responded well to the addition of chitin by producing 31.2% increased chitinase in culture. Western blot analysis with chitinase antibody detected six and five chitinase isoforms in culture inoculated with FP7 and PF1, respectively.

Keywords

Journal of Agricultural Science and Technology
Volume 9, Issue 1
January and February 2007
Pages 61-68