1
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 640 003, India.
2
Sugarcane Research Station, Semmandalam, Cuddalore 607 001, Tamil Nadu, India.
Abstract
The chitinase producing ability of Pseudomonas fluorescens strains viz., PF1, PB2 and FP7 was evaluated in a culture medium with and without a chitin source. The addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP7 responded well to the addition of chitin by producing 31.2% increased chitinase in culture. Western blot analysis with chitinase antibody detected six and five chitinase isoforms in culture inoculated with FP7 and PF1, respectively.
Nandakumar, R., Babu, S., Raguchander, T. & Samiyappan, R. (2007). Chitinolytic Activity of Native Pseudomonas fluorescens Strains. Journal of Agricultural Science and Technology, 9(1), 61–68.
MLA
Nandakumar, R., Babu, S., Raguchander, T., & Samiyappan, R. "Chitinolytic Activity of Native Pseudomonas fluorescens Strains", Journal of Agricultural Science and Technology, 9, 1, 2007, 61–68.
HARVARD
Nandakumar, R., Babu, S., Raguchander, T., & Samiyappan, R. (2007). 'Chitinolytic Activity of Native Pseudomonas fluorescens Strains', Journal of Agricultural Science and Technology, 9(1), pp. 61–68.
CHICAGO
Nandakumar, R., Babu, S., Raguchander, T. & Samiyappan, R., "Chitinolytic Activity of Native Pseudomonas fluorescens Strains." Journal of Agricultural Science and Technology, 9 1 (2007): 61–68,
VANCOUVER
Nandakumar R., Babu S., Raguchander T., Samiyappan R. Chitinolytic Activity of Native Pseudomonas fluorescens Strains. J. Agric. Sci. Technol. 2007;9(1):61–68.